Deoxyribonucleic Acid Homologies Among Coryneform Bacteria

Suzuki, Ken‐ichiro; Kaneko, Tadaaki; Komagata, Kazuo

doi:10.1099/00207713-31-2-131

“…A DNA-DNA hybridization experiment was carried out by using the membrane filter method (28). Labeled DNA was prepared by using deoxy[l' ,2',5-3H]cytidine 5'-triphosphate (TRK 625; Amersham International plc, Amersham, United Kingdom) with a nick translation kit (N. 5000; Amersham International plc), and hybridization was carried out at 70°C for 40 h. The amount of hybridized [3H]DNA was measured with a liquid scintillation counter (model LS-7800; Beckman Instruments Inc., Fullerton, Calif.).…”

Section: Methodsmentioning

confidence: 99%

Nocardia seriolae sp. nov. Causing Nocardiosis of Cultured Fish

Kudo¹,

Hatai²,

Seino³

1988

International Journal of Systematic Bacteriology

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were studied to establish their taxonomic status. Well-developed fragmenting vegetative mycelium was observed. The chemotype was type IVA containing rneso-diaminopimelic acid, arabinose, and galactose. The predominant isoprenoid quinone was MK-8(H4), and mycolic acids were present. These morphological and chemical properties are characteristic of the genus Nocurdiu. The physiological and biochemical characteristics were most similar to those of Nocurdiu usteroides; however, these organisms were different in their decomposition of urea, growth temperature, utilization of nitrogen sources, survival at 50°C for 8 h, mycolic and fatty acid profiles, and deoxyribonucleic acid-deoxyribonucleic acid hybridization characteristics. Therefore, we propose a new species for these strains, Nocurdiu seriolae. The type strain is strain JCM 3360.A disease caused by actinomycetes in cultured fishes occurred first in rudderfishes (Seriola quinqueradiata and Seriola purpurascens) in Mie Prefecture, Japan, in 1967 (2, 13) and then spread to fish farms in the western district of Japan. This disease is characterized by the formation of abscesses in the epidermis and of tubercles in gills, kidneys, and spleens (13,15,17, 18). In 1968, the causative organisms were initially isolated, and their morphological, physiological, and biochemical characteristics were examined by Kariya et al. (13). Although these authors suggested that the strains were related to Nocardia asteroides, they proposed a new species, "Nocardia kampachi," for these organisms because of differences in some physiological and biochemical characteristics. However, morphology was the sole characteristic used for assignment of these strains to the genus Nocardia, and the description of " N . kampachi" lacked designation of a type strain. This name has never been validated, and original strains are not available anymore. Later, in 1973 and1974, Kusuda and Taki (17) and Kusuda et al. (18) isolated bacteria from infected yellowtails and identified them as " N . kampachi."We studied one of the strains of Kusuda (17) and four strains newly isolated from infected fishes (yellowtails [S. quinqueradiata] and a Japanese flounder [Paralichthys olivaceus]) to establish the taxonomic status of these strains. Based on morphological and chemical properties, we assigned the isolates to the genus Nocardia, and we propose a new species, Nocardia seriolae, for them on the basis of deoxyribonucleic acid (DNA)-DNA hybridization results and mycolic and fatty acid profiles in addition to physiological and biochemical characteristics. MATERIALS AND METHODSBacterial strains. In this study we used five strains isolated from cultured fishes from various fish farms in Japan and, for comparison, some strains of N . asteroides and related strains (Table 1).Morphology. The morphology of the isolates was studied by growing the strains on dilute yeast extract-malt extract agar (ISP medium no. 2 diluted to a 1/10 concentration) at 28°C for 5 days. A sample for electron microscopy was * Corresponding au...

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“…After denaturation of the DNA, 10 pg of unlabeled DNA was immobilized on a nitrocellulose membrane (type HAWP; pore size, 0.45 pm; Millipore, Bedford, Mass. ), and DNA-DNA hybridization experiments were performed by the membrane filter method, as described by Suzuki et al (27).…”

Section: Int J Syst Bacteriolmentioning

confidence: 99%

Sulfolobus hakonensis sp. nov., a Novel Species of Acidothermophilic Archaeon

Takayanagi

¹

,

Kawasaki

²

,

Sugimori

³

et al. 1996

International Journal of Systematic Bacteriology

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“…+, Positive; W, weakly positive; 2, negative. Suzuki et al (1981), Collins (1982), Collins et al (1982), Goodfellow et al (1982), and Collins & Cummins (1986) but no formal proposal was published. [C. hoagii] was proposed earlier (Morse, 1912) , 1923).…”

mentioning

confidence: 99%

Rhodococcus defluvii sp. nov., isolated from wastewater of a bioreactor and formal proposal to reclassify [Corynebacterium hoagii] and Rhodococcus equi as Rhodococcus hoagii comb. nov.

Kämpfer

¹

,

Dott

²

,

Martin

³

et al. 2014

International Journal of Systematic and Evolutionary Microbiology

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A Gram-stain-positive, non-endospore-forming rod-shaped bacterium, strain Ca11T, was isolated from a bioreactor with extensive phosphorus removal and was studied in detail for its taxonomic allocation. 16S rRNA gene sequence analysis revealed closest sequence similarity of the strain to type strains of [ Corynebacterium hoagii ] and Rhodococcus equi (98.9 %), Rhodococcus koreensis and Rhodococcus wratislaviensis (both 98.4 %), Rhodococcus opacus and Rhodococcus canchipurensis (both 98.0 %) followed by Rhodococcus kunmingensis and Rhodococcus imtechensis (97.7 %). Phylogenetic trees showed a distinct clustering of strain Ca11T with the type strains of [ C. hoagii ], R. equi , and R. kunmingensis separate to all other species of the genus Rhodococcus . The quinone system of strain Ca11T was composed of dihydrogenated menaquinones with 8 (major amount) as well as 7 and 6 isoprenoid units [MK-8(H2), MK-7(H2), MK-6(H2)]. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannoside, one unknown phospholipid and an unidentified glycolipid. The fatty acid profile was similar to that reported for R. equi and contained major amounts of C16 : 0, C18 : 1ω9c and 10-methyl C18 : 0, supporting the allocation of the strain to the genus Rhodococcus . Physiological and biochemical characterization and DNA–DNA hybridization with type strains of the most closely related species allowed clear phenotypic and genotypic differentiation of the isolate. On the basis of these results, strain Ca11T ( = DSM 45893T = LMG 27563T) represents a novel species of the genus Rhodococcus , with the proposed name Rhodococcus defluvii sp. nov. In addition, a polyphasic taxonomic analysis of [ Corynebacterium hoagii ] DSM 20295T and Rhodococcus equi DSM 20307T indicated that the two strains belong to the same species, for which the name Rhodococcus hoagii comb. nov. takes priority, according to the Rules of the Bacteriological Code.

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Deoxyribonucleic Acid Homologies Among Coryneform Bacteria

Cited by 62 publications

References 23 publications

Nocardia seriolae sp. nov. Causing Nocardiosis of Cultured Fish

Nocardia seriolae sp. nov. Causing Nocardiosis of Cultured Fish

Sulfolobus hakonensis sp. nov., a Novel Species of Acidothermophilic Archaeon

Rhodococcus defluvii sp. nov., isolated from wastewater of a bioreactor and formal proposal to reclassify [Corynebacterium hoagii] and Rhodococcus equi as Rhodococcus hoagii comb. nov.

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