Deposition patterns of feruloylarabinoxylan during cell wall formation in moso bamboo

Munekata, Noriaki; Tsuyama, Taku; Kamei, Ichiro; Kijidani, Yoshio; Takabe, Keiji

doi:10.1007/s00425-022-03970-8

Cited by 8 publications

(9 citation statements)

References 59 publications

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“…Combining the results of the LCC-0 fractions with the NMR analysis, a high content of arabinose, H-type lignin structural units, and FA linkage structures in the LCC was observed, indicating their dissolution from the S-layer of the fibrous cell wall. Similar observations were reported by Munekata et al, who studied the formation patterns of the FA–arabinoxylan structure . Consequently, H-type lignin–FA–arabinoxylan recalcitrant structures can be concluded to predominantly exist in the S-layer of the bamboo fiber cell wall.…”

Section: Resultssupporting

confidence: 87%

“…Similar observations were reported by Munekata et al, who studied the formation patterns of the FA−arabinoxylan structure. 25 Consequently, H-type lignin−FA−arabinoxylan recalcitrant structures can be concluded to predominantly exist in the S-layer of the bamboo fiber cell wall.…”

Section: D Hsqc Nmr Spectrum Analysis Of Lc Bondsmentioning

confidence: 99%

“…However, whether the distribution of different chemical components within the cell wall influences the distribution of LCC remains unclear. Munekata et al demonstrated that the deposition of the FA–arabinoxylan recalcitrant structures is initiated during the formation of the S 2 -layer of the bamboo cell walls and reaches its highest concentration in the S-layer of mature bamboo . Although the deposition patterns of LCC structures within bamboo cell walls have been highlighted in previous studies, changes in the LCC structure during the green disassembly process of bamboo cell walls remain unclear.…”

Section: Introductionmentioning

confidence: 99%

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Unveiling the Dissolution Regularities of the Lignin–Carbohydrate Complex in Bamboo Cell Walls during Alkali Pretreatment

Wang,

Pu,

Liu

et al. 2024

J. Agric. Food Chem.

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Bamboo is a promising biomass resource. However, the complex multilayered structure and chemical composition of bamboo cell walls create a unique anti-depolymerization barrier, which increases the difficulty of separation and utilization of bamboo. In this study, the relationship between the connections of lignin−carbohydrate complexes (LCCs) within bamboo cell walls and their multilayered structural compositions was investigated. The chemical composition, structural properties, dissolution processes, and migration mechanisms of LCCs were analyzed. Alkali-stabilized LCC bonds were found to be predominantly characterized by phenyl glycoside (PhGlc) bonds along with numerous p-coumaric acid (PCA) linkage structures. As demonstrated by the NMR and CLSM results, the dissolution of the LCC during the alkaline pretreatment process was observed to migrate from the inner secondary wall (S-layer) of the bamboo fiber cell walls to the cell corner middle lamella (CCML) and compound middle lamella (CML), ultimately leading to its release from the bamboo. Furthermore, the presence of H-type lignin−FA−arabinoxylan linkage structures within the bamboo LCC was identified with their primary dissolution observed in the S-layer of the bamboo fiber cell walls. The study results provided a clear target for breaking down the anti-depolymerization barrier in bamboo, signifying a major advancement in achieving the comprehensive separation of bamboo components.

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Section: Resultssupporting

confidence: 87%

Section: D Hsqc Nmr Spectrum Analysis Of Lc Bondsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Unveiling the Dissolution Regularities of the Lignin–Carbohydrate Complex in Bamboo Cell Walls during Alkali Pretreatment

Wang,

Pu,

Liu

et al. 2024

J. Agric. Food Chem.

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“…Such compensatory response has been suggested to occur in wheat endosperm walls as a response to low arabinoxylan content (Freeman et al, 2017). Furthermore, xylan cross-linking has been reported to occur in the S2 layer of (Munekata et al, 2022), which would be consistent with SCW AXe also being feruloylated.…”

Section: Discussionmentioning

confidence: 95%

Altering the substitution and cross‐linking of glucuronoarabinoxylans affects cell wall architecture in Brachypodium distachyon

