Depletion effect and biomembrane budding

Liu, Qing; Chen, Yingbing; Jiang, Chongming; Li, Baike; Tang, Yan-Lin; Lin, Hai–Qing; Deng, Linhong

doi:10.1007/s10867-013-9325-9

Cited by 6 publications

(5 citation statements)

References 32 publications

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“…In this spatial configuration, the entropy of the SUVs is maximized, thus corresponding to the most thermodynamically favored structure of the system. Similar observations were previously reported for large particles, which spontaneously adsorbed at the vesicle periphery when co-encapsulated with smaller negatively charged particles. , …”

Section: Resultssupporting

confidence: 90%

“…Similar observations were previously reported for large particles, which spontaneously adsorbed at the vesicle periphery when co-encapsulated with smaller negatively charged particles. 56 , 57 …”

Section: Resultsmentioning

confidence: 99%

“…Similar observations were previously reported for large particles, which spontaneously adsorbed at the vesicle periphery when coencapsulated with smaller negatively charged particles. 56,57 ■ SUMMARY AND OUTLOOK In summary, the usage of pH as an internal or external trigger to activate the charge-mediated assembly of dsGUVs allows for the reconstruction of an endomembrane system in either a bulk assembly or by microfluidics. To achieve a successful formation of GUVs, the method relies on two important criteria: (1) slightly negatively charged SUVs can be efficiently entrapped and (2) the use of a pH-sensitive lipid.…”

Section: Acsmentioning

confidence: 99%

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pH-Triggered Assembly of Endomembrane Multicompartments in Synthetic Cells

et al. 2021

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By using electrostatic interactions as driving force to assemble vesicles, the droplet-stabilized method was recently applied to reconstitute and encapsulate proteins, or compartments, inside giant unilamellar vesicles (GUVs) to act as minimal synthetic cells. However, the droplet-stabilized approach exhibits low production efficiency associated with the troublesome release of the GUVs from the stabilized droplets, corresponding to a major hurdle for the droplet-stabilized approach. Herein, we report the use of pH as a potential trigger to self-assemble droplet-stabilized GUVs (dsGUVs) by either bulk or droplet-based microfluidics. Moreover, pH enables the generation of compartmentalized GUVs with flexibility and robustness. By co-encapsulating pH-sensitive small unilamellar vesicles (SUVs), negatively charged SUVs, and/or proteins, we show that acidification of the droplets efficiently produces dsGUVs while sequestrating the co-encapsulated material. Most importantly, the pH-mediated assembly of dsGUVs significantly improves the production efficiency of free-standing GUVs (i.e., released from the stabilizing-droplets) compared to its previous implementation.

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Section: Resultssupporting

confidence: 90%

Section: Resultsmentioning

confidence: 99%

Section: Acsmentioning

confidence: 99%

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pH-Triggered Assembly of Endomembrane Multicompartments in Synthetic Cells

et al. 2021

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Section: Ph-mediated Assembly Of Multicompartment Guvs With An Actin-cytoskeletonmentioning

confidence: 99%

pH-Triggered Assembly of Endomembrane Multicompartments in Synthetic Cells

Lussier

Schroeter

Diercks

et al. 2021

Preprint

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Bottom-up synthetic biology thrives to reconstruct basic cellular processes into a minimalist cellular replica to foster their investigation in greater details with a reduced number of variables. Among these cellular features, the endomembrane system is an important aspect of cells which is at the origin of many of their functions. Still, the reconstruction of these inner compartments within a lipid-based vesicle remains challenging and poorly controlled. Herein, we report the use of pH as external trigger to self-assemble compartmentalized giant unilamellar vesicles (GUVs) by either bulk, or droplet-based microfluidics. By co-encapsulating pH sensitive small unilamellar vesicles (SUVs), negatively charged SUVs and/or proteins, we show that acidification of the droplets efficiently produces GUVs while sequestrating the co-encapsulated material with flexibility and robustness. The method enables the simultaneous reconstruction of more than a single cellular phenotype from the bottom-up, corresponding to an important advancement in the current status quo of bottom-up synthetic biology.

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“…The biological endocytosis progress is very complicated, because there are many influences such as the concentration of the particles, the cell type, the cell environments and the physical-chemical properties of particles. Vesicles possess many similar properties as cells making them ideal model systems [5][6][7][8][9][10][11][12][13]. It is thus natural for researchers to choose the complex of a vesicle and a particle as a model system to understand the endocytotic and exocytotic processes [14][15][16][17][18][19].…”

Section: Introductionmentioning

confidence: 99%

Conformations of a charged vesicle interacting with an oppositely charged particle

Duan

Zhang

et al. 2017

J Biol Phys

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Endocytotic and exocytotic processes are usually studied using particle-vesicle systems in theory, but most of them are electroneutral. Nevertheless, charged particle-vesicle systems are much closer to real biological systems. Therefore, wrapping behaviors of a negatively charged vesicle wrapping a positively charged particle are systematically investigated by a series of 2D dynamical simulations in this article. The competition between the elastic bending energy and the electrostatic energy dictates the vesicle configuration and charge distribution. It is found that only for intermediate charge concentrations and small particle sizes a vesicle can completely engulf the particle. When the charge density is high, the interaction between vesicle and particle is unexpectedly weakened by both the hardening effect of the charged membrane and the effective-transportation-frozen effect of the charged components. When the particle is strongly charged, multi-layer folding conformations are observed. These studies may provide important insights into mechanism of endocytotic and exocytotic processes in biological systems.

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Depletion effect and biomembrane budding

Cited by 6 publications

References 32 publications

pH-Triggered Assembly of Endomembrane Multicompartments in Synthetic Cells

pH-Triggered Assembly of Endomembrane Multicompartments in Synthetic Cells

pH-Triggered Assembly of Endomembrane Multicompartments in Synthetic Cells

Conformations of a charged vesicle interacting with an oppositely charged particle

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