Dependence of Chs2 ER export on dephosphorylation by cytoplasmic Cdc14 ensures that septum formation follows mitosis

Chin, Cheen Fei; Bennett, Alexis M.; Kit, Wai; Hall, Mark C.; Yeong, Foong May

doi:10.1091/mbc.e11-05-0434

Cited by 63 publications

(93 citation statements)

References 80 publications

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“…The mitotic kinase Cdk1 likely acts directly on Chs2, which contains four CDK1 phosphorylation sites near its N terminus, because mutation of the target Ser residues to Glu leads to retention of Chs2 in the ER, whereas changing the serines to Ala leads to constitutive release of the mutant Chs2 even in the presence of high Cdk1 activity (Teh et al 2009). Timed release of Chs2 from the ER after chromosome separation and exit of the cells from mitosis is triggered by dephosphorylation of the Cdk1 sites by the Cdc14 phosphatase, the terminal component of the mitotic exit network (MEN) cascade (Chin et al 2012).…”

Section: Biosynthesis Of Wall Components At the Plasma Membranementioning

confidence: 99%

Architecture and Biosynthesis of the Saccharomyces cerevisiae Cell Wall

2012

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The wall gives a Saccharomyces cerevisiae cell its osmotic integrity; defines cell shape during budding growth, mating, sporulation, and pseudohypha formation; and presents adhesive glycoproteins to other yeast cells. The wall consists of b1,3-and b1,6-glucans, a small amount of chitin, and many different proteins that may bear N-and O-linked glycans and a glycolipid anchor. These components become cross-linked in various ways to form higher-order complexes. Wall composition and degree of cross-linking vary during growth and development and change in response to cell wall stress. This article reviews wall biogenesis in vegetative cells, covering the structure of wall components and how they are cross-linked; the biosynthesis of N-and O-linked glycans, glycosylphosphatidylinositol membrane anchors, b1,3-and b1,6-linked glucans, and chitin; the reactions that cross-link wall components; and the possible functions of enzymatic and nonenzymatic cell wall proteins. TABLE OF CONTENTS Abstract 775Introduction 777

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Section: Biosynthesis Of Wall Components At the Plasma Membranementioning

confidence: 99%

Architecture and Biosynthesis of the Saccharomyces cerevisiae Cell Wall

2012

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“…Chs2's entry into the secretory system is antagonized by phosphorylation of CDK sites in its N terminus (Teh et al 2009), and Cdc14 reverses this modification to allow trafficking of Chs2, the site of primary septum formation (Chin et al 2012). Intriguingly, while CDK phosphorylation likely blocks Chs2's secretion, it also greatly stabilizes the protein.…”

Section: Regulation Of Septation Machinery and The Cytokinetic Apparatusmentioning

confidence: 99%

Mitotic Exit and Separation of Mother and Daughter Cells

2012

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Productive cell proliferation involves efficient and accurate splitting of the dividing cell into two separate entities. This orderly process reflects coordination of diverse cytological events by regulatory systems that drive the cell from mitosis into G1. In the budding yeast Saccharomyces cerevisiae, separation of mother and daughter cells involves coordinated actomyosin ring contraction and septum synthesis, followed by septum destruction. These events occur in precise and rapid sequence once chromosomes are segregated and are linked with spindle organization and mitotic progress by intricate cell cycle control machinery. Additionally, critical parts of the mother/daughter separation process are asymmetric, reflecting a form of fate specification that occurs in every cell division. This chapter describes central events of budding yeast cell separation, as well as the control pathways that integrate them and link them with the cell cycle.

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“…Dephosphorylation of Cdk1 substrates underlies many events of late mitosis and cytokinesis (Bloom et al, 2011;Chin et al, 2012;Khmelinskii et al, 2009;Mishima et al, 2004;Sullivan et al, 2008;Teh et al, 2009). Cdc14 localizes to the bud neck prior to the onset of cytokinesis and is known to promote primary septum deposition (Palani et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Cdk1-dependent phosphorylation of Iqg1 governs actomyosin ring assembly prior to cytokinesis

Naylor

Morgan

2014

Journal of Cell Science

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Contraction of the actomyosin ring (AMR) provides the centripetal force that drives cytokinesis. In budding yeast (Saccharomyces cerevisiae), assembly and contraction of the AMR is coordinated with membrane deposition and septum formation at the bud neck. A central player in this process is Iqg1, which promotes recruitment of actin to the myosin ring and links AMR assembly with that of septum-forming components. We observed early actin recruitment in response to inhibition of cyclin-dependent kinase 1 (Cdk1) activity, and we find that the Cdk1-dependent phosphorylation state of Iqg1 is a determining factor in the timing of bud neck localization of both Iqg1 and actin, with both proteins accumulating prematurely in cells expressing nonphosphorylatable Iqg1 mutants. We also identified the primary septum regulator Hof1 as a binding partner of Iqg1, providing a regulatory link between the septation and contractile pathways that cooperate to complete cytokinesis.

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Dependence of Chs2 ER export on dephosphorylation by cytoplasmic Cdc14 ensures that septum formation follows mitosis

Abstract: Sequestration of Cdc14 from the cytoplasm ensures Chs2 ER retention after MEN activation. The interdependence of chromosome segregation, MEN activation, decrease in mitotic CDK activity, and Cdc14 dispersal provides an effective mechanism for cells to order late mitotic events.

Cited by 63 publications

References 80 publications

Architecture and Biosynthesis of the Saccharomyces cerevisiae Cell Wall

Architecture and Biosynthesis of the Saccharomyces cerevisiae Cell Wall

Mitotic Exit and Separation of Mother and Daughter Cells

Cdk1-dependent phosphorylation of Iqg1 governs actomyosin ring assembly prior to cytokinesis

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