Depletion interactions in polymer solutions promote red blood cell adhesion to albumin-coated surfaces

Neu, Björn; Meiselman, Herbert J.

doi:10.1016/j.bbagen.2006.09.005

Cited by 24 publications

(22 citation statements)

References 19 publications

(29 reference statements)

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“…Elevated levels of fibrinogen, a symptom of a number of illness and medical conditions (including cardiovascular diseases, inflammation and pregnancy), are conjectured to lead to strong aggregation of red blood cells (RBCs; ~6.6×2 m), resulting in the rouleaux formation as shown in Fig. 4(b), as compared to weak aggregation in healthy blood [78][79][80][81]. This depletion mediated RBC aggregation process is shown schematically in Fig.…”

Section: Proteo-nanofluids Mediated Depletion In Cellular Organisatiomentioning

confidence: 91%

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Depletion forces between particles immersed in nanofluids

Briscoe

2015

Current Opinion in Colloid & Interface Science

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Section: Proteo-nanofluids Mediated Depletion In Cellular Organisatiomentioning

confidence: 91%

“…For example, dextran has been found to similarly induce rouleaux formation (Fig. 4(c) inset) [79][80][81].…”

Section: Proteo-nanofluids Mediated Depletion In Cellular Organisatiomentioning

confidence: 92%

Depletion forces between particles immersed in nanofluids

Briscoe

2015

Current Opinion in Colloid & Interface Science

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“…2,22,23,29 However, the specific mechanisms involved in RBC aggregation have not yet been fully elucidated, and two models have been proposed: (a) bridging of cells by cross-linking macromolecules 11 ; and (b) attractive forces due to osmotic gradients generated by the depletion of macromolecules in intercellular spaces. 4,24,25 The depletion model was considered for the present study. In the depletion theory, cell-cell interactions are complex processes which involve, as a first approximation, a balance between depletion attractive forces and electrostatic repulsive forces.…”

Section: Introductionmentioning

confidence: 99%

A Particle Dynamic Model of Red Blood Cell Aggregation Kinetics

et al. 2009

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To elucidate the relationship between microscopic red blood cell (RBC) interactions and macroscopic rheological behavior, we propose a two-dimensional particle model capable of mimicking the main characteristics of RBC aggregation kinetics. The mechanical model of RBCs sheared in Couette flow is based on Newton law. We assumed a hydrodynamic force to move particles, a force to describe aggregation and an elasticity force. The role of molecular mass and concentration of neutral polymers on aggregation [Neu, B., and H. J. Meiselman. Biophys. J. 83:2482-2490, 2002] could be mimicked. Specifically, it was shown that for any shear rate (SR), the mean aggregate size (MAS) grew with time until it reached a constant value, which is consistent with in vitro experiments. It was also demonstrated that we could mimic the modal relationship between MAS and SR and the occurrence of maximum aggregation at about 0.1 s(-1). As anticipated, simulations indicated that an increase in aggregation force augmented MAS. Further, augmentation of the depletion layer thickness influenced MAS only for SR close to zero, which is a new finding. To conclude, our contribution reveals that the aggregation force intensity and SR influence the steady state MAS, and that the depletion and layer thickness affect the aggregation speed.

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“…Using a relatively simple system consisting of an albumin-coated glass surface and normal RBC, both 70 and 500 kDa dextran caused increased adhesion of red cells, thus indicating the development a non-specific attractive force [26]. This finding, as well as the effect of complex plasma proteins on RBC-EC adhesion, raises an obvious question: could depletion interaction be capable of causing adhesion of red blood cells to endothelial cells (Fig.…”

Section: Red Blood Cell Adhesionmentioning

confidence: 97%

Macromolecular depletion as a determinant of RBC adhesive interactions: Why blood is thicker than water

Neu

Meiselman²

2014

Biorheology

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If a surface is in contact with a solution containing macromolecules or proteins, and the loss of configurational entropy of these molecules at the surface is not balanced by adsorption energy, a polymer-poor layer will develop near the surface. If two such layers overlap, an attractive force develops due to the osmotic pressure difference between these depletion zones and the bulk phase. Recent studies have shown that depletion interaction plays a major role in red blood cell (RBC) aggregation and hence it is a major determinate of blood flow stability; depletion interaction also markedly affects RBC adhesion to vascular endothelial cells. Understanding and quantitating factors that regulate depletion in vivo are thus of importance, yet made difficult since only very small changes of the cell surface (e.g., glycocalyx thickness) such as seen during RBC aging can lead to massive changes of depletion interaction and hence cell-cell adhesion. It is suggested that insight into the in vivo relevance of depletion mechanisms may lead to an improved understanding of how and why blood flow is altered in many diseases, and may also provide new biomarkers (e.g., surface properties) that will aid in the development of novel or improved diagnostic and therapeutic tools.

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Depletion interactions in polymer solutions promote red blood cell adhesion to albumin-coated surfaces

Cited by 24 publications

References 19 publications

Depletion forces between particles immersed in nanofluids

Depletion forces between particles immersed in nanofluids

A Particle Dynamic Model of Red Blood Cell Aggregation Kinetics

Macromolecular depletion as a determinant of RBC adhesive interactions: Why blood is thicker than water

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