Depolarization and Neurotransmitter Regulation of Vasopressin Gene Expression in the Rat Suprachiasmatic Nucleus<i>In Vitro</i>

Rusnak, Milan; Tóth, Zsuzsanna; House, Shirley B.; Gainer, Harold

doi:10.1523/jneurosci.3739-06.2007

Cited by 14 publications

(12 citation statements)

References 63 publications

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“…We found that TTX treatment results in reduced synchrony among SCN cells and that its removal allows the circadian oscillators to resynchronize, consistent with prior reports based on Per1 or Per2 gene activity and intracellular calcium levels (Yamaguchi et al 2003; Maywood et al, 2006; Aton et al, 2006; Webb et al, 2009; Ko et al, 2010; Enoki et al, 2012; Evans et al, 2013; Baba et al, 2008; Rusnak et al, 2007). During resynchronization, the period of the cells in the dorsal SCN gradually approached the period of the ventral SCN until they reached a steady state phase relationship with the ventral peaking after the dorsal SCN each day.…”

Section: Discussionsupporting

confidence: 91%

Resynchronization Dynamics Reveal that the Ventral Entrains the Dorsal Suprachiasmatic Nucleus

et al. 2016

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Although the suprachiasmatic nucleus (SCN) has long been considered the master circadian clock in mammals, the topology of the connections that synchronize daily rhythms among SCN cells is not well understood. We combined experimental and computational methods to infer the directed, interactions that mediate circadian synchrony between regions of the SCN. We analyzed PERIOD2 (PER2) expression from SCN slices during and after treatment with tetrodotoxin, allowing us to map connections as cells resynchronized their daily cycling following blockade and restoration of cell-cell communication. Using automated analyses, we found that cells in the dorsal SCN stabilized their periods slower than those in the ventral SCN. A phase-amplitude computational model of the SCN revealed that, to reproduce the experimental results: (1) the ventral SCN had to be more densely connected than the dorsal SCN and (2) the ventral SCN needed strong connections to the dorsal SCN. Taken together, these results provide direct evidence that the ventral SCN entrains the dorsal SCN in constant conditions.

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Section: Discussionsupporting

confidence: 91%

Resynchronization Dynamics Reveal that the Ventral Entrains the Dorsal Suprachiasmatic Nucleus

et al. 2016

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“…MCNs are found in the SON, PVN, and a dispersed population of MCNs called the ACC nucleus. The Avp immunopositive SCN neurons, which contain only small Avp-expressing neurons, are also found in these organotypic cultures (Rusnak et al, 2007; Wray et al, 1993). We find that most of the Oxt and Avp MCNs are typically located in one slice (as shown in Fig 1), but smaller numbers of these cells from each nucleus can be found in the three other slices from a single animal.…”

Section: Resultsmentioning

confidence: 66%

“…Certain neuronal systems in the hypothalamus such as LHRH neurons in the OVLT-MPOA region (Wray et al, 1988)and the suprachiasmatic nuclei (SCN) (Belenky et al, 1996)survive exceptionally well in organotypic cultures. As a result, the SCN in organotypic culture has been an outstanding and reliable model for the study of the generation of circadian rhythms in this nucleus (Maywood et al, 2007; Maywood et al, 2006; O’Neill et al, 2008; Rusnak et al, 2007; Tominaga et al, 1994). For other neuronal phenotypes, however, such as the magnocellular neurons (MCNs) in the hypothalamo-neurohypophysial system (HNS) it has been found that while the topography of the MCNs is maintained in organotypic culture, the survival of these neurons particularly the Avp-MCNs, the PVN and SON is very poor and highly variable (House et al, 1998; Wray et al, 1991).…”

Section: Introductionmentioning

confidence: 99%

Effects of ciliary neurotrophic factor and leukemia inhibiting factor on oxytocin and vasopressin magnocellular neuron survival in rat and mouse hypothalamic organotypic cultures

House¹,

Li²,

Yue³

et al. 2009

Journal of Neuroscience Methods

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Organotypic cultures of mouse and rat magnocellular neurons (MCNs) in the hypothalamo-neurohypophysial system (HNS) have served as important experimental models for the molecular and physiological study of this neuronal phenotype. However, it has been difficult to maintain significant numbers of the MCNs, particularly vasopressin MCNs, in these cultures for long periods. In this paper, we describe the use of the neurotrophic factors, leukemia inhibiting factor (LIF) and ciliary neurotrophic factor (CNTF) to rescue rat vasopressin (Avp)- and oxytocin (Oxt) – MCNs from axotomy-induced, programmed cell death in vitro. Quantitative data are presented for the efficacy of the LIF family of neurotrophic factors on the survival of MCNs in three nuclei, the paraventricular (PVN), supraoptic (SON), and accessory (ACC) nuclei in the mouse and rat hypothalamus.

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“…, 1998) that rhythmic release and gene expression of AVP in the SCN depend mainly on the mechanisms endogenous to the SCN. VIP was found to be the most effective ligand in evoking a cAMP‐mitogen‐activated protein kinase signal transduction cascade leading to an increase in vasopressin gene transcription in the SCN (Rusnak et al. , 2007).…”

Section: Discussionmentioning

confidence: 99%

Synaptic contacts of vesicular glutamate transporter 2 fibres on chemically identified neurons of the hypothalamic suprachiasmatic nucleus of the rat

Kiss

Csáki

Halász

2008

Eur J of Neuroscience

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The hypothalamic suprachiasmatic nucleus (SCN), which plays a pivotal role in the control of circadian rhythms, consists of several neuronal subpopulations characterized by different neuroactive substances. This prominent cell group has a fairly rich glutamatergic innervation, but the cell types that are targeted by this innervation are unknown. Therefore, the purpose of the present study was to examine the relationship between the afferent glutamatergic axon terminals and the vasoactive intestinal polypeptide (VIP)-, arginine-vasopressin (AVP)- and gamma-aminobutyric acid (GABA)-positive neurons of the SCN. Glutamatergic elements were revealed via immunocytochemical double-labelling for vesicular glutamate transporter type 1 (VGluT1) and type 2 (VGluT2), and brain sections were imaged via confocal laser-scanning microscopy and electron microscopy. Numerous VGluT2-immunoreactive axons were observed to be in synaptic contact with VIP- and GABA-positive neurons, and only a few synapses were detected between VGluT2 boutons and AVP neurons. VGluT1 axon terminals exhibiting very moderate distribution in this cell group were observed to be in synaptic contact with chemically unidentified neurons. The findings provide the first morphological data on the termination of presumed glutamatergic fibres on chemically identified neurons of the rat SCN, and indicate that all three prominent cell types of the cell group receive glutamatergic afferents.

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Depolarization and Neurotransmitter Regulation of Vasopressin Gene Expression in the Rat Suprachiasmatic NucleusIn Vitro

Cited by 14 publications

References 63 publications

Resynchronization Dynamics Reveal that the Ventral Entrains the Dorsal Suprachiasmatic Nucleus

Resynchronization Dynamics Reveal that the Ventral Entrains the Dorsal Suprachiasmatic Nucleus

Effects of ciliary neurotrophic factor and leukemia inhibiting factor on oxytocin and vasopressin magnocellular neuron survival in rat and mouse hypothalamic organotypic cultures

Synaptic contacts of vesicular glutamate transporter 2 fibres on chemically identified neurons of the hypothalamic suprachiasmatic nucleus of the rat

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