Tryfona,

Pankratova,

Petrik

et al. 2024

New Phytologist

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Summary The Poaceae family of plants provides cereal crops that are critical for human and animal nutrition, and also, they are an important source of biomass. Interacting plant cell wall components give rise to recalcitrance to digestion; thus, understanding the wall molecular architecture is important to improve biomass properties. Xylan is the main hemicellulose in grass cell walls. Recently, we reported structural variation in grass xylans, suggesting functional specialisation and distinct interactions with cellulose and lignin. Here, we investigated the functions of these xylans by perturbing the biosynthesis of specific xylan types. We generated CRISPR/Cas9 knockout mutants in Brachypodium distachyon XAX1 and GUX2 genes involved in xylan substitution. Using carbohydrate gel electrophoresis, we identified biochemical changes in different xylan types. Saccharification, cryo‐SEM, subcritical water extraction and ssNMR were used to study wall architecture. BdXAX1A and BdGUX2 enzymes modify different types of grass xylan. Brachypodium mutant walls are likely more porous, suggesting the xylan substitutions directed by both BdXAX1A and GUX2 enzymes influence xylan‐xylan and/or xylan–lignin interactions. Since xylan substitutions influence wall architecture and digestibility, our findings open new avenues to improve cereals for food and to use grass biomass for feed and the production of bioenergy and biomaterials.

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“…Small blocks (ca. 7 × 3 × 7 mm, vertical × tangential × radial) from the trunk disk of a Furano tree were prepared for immunolabeling of xylan and fluorescence microscopy as described previously (Munekata et al, 2022). The transverse sections from blocks embedded in LR white resin (LR White Resin Hard Grade Acryl Resin; London Resin Company, London, UK) were incubated with anti‐xylan monoclonal antibody (LM10, specific for xylan with unsubstituted or low substituted backbone; PlantProbes, Leeds, UK) and with secondary antibody (anti‐rat IgG Alexa Fluor 488; Invitrogen, Waltham, MA, USA).…”

Section: Methodsmentioning

confidence: 99%

Formation of xylem tissues and secondary cell walls is diminished by severe and consecutive insect defoliation

Watanabe,

Yamagishi,

Takata

et al. 2023

American J of Botany

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PremiseInsect defoliation of trees causes unusual changes to wood anatomy and slows radial growth that decreases tree value; however, the characteristics of these anatomical changes in hardwoods remain unclear. The aim of this study was to characterize the anatomy and histochemistry of the wood in trunks of Betula maximowicziana trees after severe insect defoliation.MethodsSecondary xylem tissues were sampled from trunks that had been defoliated by Caligula japonica at Naie and Furano in central Hokkaido during 2006‐2012, then cross‐dated and examined microscopically and stained histochemically to characterize anatomical and chemical changes in the cells.ResultsWhite rings with thin‐walled wood fibers and greatly reduced annual ring width in the subsequent year were observed in samples from both sites. From these results, the year that the white rings formed was determined, and severe defoliation was confirmed to trigger white ring formation. The characteristics may prove useful to detect the formation year of white rings. Scanning electron microscopy and histochemical analyses of the white rings indicated that the thickness of the S2 layer in the wall of wood fiber cells decreased, but xylan and lignin were still deposited in the cell walls of wood fibers. However, the walls of the fibers rethickened after the defoliation.ConclusionsOur results suggest that B. maximowicziana responds to a temporary lack of carbon inputs due to insect defoliation by regulating the thickness of the S2 layer of the cell wall of wood fibers. For B. maximowicziana, insect defoliation late in the growing season has serious deleterious effects on wood formation and radial growth.

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Deposition patterns of feruloylarabinoxylan during cell wall formation in moso bamboo

Cited by 8 publications

References 59 publications

Unveiling the Dissolution Regularities of the Lignin–Carbohydrate Complex in Bamboo Cell Walls during Alkali Pretreatment

Unveiling the Dissolution Regularities of the Lignin–Carbohydrate Complex in Bamboo Cell Walls during Alkali Pretreatment

Altering the substitution and cross‐linking of glucuronoarabinoxylans affects cell wall architecture in Brachypodium distachyon

Formation of xylem tissues and secondary cell walls is diminished by severe and consecutive insect defoliation

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