Depletion of phosphatidylcholine affects endoplasmic reticulum morphology and protein traffic at the Golgi complex

Testerink, N.; Sanden, Michiel H.M. van der; Houweling, Martin; Helms, J. Bernd; Vaandrager, Arie B.

doi:10.1194/jlr.m800660-jlr200

Cited by 55 publications

(42 citation statements)

References 44 publications

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“…Alternatively, we regulated DAG by knocking down CTP:phosphocholine cytidylyltransferase, the regulatory enzyme for PC synthesis, for a short time. Interestingly, a temperature shift to 40°C in CHO-MT58 for longer times than those used here leads to a PC mass reduction or TAG increase (40,44). In the first case, the authors observed a decrease in secretion and suggested that PC was needed for protein transport at the Golgi.…”

Section: Discussionmentioning

confidence: 73%

Phospholipid Synthesis Participates in the Regulation of Diacylglycerol Required for Membrane Trafficking at the Golgi Complex

Sarri¹,

Sicart²,

Lázaro‐Diéguez³

et al. 2011

Journal of Biological Chemistry

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The lipid metabolite diacylglycerol (DAG) is required for transport carrier biogenesis at the Golgi, although how cells regulate its levels is not well understood. Phospholipid synthesis involves highly regulated pathways that consume DAG and can contribute to its regulation. Here we altered phosphatidylcholine (PC) and phosphatidylinositol synthesis for a short period of time in CHO cells to evaluate the changes in DAG and its effects in membrane trafficking at the Golgi. We found that cellular DAG rapidly increased when PC synthesis was inhibited at the non-permissive temperature for the rate-limiting step of PC synthesis in CHO-MT58 cells. DAG also increased when choline and inositol were not supplied. The major phospholipid classes and triacylglycerol remained unaltered for both experimental approaches. The analysis of Golgi ultrastructure and membrane trafficking showed that 1) the accumulation of the budding vesicular profiles induced by propanolol was prevented by inhibition of PC synthesis, 2) the density of KDEL receptor-containing punctated structures at the endoplasmic reticulum-Golgi interface correlated with the amount of DAG, and 3) the postGolgi transport of the yellow fluorescent temperature-sensitive G protein of stomatitis virus and the secretion of a secretory form of HRP were both reduced when DAG was lowered. We confirmed that DAG-consuming reactions of lipid synthesis were present in Golgi-enriched fractions. We conclude that phospholipid synthesis pathways play a significant role to regulate the DAG required in Golgi-dependent membrane trafficking.

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Section: Discussionmentioning

confidence: 73%

Phospholipid Synthesis Participates in the Regulation of Diacylglycerol Required for Membrane Trafficking at the Golgi Complex

Sarri¹,

Sicart²,

Lázaro‐Diéguez³

et al. 2011

Journal of Biological Chemistry

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“…Manipulation of the CDP-choline pathway with specific enzyme inhibitors, metabolites, or temperature-induced destabilization of CCTa in CHO MT-58 cells was used to vary cellular DAG content. The latter was positively correlated with modulation of vesicular transport of select proteins from Golgi to the surface membrane, but did not affect ER to Golgi or lysosome to PM traffic [9,115]. Based on the yeast secretion model systems, it was suggested that high levels of PC, induced by a PC turnover defect, trigger secretory impairment [116].…”

Section: The Role Of Cct In Vesicular Transportmentioning

confidence: 97%

CTP:phosphocholine cytidylyltransferase: Function, regulation, and structure of an amphitropic enzyme required for membrane biogenesis

Cornell

Ridgway

2015

Progress in Lipid Research

124

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“…Denuded oocytes were then washed again in PBS and fixed in 4% (v/v) paraformaldehyde (PF; Electron Microscopy Sciences, Hatfield, PA) at 378C for 1 h and stored in 1% (v/v) PF at 48C for a maximum of 1 wk. Oocytes were washed twice in PBS with 0.3% (w/v) polyvinylpyrrolidone (PVP), permeabilized for 30 min in PBS with 0.1% (w/v) saponin (PBS-S; Riedel-de Haën, Seelze, Germany) and 0.1 M glycin (Merck) and washed in PBS-S. To determine the maturational stage, oocytes were stained with 10 lg/ml TO-PRO-3 (Molecular Probes, Eugene, OR) for 20 min and subsequently three times washed in PBS-S. After this, neutral lipids in lipid droplets were stained according to a modified protocol of Chinese hamster ovary cells [30]. Lipid droplets were stained with the specific neutral lipid stain BODIPY 493/503 (Molecular Probes) in PBS (20 lg/ml, 1 h), and oocytes were washed three times in PBS with 3 mg/ml PVP.…”

Section: Lipid Droplet Staining Of Oocytesmentioning

confidence: 99%

Oleic Acid Prevents Detrimental Effects of Saturated Fatty Acids on Bovine Oocyte Developmental Competence1

Aardema

Vos

Lolicato

et al. 2011

Biology of Reproduction

Self Cite

245

221

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Mobilization of fatty acids from adipose tissue during metabolic stress will increase the amount of free fatty acids in blood and follicular fluid and, thus, may affect oocyte quality. In this in vitro study, the three predominant fatty acids in follicular fluid (saturated palmitic and stearic acid and unsaturated oleic acid) were presented to maturing oocytes to test whether fatty acids can affect lipid storage of the oocyte and developmental competence postfertilization. Palmitic and stearic acid had a dose-dependent inhibitory effect on the amount of fat stored in lipid droplets and a concomitant detrimental effect on oocyte developmental competence. Oleic acid, in contrast, had the opposite effect, causing an increase of lipid storage in lipid droplets and an improvement of oocyte developmental competence. Remarkably, the adverse effects of palmitic and stearic acid could be counteracted by oleic acid. These results suggest that the ratio and amount of saturated and unsaturated fatty acid is relevant for lipid storage in the maturing oocyte and that this relates to the developmental competence of maturing oocytes.

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Depletion of phosphatidylcholine affects endoplasmic reticulum morphology and protein traffic at the Golgi complex

Cited by 55 publications

References 44 publications

Phospholipid Synthesis Participates in the Regulation of Diacylglycerol Required for Membrane Trafficking at the Golgi Complex

Phospholipid Synthesis Participates in the Regulation of Diacylglycerol Required for Membrane Trafficking at the Golgi Complex

CTP:phosphocholine cytidylyltransferase: Function, regulation, and structure of an amphitropic enzyme required for membrane biogenesis

Oleic Acid Prevents Detrimental Effects of Saturated Fatty Acids on Bovine Oocyte Developmental Competence1

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