Deposition of Daldinia starbaeckii (ELF) functionalized silver nanoparticles on Urinary catheter tube using chitosan polymer to prevent bacterial biofilms formation during UTI infection

Abstract

Catheter-associated urinary tract infections (CAUTI) are the most common healthcare problem in hospitals. In this study, we isolated and the Daldinia starbaeckii (An endolichenic fungus from Roccella montagnie) and its biomass extract was used to simultaneously synthesize and deposit DSFAgNPs on the inner and outer surfaces of the catheter tube using chitosan biopolymer via In-Situ deposition method. Perfectly designed D. starbaeckii extract functionalized DSFAgNPs were characterized by UV spectroscopy, FTIR, SEM, EDS, TEM, and XRD. The microbial efficacy of DSFAgNPs & DSFAgNPs coated catheter (CTH3) was evaluated against nine human pathogenic gram (+/-) ive strains. Results indicated DSFAgNPs showed significant biological activity against both gram (+/-) ive bacteria with an average MIC of 4µl/ml. The most promising activity was observed against Helicobacter pylori. CTH3 treated bacterial strains showed a significant reduction in colony formation unit (CFU/ml) in broth culture assay. Antibiofilm activity of CTH3 showed strong inhibition of biofilm formation (85%).