Dependence of the Rate of DNA Synthesis in X-Irradiated HeLa S3 Cells on Dose and Time after Exposure

Tolmach, L. J.; Jones, R. W.

doi:10.2307/3574522

Cited by 30 publications

(6 citation statements)

References 9 publications

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“…In contrast, no inhibition of DNA synthesis was observed in cells exposed to a concentration of MMS (200 pglml for 30 min) which produced a frequency of pH 13.0-detectable lesions equivalent to that in cells heated at 45°C for 60 min. Maximum inhibition of 3H-thymidine incorporation was not expressed immediately after exposure to X-radiation, but, as reported previously (Tolmach and Jones, 1977), was expressed 90 min after X-irradiation (Fig. 4).…”

Section: Resultssupporting

confidence: 86%

Apurinic site induction in the DNA of cells heated at hyperthermic temperatures

Warters

Brizgys

1987

Journal Cellular Physiology

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The induction of DNA damage in cells heated at hyperthermic (43-48 degrees C) temperatures was determined by alkaline filter elution and alkaline sucrose gradient-sedimentation analysis of cell DNA denatured at pH 13.0. A class of DNA lesion which converted to strand breaks during denaturation of DNA at pH 13.0 was produced randomly throughout the cell DNA at temperatures as low as 43 degrees C. Induction of this lesion occurred with a T0 of 90 and 10 min at 45 and 48 degrees C, respectively. We estimate that these pH 13.0-detectable DNA lesions are produced in the cell DNA with a frequency of approximately 75 and 660 per min of heating at 45 and 48 degrees C, respectively. Since the lesions were quantitatively converted to DNA strand breaks at pH 13.0 with a half-time of 30 min, or less, we suggest that these pH 13.0-detectable DNA lesions are heat-induced, abasic DNA sites. The induction of these lesions does not appear to be directly involved in the initial heat-induced inhibition of DNA synthesis. The presence of these lesions cannot be excluded as an explanation for the long-term inhibition of replicon initiated in heated cells.

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Section: Resultssupporting

confidence: 86%

Apurinic site induction in the DNA of cells heated at hyperthermic temperatures

Warters

Brizgys

1987

Journal Cellular Physiology

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“…1965) ; (3) a temporary block of replicon initiation (Wantanable 1974, Makino and Okada 1975, Tolmach and Jones 1977 ; (4) a competitive inhibition of exogenous thymidine incorporation by DNA breakdown products (Smets and Brohee 1970) ; (5) damage to DNA associated structures (Okada 1970, Weiss 1971 . It is also known that cell membranes are very sensitive to irradiation (Koteles 1982, Yatvin et al 1980) .…”

Section: 6 the Effects Of Irradiationmentioning

confidence: 98%

“…01 Gy have been reported to reduce significantly the incorporation of 3 HTdR (Feinendegen et al 1984) . Due to the complex chain of reactions leading to the incorporation of precursors into DNA, the inhibition of incorporation is strongly dependent on the cell system and the conditions in the experiments (Smets and Brohee 1970, Painter 1967, Watanabe 1974, Tolmach and Jones 1977, Okada 1970, Zamboglou et al 1981 .…”

Section: Introductionmentioning

confidence: 98%

Influence of Irradiation or Thymidine (TdR) on the Pattern of³H-TdR Incorporation at Each Cell Position in the Crypts of the Small Intestine of the Mouse

Chwalinski

Potten

1987

International Journal of Radiation Biology and Related Studies

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Using autoradiographic methods it was noted that S phase cells at the bottom of the crypts in the small intestine were the most efficient scavengers of exogenous injected thymidine. The efficiency of the incorporation of 3H-TdR (salvage pathway of DNA synthesis) by cells at the crypt base (stem cell zone) was twice as high as for the S phase cells at the top of the crypt (maturing proliferative cells). There were no such position-dependent differences in incorporation of 3H-UdR (de novo pathway of DNA synthesis). Radiation (0.75-5.0 Gy 137Cs gamma-rays) inhibited the incorporation of 3H-TdR very rapidly and this was also cell-position dependent. The cells at the bottom of the crypt were the most affected. The injection of cold thymidine before 3H-TdR changed the pattern of the incorporation of 3H-TdR along the side of the crypt in a very similar way to radiation, and the grain number was decreased predominantly in the cells at lower positions. The possibility of the existence of a regional gradient of endogenous thymidine (reutilization from intestinal sources), and the influence of irradiation on the gradient of thymidine incorporation resulting from direct and abscopal effects of whole body exposure, are discussed.

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“…Thereafter and up to 2.5 Gy/min no changes could be observed. Dose rate effects 'have been reported by MARTIN (1971, 1977) and STRANGE & MURPHREE (1972. It is assumed that the main critical injury to the cells was the introduction of DNA injury.…”

Section: Discussionmentioning

confidence: 97%

Effects of Low Doses of Gamma Radiation on DNA Synthesis in the Developing Rat Brain

Cerda¹

1983

Acta Radiologica: Oncology

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Dependence of the Rate of DNA Synthesis in X-Irradiated HeLa S3 Cells on Dose and Time after Exposure

Cited by 30 publications

References 9 publications

Apurinic site induction in the DNA of cells heated at hyperthermic temperatures

Apurinic site induction in the DNA of cells heated at hyperthermic temperatures

Influence of Irradiation or Thymidine (TdR) on the Pattern of³H-TdR Incorporation at Each Cell Position in the Crypts of the Small Intestine of the Mouse

Effects of Low Doses of Gamma Radiation on DNA Synthesis in the Developing Rat Brain

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Dependence of the Rate of DNA Synthesis in X-Irradiated HeLa S3 Cells on Dose and Time after Exposure

Cited by 30 publications

References 9 publications

Apurinic site induction in the DNA of cells heated at hyperthermic temperatures

Apurinic site induction in the DNA of cells heated at hyperthermic temperatures

Influence of Irradiation or Thymidine (TdR) on the Pattern of3H-TdR Incorporation at Each Cell Position in the Crypts of the Small Intestine of the Mouse

Effects of Low Doses of Gamma Radiation on DNA Synthesis in the Developing Rat Brain

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Influence of Irradiation or Thymidine (TdR) on the Pattern of³H-TdR Incorporation at Each Cell Position in the Crypts of the Small Intestine of the Mouse