Dephenolization and decolorization of olive mill wastewater through sequential batch and co-culture applications

Abstract

In a previous work it was reported adapted Trametes versicolor FPRL 28A INI culture was used to treat undiluted olive mill wastewater (OMW) without addition of any nutrients with significant amount of total phenolics were removed. However, decolorization was not so pronounced. Therefore, the aim of this study is to enhance the efficiency of dephenolization and decolorization of the primary treatment with adapted Trametes versicolor, incorporating a secondary biological treatment step using different microorganisms with sequential batch and co-culture applications. Through sequential batch applications Funalia trogii ATCC 20080 was found to have a higher potential in terms of total phenolics removal and decolorization amongst the tested organisms and better results were obtained from sequential batch applications as compared to co-culture experiments. In sequential batch applications, up to 91% total phenolics were removed and 64% decolorization was achieved after 24 days with 20% (v/v) inoculation rate of F. trogii when malt extract broth was used in inoculum preparation. In addition, significant accumulation of laccase (2019 ± 121.13Ul -1 ) and manganese peroxidase (463 ± 33.89 Ul -1 ) activities were attained. In co-culture applications highest total phenolics removal and decolorization were 78 and 39%, respectively, with non-adapted T. versicolor, whereas highest laccase and manganese peroxidase acitivities were obtained with F. trogii as 2219 Ul -1 ± 176.14 . and 513 ± 4.12 Ul -1 , respectively.

Production of Added-Value Chemical Compounds through Bioconversions of Olive-Mill Wastewaters Blended with Crude Glycerol by a Yarrowia lipolytica Strain

“…Specifically, in all cases, Y. lipolytica strain ACA-DC 5029 presented the ability to remove phenolic compounds up to ~10% w / w (~0.4 g/L maximum removal of phenols in culture with Ph 0 ~3.5 g/L) and color up to ~30%, compared to media composition prior to fermentation (Figure 2). This is a crucial result as it has been reported that the limiting step of OMW remediation is the breakdown of its phenolic compounds (and therefore the removal of OMW color) [1,3,4,12]. This fact could be attributed to potential adsorption of phenolic compounds in the yeast cells (through weak and reversible interactions, mainly between anthocyanins and yeast walls) [48] or even to partial utilization of phenolic compounds as carbon and energy source [49].…”

“…It is considered as one of the most challenging agro-industrial wastes to treat [1]. OMW has dark color, strong odor and (phyto-)toxic properties, mainly attributed to high quantities of phenolic compounds that it contains [2,3,4,5,6]. OMW is produced in vast volumes, seasonally and geographically scattered [7] and is characterized by extremely high biological (BOD) and chemical oxygen demand (COD) (values up to 200–400 times higher than typical municipal sewage) [1].…”

Decolorization of Industrial Dyes by Immobilized Mycelia ofTrametes Versicolor

“…hence, the biodegradation abilities of basidiomycete fungi as well as of other fungi are subject of intensive investigations. the main focus of these studies is the ability of fungi to remove synthetic dyes in industrial wastewater by enzymatic biodegradation or mineralization (4,19,21,23,24,28,29,33,34 (8,18) so there is increased interest in the application of immobilized filamentous fungi in different processes of biodegradation of pollutants. Researchers have focused on the immobilization of basidiomycetes, which are known as efficient producers of laccase and peroxidase (1,7,9,10,26) in order to develop effective systems for detoxification and decolorization of wastewater.…”

Firing Range Soils Yield a Diverse Array of Fungal Isolates Capable of Organic Acid Production and Pb Mineral Solubilization

“…Oxalic acid production in white-rot fungi is thought to aid lignocellulose depolymerization by producing radical species, buffering the microenvironment of the fungus, facilitating oxidative enzyme activity, and aiding metal chelation (11) and tolerance (17). P. flavidoalba has not specifically been reported to produce LMWOAs but has been studied for bioremediation of olive mill wastewater through decolorization and depolymerization of various toxic components in wastewater streams (13,32,45). T. versicolor has also been studied for the same purpose (12), as well as for bioremediation of polycyclic aromatic hydrocarbons (46).…”