Depletion of polyubiquitin encoded by the UBI4 gene confers pleiotropic phenotype to Candida albicans cells

Roig, Patricia; Gozalbo, Daniel

doi:10.1016/s1087-1845(03)00004-5

Cited by 25 publications

(39 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Overexpression of ScUBI4 complements the defects of Ddoa1 in S. cerevisiae (Johnson et al, 1995) and the orthologue of UBI4 from C. albicans can complement the defects of Dubi4 in S. cerevisiae (Roig & Gozalbo, 2003). C. albicans wild-type cells upregulate UBI4 during thermal stress and starvation, and mutants lacking Ubi4 have a moderately increased sensitivity to heat shock (Roig & Gozalbo, 2003).…”

Section: Discussionmentioning

confidence: 97%

Multiple functions of DOA1 in Candida albicans

et al. 2007

View full text Add to dashboard Cite

While searching for regulators of virulence attributes of the human-pathogenic fungus Candida albicans, a gene was identified similar to the genes encoding the mammalian phospholipase A2-activating protein (PLAP) and the Saccharomyces cerevisiae protein Doa1, which is known to play a key role during ubiquitin (Ub)-dependent protein degradation. All three proteins contain WD-repeats. Both PLAP and CaDoa1 contain a mellitin-like sequence with a central 'KVL'. This mellitin-like sequence was shown to be necessary for full function of CaDoa1. CaDOA1 was expressed under all conditions investigated. Gene disruption of CaDOA1 caused phenotypes including modified colony morphologies, temperature sensitivity, reduced secretion of hydrolytic enzymes and hypersensitivity to various compounds such as propranolol, butanol, caffeine, chelators, azoles, nocodazole and cadmium. Strikingly, mutants lacking DOA1 were filamentous and grew as pseudohyphae and true hyphae under conditions that normally support yeast growth. Transcriptional profiling of Ddoa1 indicated that several genes associated with Ub-mediated proteolysis, including CDC48 and UBI4, are upregulated. These data suggest that DOA1 of C. albicans, like its orthologue in S. cerevisiae, is associated with Ub-mediated proteolysis and has multiple functions. However, some functions of CaDoa1 seem to be unique for C. albicans. These results support the hypothesis that Ub-mediated proteolysis plays an important role in the regulation of morphology in C. albicans.

show abstract

Section: Discussionmentioning

confidence: 97%

Multiple functions of DOA1 in Candida albicans

et al. 2007

View full text Add to dashboard Cite

show abstract

“…Experiments looking for genes regulating fitness adaptability identified loss-of-function mutations in UBR1, an E3 ubiquitin ligase involved in N-end rule degradation (29), and genes involved in protein degradation suppress growth defects associated with mutational burdens presumably by stabilizing hypomorphic proteins (30). The role for ubiquitin may be as a pleiotropic regulator and UBI4 hypomorphic alleles produce spontaneous phenotypic variants in hyphal development and morphogenesis in candida (31).…”

Section: Discussionmentioning

confidence: 99%

Recurrent ubiquitin B silencing in gynecological cancers establishes dependence on ubiquitin C

Kedves¹,

Gleim²,

Liang³

et al. 2017

Journal of Clinical Investigation

View full text Add to dashboard Cite

“…Deleting grrA inhibits the formation of mature ascospores due to a block in meiosis (79). In C. albicans, the deletion of UBI4 leads to a heterogeneous mixture of yeast and hyphal cells (88,146) whereby the hyphal cells display an apparent mitotic arrest (88). The exact mechanisms by which this occurs are currently unknown.…”

Section: Reversible Peptide Modificationsmentioning

confidence: 99%

Posttranslational Modifications of Proteins in the Pathobiology of Medically Relevant Fungi

Leach

Brown

2012

Eukaryot Cell

View full text Add to dashboard Cite

Posttranslational modifications of proteins drive a wide variety of cellular processes in eukaryotes, regulating cell growth and division as well as adaptive and developmental processes. With regard to the fungal kingdom, most information about posttranslational modifications has been generated through studies of the model yeasts Saccharomyces cerevisiae and Schizosaccharomyces pombe, where, for example, the roles of protein phosphorylation, glycosylation, acetylation, ubiquitination, sumoylation, and neddylation have been dissected. More recently, information has begun to emerge for the medically important fungal pathogens Candida albicans, Aspergillus fumigatus, and Cryptococcus neoformans, highlighting the relevance of posttranslational modifications for virulence. We review the available literature on protein modifications in fungal pathogens, focusing in particular upon the reversible peptide modifications sumoylation, ubiquitination, and neddylation.

show abstract

Depletion of polyubiquitin encoded by the UBI4 gene confers pleiotropic phenotype to Candida albicans cells

Cited by 25 publications

References 41 publications

Multiple functions of DOA1 in Candida albicans

Multiple functions of DOA1 in Candida albicans

Recurrent ubiquitin B silencing in gynecological cancers establishes dependence on ubiquitin C

Posttranslational Modifications of Proteins in the Pathobiology of Medically Relevant Fungi

Contact Info

Product

Resources

About