Depot‐Specific Expression of Fibroblast Growth Factors in Human Adipose Tissue

Abstract

Research Methods and Procedures:Adipose tissue (sc and om) was obtained from obese men. Gene expression was analyzed by DNA microarrays in triplicate (n ϭ 6) or by real-time polymerase chain reaction (n ϭ 9). Results: FGF-1, FGF-2, FGF-7, FGF-9, FGF-10, and FGF-18 transcripts were detected in human adipose tissue. The expression of FGF-2, FGF-7, and FGF-10 was similar in sc and om adipose tissue, whereas FGF-1 and FGF-9 were expressed at higher levels in the om adipose tissue. Expression of FGF-18 was only detected in om adipose tissue in two of the subjects. Analysis of cell fractions revealed that FGF-2 was only expressed in adipocytes; FGF-7, FGF-9, and FGF-18 were expressed in the stromavascular fraction; and FGF-1 and FGF-10 were expressed in both adipocytes and in the stroma-vascular fraction.FGFR-1 was expressed in both depots in all subjects and in both cell fractions, whereas FGFR-2 expression was undetectable in whole adipose tissue but detectable in the adipocyte fractions from both sc and om depots. Discussion: We show that several members of the FGF family are expressed in human adipose tissue, and that the expression for some of the FGFs differs between sc and om adipose tissue. Taken together with previously published reports on the biological effects of FGFs on adipose cells, our results suggest that locally expressed FGFs could play role in the regulation of regional adipose tissue mass.

Fat Depot-Specific Differences in Angiogenic Growth Factor Gene Expression and Its Relation to Adipocyte Size in Cattle

“…In contrast, Gabrielsson et al [13] showed that the expression levels of FGF-2 and FGF-10 mRNA were similar between subcutaneous and omental adipose tissues in humans. Therefore, the possibility cannot be ruled out that the difference between the results of Gabrielsson et al [13] and those of the current study could be, at least in part, due to the differences in mesenteric and omental anatomical sites. We also showed for the first time that the expression of FGFs mRNA in intramuscular, renal and intermuscular adipose tissue.…”

“…Fibroblast growth factor-2 (FGF-2) and fibroblast growth factor-10 (FGF-10) are also potent inducers of angiogenesis secreted by adipocytes [13,14,26]. Hepatocyte growth factor (HGF) is also secreted by adipocytes and stimulates angiogenesis [3,39].…”

Association of anti-obesity activity of N-acetylcysteine with metallothionein-II down-regulation

“…A variety of metabolic differences such as fatty acid turnover, lipolysis (van Harmelen et al, 2002), and the effectiveness of insulin action, in omental and subcutaneous adipose tissues have been reported (Wajchenberg et al, 2002). In addition, many depotrelated genes have been characterized by DNA array technology (Gabrielsson et al, 2002;Gabrielsson et al, 2003) and by differential display PCR (Montague et al, 1998). Inhibitor of apoptosis (Montague et al, 1998), lipoprotein lipase (Fried et al, 1993), leptin (Montague et al, 1997), glucose transporter 4, glycogen synthase, peroxisome proliferatoractivated receptor (PPAR)-γ (Lefebvre et al, 1998), angiotensin (Rahmouni et al, 2004), acylation stimulating protein, cholesterol ester transfer protein (Dusserre et al, 2000), and perillipin (Arvidsson et al, 2004) have all been reported to be differentially expressed in omental and subcutaneous adipose tissues.…”

Effects of Whole Crop Rice Silage on Meat Quality and Adipokine Gene Expression in Fattening <i>Wagyu</i> Cattle

“…Adipocyte secretes many adipokines, such as vascular endothelial growth factor (VEGF) and fibroblast growth factor-2 (FGF-2) (Gabrielsson et al 2002, MiyazawaHoshimoto et al 2005. Oxidative stress stimulates the expression of VEGF and FGF-2 genes in adipocytes (Lolmede et al 2003).…”