2017
DOI: 10.1080/10942912.2016.1261151
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Dephytinization of food stuffs by phytase of Geobacillus sp. TF16 immobilized in chitosan and calcium-alginate

Abstract: In this work, Geobacillus sp. TF16 phytase was separately immobilized in chitosan and Ca-alginate with the efficiency of 38% and 42%, respectively. These enzymes exhibited broad substrate specificity. Maximal relative phytase activity was measured at pH 5.0 and 95°C and pH 3.0 and 75°C for chitosan and Ca-alginate, respectively. The enzymes were highly stable in a wide pH and temperature range. Values of K m and V max were determined as 2.38 mM and 3401.36 U/mg protein for chitosan, and 7.5 mM and 5011.12 U/mg… Show more

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Cited by 11 publications
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“…TF-16 for soy milk phytate was calculated as 24 and 33%, respectively. The chitosan-and Ca-alginate immobilized enzymes conserved their original activity after 8 and 6 cycles of reuse, respectively [114].…”
Section: Production and Applications Of Thermostable Enzymes Of Geobamentioning
confidence: 99%
“…TF-16 for soy milk phytate was calculated as 24 and 33%, respectively. The chitosan-and Ca-alginate immobilized enzymes conserved their original activity after 8 and 6 cycles of reuse, respectively [114].…”
Section: Production and Applications Of Thermostable Enzymes Of Geobamentioning
confidence: 99%