Dependence of cardiac trabeculation on neuregulin signaling and blood flow in zebrafish

Peshkovsky, Courtney; Totong, Ronald; Yelon, Deborah

doi:10.1002/dvdy.22614

Cited by 4 publications

(12 citation statements)

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“…Extensive study of intracardiac fluid forces during cardiovascular development has demonstrated important roles in valvulogenesis and trabeculation [1,2,37,38]. Our results suggest that cardiogenesis is also regulated by extracardiac flow forces generated in the pericardial cavity.…”

Section: Discussionmentioning

confidence: 62%

“…As early as the heart tube stage, the heart begins to beat and puts the blood into motion. This generates hemodynamic forces that feed back to the heart, promoting its further maturation through valve formation and myocardial trabeculation [1,2]. At this stage, the myocardium is lined on its lumenal surface by the endocardium, but it is not yet covered by the epicardium, the mesothelial outer layer of the heart.…”

Section: Introductionmentioning

confidence: 99%

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Heartbeat-Driven Pericardiac Fluid Forces Contribute to Epicardium Morphogenesis

et al. 2013

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Section: Discussionmentioning

confidence: 62%

Section: Introductionmentioning

confidence: 99%

Heartbeat-Driven Pericardiac Fluid Forces Contribute to Epicardium Morphogenesis

et al. 2013

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“…In support of this idea, neuregulin and notch1b are both expressed in the AV endocardium, and morpholino knockdown of either of these genes inhibits AV delay. Trabecular formation is also dependent on the presence of the endocardium and requires signaling through the neuregulin coreceptor ErbB2 cell-autonomously within the myocardium, demonstrating that some ventricular conduction components rely on the same signals as the AVC myocardium (64,83). Interestingly, different components of the conduction system respond differently to cardiac function.…”

Section: Conduction Systemmentioning

confidence: 95%

“…Also around this time, myocardial sheets known as trabeculae begin to form in the developing ventricle (64,83). In other species, the fast conduction system is thought to form initially within these trabeculae (90,104).…”

Section: Conduction Systemmentioning

confidence: 99%

Uncovering the Molecular and Cellular Mechanisms of Heart Development Using the Zebrafish

2012

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Over the past 20 years, the zebrafish has emerged as a powerful model organism for studying cardiac development. Its ability to survive without an active circulation and amenability to forward genetics has led to the identification of numerous mutants whose study has helped elucidate new mechanisms in cardiac development. Furthermore, its transparent, externally developing embryos have allowed detailed cellular analyses of heart development. In this review, we discuss the molecular and cellular processes involved in zebrafish heart development from progenitor specification to development of the valve and the conduction system. We focus on imaging studies that have uncovered the cellular bases of heart development and on zebrafish mutants with cardiac abnormalities whose study has revealed novel molecular pathways in cardiac cell specification and tissue morphogenesis.

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“…First, cardiomyocytes extend protrusions luminally over their neighbors, and then gradually constrict abluminally while moving their cell body into the trabecular network (Staudt et al, 2014). Neuregulin/ErbB signaling and an optimal blood flow through the ventricle are important for the initiation and advancement of trabeculation (Peshkovsky et al, 2011). This phase is associated with diminished external growth and a switch to internal growth creating numerous trabeculations forming a complex network of lacunae classically referred to as spongy myocardium.…”

Section: Changes In Structure and Anatomymentioning

confidence: 99%

Temporal cohesion of the structural, functional and molecular characteristics of the developing zebrafish heart

et al. 2015

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Heart formation is a complex, dynamic and highly coordinated process of molecular, morphogenetic and functional factors with each interacting and contributing to formation of the mature organ. Cardiac abnormalities in early life can be lethal in mammals but not in the zebrafish embryo which has been widely used to study the developing heart. While early cardiac development in the zebrafish has been well characterized, functional changes during development and how these relate to architectural, cellular and molecular aspects of development have not been well described previously. To address this we have carefully characterised cardiac structure, function, cardiomyocyte proliferation and cardiac-specific gene expression between 48 and 120 hpf in the zebrafish. We show that the zebrafish heart increases in volume and changes shape significantly between 48 and 72 hpf accompanied by a 40% increase in cardiomyocyte number. Between 96 and 120 hpf, while external heart expansion slows, there is rapid formation of a mature and extensive trabecular network within the ventricle chamber. While ejection fraction does not change during the course of development other determinants of contractile function increase significantly particularly between 72 and 96 hpf leading to an increase in cardinal vein blood flow. This study has revealed a number of novel aspects of cardiac developmental dynamics with striking temporal orchestration of structure and function within the first few days of development. These changes are associated with changes in expression of developmental and maturational genes. This study provides important insights into the complex temporal relationship between structure and function of the developing zebrafish heart.

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Dependence of cardiac trabeculation on neuregulin signaling and blood flow in zebrafish

Cited by 4 publications

References 0 publications

Heartbeat-Driven Pericardiac Fluid Forces Contribute to Epicardium Morphogenesis

Heartbeat-Driven Pericardiac Fluid Forces Contribute to Epicardium Morphogenesis

Uncovering the Molecular and Cellular Mechanisms of Heart Development Using the Zebrafish

Temporal cohesion of the structural, functional and molecular characteristics of the developing zebrafish heart

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