Dependence of dopamine calibration factors on media Ca2+ and Mg2+ at carbon-fiber microelectrodes used with fast-scan cyclic voltammetry

Kume-Kick, June; Rice, Margaret E.

doi:10.1016/s0165-0270(98)00087-9

Cited by 44 publications

(48 citation statements)

References 28 publications

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“…Ca 2+ in the extracellular space is reported to reduce by 0.1 mM after intense local stimulation in slice. 18,21,22 The decrease in Ca 2+ concentration from 0.1 to 0.0 mM induced intense current changes at FSCV and t′0−5. However, it was much smaller in the physiological concentration change from 1.1 mM to 1.0 mM ( Figure 5) and in ACSF (see Figures S6C and S6D in the Supporting Information, from 1.25 mM to 1.15 mM).…”

Section: ■ Resultsmentioning

confidence: 99%

“…The dopamine response in ACSF was smaller than that in PBS at FSCV (0.73 ± 0.08, n = 3) and t5−25 (0.70 ± 0.01), the result of FSCV is consistent with previous studies. 22 . 23 Unlike dopamine, it was difficult to determine the change in pH sensitivity for FSCV in ACSF, because of the marked change in differential waveform of pH response (S6G).…”

Section: ■ Resultsmentioning

confidence: 99%

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Temporal Differentiation of pH-Dependent Capacitive Current from Dopamine

Yoshimi

Weitemier

2014

Anal. Chem.

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Voltammetric recording of dopamine (DA) with fast-scan cyclic voltammetry (FSCV) on carbon fiber microelectrodes have been widely used, because of its high sensitivity to dopamine. However, since an electric double layer on a carbon fiber surface in a physiological ionic solution behaves as a capacitor, fast voltage manipulation in FSCV induces large capacitive current. The faradic current from oxidation/reduction of target chemicals must be extracted from this large background current. It is known that ionic shifts, including H(+), influence this capacitance, and pH shift can cause confounding influences on the FSCV recordings within a wide range of voltage. Besides FSCV with a triangular waveform, we have been using rectangular pulse voltammetry (RPV) for dopamine detection in the brain. In this method, the onset of a single pulse causes a large capacitive current, but unlike FSCV, the capacitive current is restricted to a narrow temporal window of just after pulse onset (<5 ms). In contrast, the peak of faradic current from dopamine oxidation occurs after a delay of more than a few milliseconds. Taking advantage of the temporal difference, we show that RPV could distinguish dopamine from pH shifts clearly and easily. In addition, the early onset current was useful to evaluate pH shifts. The narrow voltage window of our RPV pulse allowed a clear differentiation of dopamine and serotonin (5-HT), as we have shown previously. Additional recording with RPV, alongside FSCV, would improve identification of chemicals such as dopamine, pH, and 5-HT.

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Section: ■ Resultsmentioning

confidence: 99%

Section: ■ Resultsmentioning

confidence: 99%

Temporal Differentiation of pH-Dependent Capacitive Current from Dopamine

Yoshimi

Weitemier

2014

Anal. Chem.

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“…Voltage scanning parameters [11], electrode conditioning [33] and environmental factors limit in vivo accuracy based upon flow cell electrode calibration. Environmental factors affecting current response to intracerebral DA include local calcium (Ca 2+ ) and magnesium (Mg 2+ ) concentrations [34], interference by pH and O 2 changes [18], fouling of the carbon fiber surface by extracellular metabolites [35] and the difference between flow-cell temperatures and body temperatures [36].…”

Section: Methodsmentioning

confidence: 99%

Real-Time Dopamine Measurement in Awake Monkeys

et al. 2014

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Fast-scan cyclic voltammetry (FSCV) is often used to measure real-time dopamine (DA) concentrations in awake, behaving rodents. Extending this technique to work in monkeys would provide a platform for advanced behavioral studies and a primate model for preclinical research. The present study demonstrates the feasibility of DA recordings in two awake monkeys (Macaca mulatta) using a mixture of techniques adapted from rodent, primate and brain slice work. We developed a long carbon fiber electrode to operate in the larger primate brain. This electrode was lowered into the striatum each day using a recording chamber and a detachable micromanipulator system. A manipulator also moved one or more tungsten stimulating electrodes into either the nearby striatum or the ventral tegmental area/substantia nigra pars compacta (VTA/SNc). We developed an electrical stimulation controller to reduce artifacts during electrical stimulation. We also introduce a stimulation-based methodology for estimating distances between electrodes in the brain. Dopamine responses within the striatum were evoked by either stimulation of the striatum near the FSCV electrode, or stimulation within the VTA/SNc. Unexpected juice rewards also evoked dopamine responses in the ventral striatum. Thus, we demonstrate that robust dopamine responses can be recorded from awake, behaving primates with FSCV. In addition, we describe how a stimulation technique borrowed from the neuroprosthetics field can activate the distributed monkey midbrain dopamine system in a way that mimics rodent VTA stimulation.

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“…Rice and co-workers found that the FCV sensitivities to DA and 5-HT at CFEs were 2-3-fold higher in non-physiological phosphate or HEPES-buffered saline compared to artificial cerebrospinal fluid (aCSF) which more accurately reflects the ionic composition of the brain (Kume-Kick and Rice, 1998;Chen and Rice, 1999). Interestingly, the reverse was observed for the sensitivities of the acid metabolites (DOPAC, 5-HIAA).…”

Section: Effect Of Ion Changesmentioning

confidence: 99%

“…ca. 80 M for 10 Hz at 10 s) (Kume-Kick and Rice, 1998) and intense tissue depolarisation (e.g. >1 mM for anoxic depolarisation or spreading depression) (Nicholson and Rice, 1988), we decided to test the effect of ion changes (Ca 2+ and Mg 2+ ) on the CPE O 2 response in aCSF.…”

Section: Effect Of Ion Changesmentioning

confidence: 99%