Depressed immunity to a Salmonella typhimurium vaccine in mice experimentally parasitized by Taenia crassiceps

Rubio, Miguel; Tato, Patricia; Govezensky, Tzipe; Molinari, José Luis

doi:10.1016/s0304-4017(97)00173-8

Cited by 10 publications

(10 citation statements)

References 38 publications

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“…However, until the last few years was that several researchers demonstrated the direct effect of the presence of this cestode in modulating the immune response and susceptibility to other infections. Thus, previously T. crassicepsinfected mice are refractory to successful vaccination against Salmonella [65]. Likewise, mice carrying T. crassiceps had a differential response to a co-infection with Trypanosoma cruzi, if early co-infection was carried out, a minor susceptibility to the co-infection was observed [66], conversely, when co-infection was performed after 4 weeks of T. crassiceps infection higher T. cruzi parasitemia was detected in co-infected mice.…”

Section: Immunomodulation In Cysticercosismentioning

confidence: 58%

The Complex Role of Pro- and Anti-Inflammatory Cytokines in Cysticercosis:Immunological Lessons from Experimental and Natural Hosts

Terrazas¹

2008

CTMC

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Parasitic helminthes have developed complex mechanisms to evade or modulate hosts responses. Studies on cysticercosis are solid, but scarce. The most studied immunological models of cysticercosis are Taenia crassiceps infecting mice and T. solium infecting pigs. These parasites, despite being widely exposed to the host, are able to modulate the host immune system. Taenia metacestodes, much like other helminthes parasites, have developed complicated strategies in order to infect and successfully colonize their hosts. We focus here on the accumulated evidence from experimental models that have been helpful in analyzing and characterizing the host immune response to cysticercosis. Moreover, the mouse model has been used to design rationale vaccine strategies, some of them with promising results. We also discuss recent advances in understanding immune-regulation of cysticercosis. The parasite is able to manipulate the host immune system into supporting its survival by keeping a low inflammatory profile by causing the production of some cysticerci-released products that have immunomodulatory activities, as well as promoting the raise of alternatively activated macrophages. Finally, we delineate, according to recent literature, the likely pathway involved in protection and susceptibility against cysticercosis. As more aspects of the role of different immune and parasite-derived molecules are elucidated, better therapeutic targets may be identified to help treat cysticercosis.

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Section: Immunomodulation In Cysticercosismentioning

confidence: 58%

The Complex Role of Pro- and Anti-Inflammatory Cytokines in Cysticercosis:Immunological Lessons from Experimental and Natural Hosts

Terrazas¹

2008

CTMC

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“…The high level of depletion of effector cells such as eosinophils by apoptosis and T and B cells by unknown causes induced by the parasite or their secreted products may be the cause of immunosuppression in mice infected with T. crassiceps cysticerci, as described by others (Good and Miller 1976;Sciutto et al 1995;Villa and Kuhn 1996;Rubio et al 1998). Apoptosis of CD4+ cells induced in vitro by a T. solium cysteine protease has been reported by Tato et al (2004) and apoptosis of lymphocytes in the inflammatory reaction around cysticerci of T. solium in porcine cysticercosis (Solano et al 2006;Sikasunge et al 2008).…”

Section: Discussionmentioning

confidence: 87%

“…Moreover, in a field trial performed to evaluate a vaccine against porcine cysticercosis, we found that peripheral blood lymphocytes from cysticercotic pigs exhibited a depressed proliferative response to concanavalin-A (Molinari et al 1993). It has also been reported that infections of Balb/c mice with T. crassiceps result in an immunosuppressed stage (Sciutto et al 1995;Villa and Kuhn 1996;Rubio et al 1998).…”

Section: Introductionmentioning

confidence: 77%

Decrease of peritoneal inflammatory CD4+, CD8+, CD19+ lymphocytes and apoptosis of eosinophils in a murine Taenia crassiceps infection

et al. 2010

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After an intraperitoneal infection of mice with Taenia crassiceps metacestodes, peritoneal inflammatory cells labeled with fluoresceinated MoAb anti-mouse were analyzed by flow cytometry. Apoptosis was studied by annexin A/PI, TUNEL assays, DNA laddering, caspase-3 activity, and electron microscopy. An important continuous decrease of CD4+, CD8+ and CD19+ lymphocytes, and an increase of eosinophils and macrophages throughout the observation time were found. Apoptosis of eosinophils was quantified during the observation period with a peak at 6 days post-infection (67.27%). In an additional experiment at 12 days post-infection using TUNEL staining, a high level of apoptosis of eosinophil (92.3%) and a significant decrease of CD4+, CD8+, and CD19+ lymphocytes were confirmed. Caspase-3 activity in peritoneal fluid, peritoneal cells' DNA fragmentation, and apoptosis of eosinophils and monocytes were found. The dramatic decrease of peritoneal inflammatory T and B cells and the high level of apoptosis of inflammatory eosinophils induced in mice by infection with T. crassiceps cysticerci may be important factors of the immunosuppression observed in cysticercosis.

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“…On the other hand, Rubio et al (1998) found that IgG antibodies to Salmonella typhimurium were significantly diminished in mice parasitized by T. crassiceps and immunized with S. typhimurium antigens, suggesting that T. crassiceps down-regulates antibody production. Other data have shown that T. crassiceps larvae release factors in early infection that are capable of down-regulating proliferative responses and cytokine production by CD4 + cells (Spolski et al 2000).…”

Section: Introductionmentioning

confidence: 91%

The implantation of Taenia solium metacestodes in mice induces down-modulation of T-cell proliferation and cytokine production

et al. 2005

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The purpose of this study was to determine the effect of the implantation of Taenia solium metacestodes and the treatment with suppressive metacestode factor (F1) on the ability of spleen cells from Balb/c mice to produce cytokines. Cytokine production was estimated 12 days following the implantation or 4 days after the last dose of F1 (five doses) by RT-PCR and flow cytometry analyses. Spleen cells were obtained from metacestode-implanted, F1-treated and control mice. They were stimulated with concanavalin A (ConA) ex vivo and used for RT-PCR studies and for CD25 expression and intracellular cytokine production estimations using specific monoclonal antibodies labeled with phycoerithrin or fluorescein. Results of the RT-PCR showed that all cells expressed IFN-gamma, IL-2 and IL-4 mRNAs. IL-10 mRNA was not expressed in any case. Flow cytometry analyses showed that both spleen CD4+ and CD8+ cells from metacestode-implanted or treated-F1 mice expressed significantly diminished percentages of CD25 when compared with control cells (P<0.05). The estimation of intracellular cytokines showed that the production of IL-2 and IL-4 in CD8+ cells, and of IFN-gamma in CD4+ cells from mice implanted with metacestodes was significantly impaired when compared with the values from control cells (P<0.05).

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Depressed immunity to a Salmonella typhimurium vaccine in mice experimentally parasitized by Taenia crassiceps

Cited by 10 publications

References 38 publications

The Complex Role of Pro- and Anti-Inflammatory Cytokines in Cysticercosis:Immunological Lessons from Experimental and Natural Hosts

The Complex Role of Pro- and Anti-Inflammatory Cytokines in Cysticercosis:Immunological Lessons from Experimental and Natural Hosts

Decrease of peritoneal inflammatory CD4+, CD8+, CD19+ lymphocytes and apoptosis of eosinophils in a murine Taenia crassiceps infection

The implantation of Taenia solium metacestodes in mice induces down-modulation of T-cell proliferation and cytokine production

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