Dependence of in vitro Enzymic Hydrolysis of Alkyl(2-benzoyloxyethyl)dimethylammonium Bromides on the Alkyl Length

Abstract

Microsomal esterases were used in the study of the in vitro enzymic hydrolysis of the ester bond in alkyl(2-benzoyloxyethyl)dimethylammonium bromides. These compounds are potential "soft" disinfectants, easily biodegradable to nontoxic biologically inactive hydrolytic products, namely substituted choline and benzoic acid. Formation of the latter product was used to monitor the kinetics of the reaction. It has been found that the rate of enzymic hydrolysis is substantially influenced by different length of the alkyl chain on the ammonium nitrogen. At the same time, interspecies (rat-mouse) and interorgan (liver-kidney) variability has been observed.

Inter-tissue and inter-species comparison of butyrylcholinesterases

“…While rabbit microsomal BuChE activity increased, rat microsomal BuChE activity decreased as a function of alkyl chain length of BCHn. Increasing BuChE activity has already been reported for the mouse kidney microsomal fraction (Olaszová et al, 1998). The course of BuChE activity towards BCHn in mouse kidney was the same as that for rabbit microsomal fraction.…”

“…Low BuChE activity for BCH 10 can also be explained by production of aggregated forms. Micelle formation could decrease an actual concentration of substrate available for enzyme (Olaszová et al, 1998). For longest-chain substrates, BCH 12 -BCH 18 , the non-linear progress curve v versus t (v-t curves) was observed.…”

Biodegradable gemini surfactants. Correlation of area per surfactant molecule with surfactant structure

“…Such compounds are non-toxic and can be safely removed from the living organisms. The rate of in vitro hydrolysis of these surfactants was studied in liver and lung of rats and its dependence on surfactant alkyl chain length was examined [1][2][3].…”