DEPIS: A combined dielectrophoresis and impedance spectroscopy platform for rapid cell viability and antimicrobial susceptibility analysis

Swami, Pragya; Sharma, Ayush; Anand, Satyam; Gupta, Shalini

doi:10.1016/j.bios.2021.113190

Cited by 25 publications

(14 citation statements)

References 65 publications

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“…For example, the detection or quantification of commonly used redox species is widely demonstrated, covering both inner-and outer-sphere electron transfer reactions that show sensor performance and surface sensitivity. 11,19 Biologically relevant molecules such as glucose or dopamine have also been targeted to directly showcase that FFF has the potential to produce fully personalized biosensors 20,21 with sensitivities in the range of 0.01-0.8 µmol L −1 in dopamine 22,23 and 2.4-36.4 µmol L −1 for glucose, 15,24 and in the case of glucose, ruthenium-based mediators have been employed to ease detection and improve sensitivity. [25][26][27][28][29] These 3D-printed biosensors possess a range of limitations that include: (i) their size, with electrodes up to 10 mm in diameter and several millimeters in thickness, 8,12 (ii) the associated large sample volumes that these dimensions require, 8,12,[30][31][32] and (iii) the restriction of the use of 3D-printing to produce only the working electrode, requiring the use of external counter and reference electrodes that complicate experimental setup and use.…”

Section: Introductionmentioning

confidence: 99%

Integrated multi-material portable 3D-printed platform for electrochemical detection of dopamine and glucose

Domingo-Roca

MacDonald

Hannah

et al. 2022

Analyst

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Section: Introductionmentioning

confidence: 99%

Integrated multi-material portable 3D-printed platform for electrochemical detection of dopamine and glucose

Domingo-Roca

MacDonald

Hannah

et al. 2022

Analyst

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“…Although phenotypic AST is time-consuming, it is accurate, straightforward, and suitable for any clinical sample. With the help of advanced technologies, such as electrochemical monitoring, impedance spectroscopy, Raman scattering imagination, dielectrophoresis, and impedance spectroscopy, AST can be finished within 1 h, even less than 30 min. However, specific instruments are required to perform these methods, hence limiting the wide application in hospitals.…”

Section: Introductionmentioning

confidence: 99%

Multiplexed and Rapid AST for Escherichia coli Infection by Simultaneously Pyrosequencing Multiple Barcodes Each Specific to an Antibiotic Exposed to a Sample

Feng

Yue

et al. 2022

Anal. Chem.

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Antimicrobial susceptibility testing (AST) is an effective way to guide antibiotic selection. However, conventional culture-based phenotypic AST is time-consuming. The key point to shorten the test is to quantify the small change in the bacterial number after the antibiotic exposure. To achieve rapid AST, we proposed a combination of multiplexed PCR with barcoded pyrosequencing to significantly shorten the time for antibiotic exposure. First, bacteria exposed to each antibiotic were labeled with a unique barcode. Then, the pool of the barcoded products was amplified by PCR with a universal primer pair. Finally, barcodes in the amplicons were individually and quantitatively decoded by pyrosequencing. As pyrosequencing is able to discriminate as low as 5% variation in target concentrations, as short as 7.5 min was enough for cultivation to detect the susceptibility of Escherichia coli to an antibiotic. The barcodes enable more than six kinds of drugs or six kinds of concentrations of a drug to be tested at a time. The susceptibility of 6 antibiotics to 43 E. coli-positive samples from 482 clinical urine samples showed a consistency of 99.3% for drugresistant samples and of 95.7% for drug-sensitive samples in comparison with the conventional method. In addition, the minimum inhibitory concentration (MIC) of 29 E. coli samples was successfully measured. The proposed AST is dye free (pyrosequencing), multiplexed (six antibiotics), fast (a half-working day for reporting the results), and able to detect the MIC, thus having a great potential for clinical use in quick antibiotic selection.

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“…This is a non-destructive, label-free approach allowing dynamic high-throughput assays and a continuous monitoring of the investigated biological process to identify the onset of anomalies. ECIS platforms have been shown to be able to monitor motion, attachment, growth, spread, and differentiation of cultured cells [ 7 , 8 , 9 ], quantifying cell viability and heterogeneity [ 10 , 11 ], as well as cell migration and invasive activities [ 12 , 13 , 14 ], and evaluating the effects of biochemical compounds and cytotoxicity [ 15 , 16 , 17 , 18 ]. Recently, close attention has been devoted to applications for drug research/screening [ 11 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 ].…”

Section: Introductionmentioning

confidence: 99%

“…ECIS platforms have been shown to be able to monitor motion, attachment, growth, spread, and differentiation of cultured cells [ 7 , 8 , 9 ], quantifying cell viability and heterogeneity [ 10 , 11 ], as well as cell migration and invasive activities [ 12 , 13 , 14 ], and evaluating the effects of biochemical compounds and cytotoxicity [ 15 , 16 , 17 , 18 ]. Recently, close attention has been devoted to applications for drug research/screening [ 11 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 ]. In our study, a highly reliable platform for ECIS cell proliferation and drug screening assays is presented, validated by assessing the efficacy of Sorafenib for the treatment of HCC.…”

Section: Introductionmentioning

confidence: 99%

Validation of a Lab-on-Chip Assay for Measuring Sorafenib Effectiveness on HCC Cell Proliferation

Piccinno

Monteduro

Dituri

et al. 2021

IJMS

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Hepatocellular carcinoma (HCC) is a highly lethal cancer, and although a few drugs are available for treatment, therapeutic effectiveness is still unsatisfactory. New drugs are urgently needed for hepatocellular carcinoma (HCC) patients. In this context, reliable preclinical assays are of paramount importance to screen the effectiveness of new drugs and, in particular, measure their effects on HCC cell proliferation. However, cell proliferation measurement is a time-consuming and operator-dependent procedure. The aim of this study was to validate an engineered miniaturized on-chip platform for real-time, non-destructive cell proliferation assays and drug screening. The effectiveness of Sorafenib, the first-line drug mainly used for patients with advanced HCC, was tested in parallel, comparing the gold standard 96-well-plate assay and our new lab-on-chip platform. Results from the lab-on-chip are consistent in intra-assay replicates and comparable to the output of standard crystal violet proliferation assays for assessing Sorafenib effectiveness on HCC cell proliferation. The miniaturized platform presents several advantages in terms of lesser reagents consumption, operator time, and costs, as well as overcoming a number of technical and operator-dependent pitfalls. Moreover, the number of cells required is lower, a relevant issue when primary cell cultures are used. In conclusion, the availability of inexpensive on-chip assays can speed up drug development, especially by using patient-derived samples to take into account disease heterogeneity and patient-specific characteristics.

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DEPIS: A combined dielectrophoresis and impedance spectroscopy platform for rapid cell viability and antimicrobial susceptibility analysis

Cited by 25 publications

References 65 publications

Integrated multi-material portable 3D-printed platform for electrochemical detection of dopamine and glucose

Integrated multi-material portable 3D-printed platform for electrochemical detection of dopamine and glucose

Multiplexed and Rapid AST for Escherichia coli Infection by Simultaneously Pyrosequencing Multiple Barcodes Each Specific to an Antibiotic Exposed to a Sample

Validation of a Lab-on-Chip Assay for Measuring Sorafenib Effectiveness on HCC Cell Proliferation

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