Dependence of reversed-phase retention of ionizable analytes on pH, concentration of organic solvent and silanol activity

Comparison of separation techniques for the elucidation of IgG N-glycans pooled from healthy mammalian species

“…Extensive research efforts have been put into the understanding of 520 the fundamental retention behaviour of analytes when separated by columns comprised of various packing materials; in particular the separation by monolithic silica, 42,43 or silanol silica. 44 For the analysis of larger complex glycans RP separation would be most efficient especially when coupled with mass spectrometry. 9,41,45,46 Such coupling however, increases analysis times and requires further expertise.…”

Tris(2-methyl-8-quinolinolato)gallium(III) as a Fluorescent Probe for Sensitive Silanol-Testing

“…10,[17][18][19][20][21][22] Here, we compared the peak parameters of the gallium(III) complex of the present work with the silanol activities of E, 17,18 G, 20 ; silica gel, 80 -100 mesh (column chromatography grade). Fig.…”

“…5,7,[10][11][12][13][14][15][16] Most of the probe compounds are small molecules containing nitrogen atoms, because much interest has been focused on the interaction through the ion-exchange or hydrogen-bonding function to which nitrogen-containing compounds are considered to respond. 10,[17][18][19][20][21][22][23] Claessens et al 16,24,25 examined the relations between various silanol-detecting molecules containing nitrogen atoms, and found a correlation among the results of the tests. They concluded that the silanol activity was influenced by individual factors on the surface of silica-base packing materials, such as deprotonation of the silanol groups, the existence of highly acidic silanol and metal impurities, and that each silanol test evaluated the degree of silanol activity differently.…”

Triclopyr 3, 5, 6-Trichloro-2-Pyridinyl Clean-Up Procedure from Soil, Sediment and Water Samples using SPE-HPLC-VWD

“…Solid phase extraction (SPE) is popular and most commonly used among all the clean-up procedures. The sorbent is vital to acquire high efficiency in solid phase extraction technique (Neue et al 2001). For the determination of triclopyr in water samples, additional set up was required in sample preparation procedure.…”