Depletion of serotonin in the basolateral amygdala elevates glutamate receptors and facilitates fear-potentiated startle

Tran, Lee; Lasher, B K; Young, Keith A.; Keele, N. Bradley

doi:10.1038/tp.2013.66

Cited by 29 publications

(33 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Stereotaxic infusions of 5,7-DHT were performed as previously described [7]. Animals were anesthetized with intraperitoneal (i.p.)…”

Section: Methodsmentioning

confidence: 99%

“…Animals recovered for two weeks prior to experimentation. Histological verification of 5,7-DHT lesion was described previously [7], and infusion locations are described in figure 1A.…”

Section: Methodsmentioning

confidence: 99%

“…Quantification of mRNA was conducted as previous described [7, 10]. Whole cell RNA was extracted using TRI Reagent (Molecular Research Center, Inc., Cincinnati, OH) following the manufacturer’s instructions.…”

Section: Methodsmentioning

confidence: 99%

“…Moreover, the mechanisms of hyperexcitability, which involve increased glutamatergic activity [2, 3], may be caused by the serotonin (5-hydroxytryptamine, 5-HT) dysfunction associated with abnormal emotional behavior [4–6]. In support, we recently showed that targeted depletion of 5-HT the rat basolateral nucleus of the amygdala (BLA) using bilateral stereotaxic infusions of the serotonergic neurotoxin 5’7-dihydroxytryptamine (DHT) increased fear potentiated startle and expression of the ionotropic glutamate receptor subunit (GluA1) [7]. Although evidence suggested low 5-HT facilitates neuronal excitability through alteration of the glutamatergic system [1, 7–10], the molecular mechanisms facilitating this process are unclear.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

CaMKIIα knockdown decreases anxiety in the open field and low serotonin-induced upregulation of GluA1 in the basolateral amygdala

Tran

Keele

2016

Behavioural Brain Research

View full text Add to dashboard Cite

Hyperactivation of the amygdala is implicated in anxiety and mood disorders, but the precise underlying mechanisms are unclear. We previously reported that depletion of serotonin (5-hydroxytryptamine, 5-HT) in the basolateral nucleus of the amygdala (BLA) using the serotonergic neurotoxin 5,7-dihydroxytryptamine (5,7-DHT) potentiated learned fear and increased glutamate receptor (Glu) expression in BLA. Here we investigated the hypothesis that CaMKII facilitates anxiety-like behavior and increased Glu/AMPA receptor subunit A1 (GluA1) expression following depletion of 5-HT in the BLA. Infusion of 5,7-DHT into the BLA resulted in anxiety-like behavior in the open field test (OFT) and increased the phosphorylation of CaMKIIα (Thr-286) in the BLA. Knockdown of the CaMKIIα subunit using adeno-associated virus (AAV)-delivered shRNAi concomitantly attenuated anxiety-like behavior in the OFT and decreased GluA1 expression in the BLA. Our results suggest that the CaMKII signaling plays a key role in low 5-HT-induced anxiety and mood disturbances, potentially through regulation of GluA1 expression in the BLA.

show abstract

“…Stereotaxic infusions of 5,7-DHT were performed as previously described [7]. Animals were anesthetized with intraperitoneal (i.p.)…”

Section: Methodsmentioning

confidence: 99%

“…Animals recovered for two weeks prior to experimentation. Histological verification of 5,7-DHT lesion was described previously [7], and infusion locations are described in figure 1A.…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

CaMKIIα knockdown decreases anxiety in the open field and low serotonin-induced upregulation of GluA1 in the basolateral amygdala

Tran

Keele

2016

Behavioural Brain Research

View full text Add to dashboard Cite

show abstract

“…54 Moreover, a recent study suggests that there may be an interaction with serotonin signaling and GluA1 levels in amygdala. 120 …”

Section: Serotoninmentioning

confidence: 99%

Molecular Mechanisms of Threat Learning in the Lateral Nucleus of the Amygdala

Sears

Schiff

LeDoux

2014

Progress in Molecular Biology and Translational Science

View full text Add to dashboard Cite

Serotonergic innervation of the human amygdala and evolutionary implications

Lew

Hanson

Groeniger

et al. 2019

American J Phys Anthropol

View full text Add to dashboard Cite

Objectives: The serotonergic system is involved in the regulation of socio-emotional behavior and heavily innervates the amygdala, a key structure of social brain circuitry.We quantified serotonergic axon density of the four major nuclei of the amygdala in humans, and examined our results in light of previously published data sets in chimpanzees and bonobos.Materials and methods: Formalin-fixed postmortem tissue sections of the amygdala from six humans were stained for serotonin transporter (SERT) utilizing immunohistochemistry. SERT-immunoreactive (ir) axon fiber density in the lateral, basal, accessory basal, and central nuclei of the amygdala was quantified using unbiased stereology.Nonparametric statistical analyses were employed to examine differences in SERT-ir axon density between amygdaloid nuclei within humans, as well as differences between humans and previously published data in chimpanzees and bonobos.Results: Humans displayed a unique pattern of serotonergic innervation of the amygdala, and SERT-ir axon density was significantly greater in the central nucleus compared to the lateral nucleus. SERT-ir axon density was significantly greater in humans compared to chimpanzees in the basal, accessory basal, and central nuclei. SERT-ir axon density was greater in humans compared to bonobos in the accessory basal and central nuclei. Conclusions:The human pattern of SERT-ir axon distribution in the amygdala complements the redistribution of neurons in the amygdala in human evolution. The present findings suggest that differential serotonergic modulation of cognitive and autonomic pathways in the amygdala in humans, bonobos, and chimpanzees may contribute to species-level differences in social behavior. K E Y W O R D Samygdala, brain evolution, serotonin, social behavior

show abstract

Depletion of serotonin in the basolateral amygdala elevates glutamate receptors and facilitates fear-potentiated startle

Cited by 29 publications

References 36 publications

CaMKIIα knockdown decreases anxiety in the open field and low serotonin-induced upregulation of GluA1 in the basolateral amygdala

CaMKIIα knockdown decreases anxiety in the open field and low serotonin-induced upregulation of GluA1 in the basolateral amygdala

Molecular Mechanisms of Threat Learning in the Lateral Nucleus of the Amygdala

Serotonergic innervation of the human amygdala and evolutionary implications

Contact Info

Product

Resources

About