Deoxyribonucleic Acid Antibody: A Method to Detect Its Primary Interaction with Deoxyribonucleic Acid

Wold, Richard T.; Young, Frank E.; Tan, Eng M.; Farr, Richard S.

doi:10.1126/science.161.3843.806

Cited by 348 publications

(87 citation statements)

References 9 publications

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“…The Farr assay (25). Twenty-five microliters of sera, diluted 1 : 10 with borate buffered saline (BBS) (0.03M borate, 0.15M NaCl, pH 7.8), were added to 15 ng of 3H-dsDNA in 75 pl of BBS.…”

Section: Methodsmentioning

confidence: 99%

A longitudinal study of high and low avidity antibodies to double‐stranded DNA in systemic lupus erythematosus

McGrath

Biundo

1985

Arthritis & Rheumatism

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The role of avidity in the pathogenicity of doublestranded DNAIanti-double-stranded DNA immune complexes in systemic lupus erythematosus (SLE) has been controversial. We used polyethylene glycol to identify low avidity antibodies and the standard Farr assay to detect high avidity antibodies against doublestranded DNA in a longitudinal study of sera from 19 patients with SLE. We found that high and low avidity antibodies to double-stranded DNA did not move independently, but instead, rose and fell in a parallel and relatively fixed manner in these patients. The mechanisms responsible for the changes in titer of anti-doublestranded DNA antibody appeared nondiscriminatory in regard to avidity. In addition, the humoral immune response in SLE depicted by these antibody measurements appeared atypical, lacking maturational features.

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“…The Farr assay (25). Twenty-five microliters of sera, diluted 1 : 10 with borate buffered saline (BBS) (0.03M borate, 0.15M NaCl, pH 7.8), were added to 15 ng of 3H-dsDNA in 75 pl of BBS.…”

Section: Methodsmentioning

confidence: 99%

A longitudinal study of high and low avidity antibodies to double‐stranded DNA in systemic lupus erythematosus

McGrath

Biundo

1985

Arthritis & Rheumatism

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“…DNA-binding capacity of the serum of individual mice was measured by a micromodification of the technique described by Wold et al (20,21). (Table 1).…”

Section: Methodsmentioning

confidence: 99%

Influence of dietary restriction on immunologic function and renal disease in (NZB × NZW) F ₁ mice

Fernandes

Friend

Yunis

et al. 1978

Proc. Natl. Acad. Sci. U.S.A.

154

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In (NZB X NZW)F1 (B/W) mice, moderate caloric intake [10 kcal (41.8 kJ) per day] from the time of weaning was associated with maintenance of lower body weight, greater capacity of spleen cells to be stimulated with T-cel mitogens, and better preserved capacity to generate cytotoxic cells in response to in vitro and in vivo stimulation with allogeneic tumor cells. Plaque-forming cell response to sheep erythrocytes was also well maintained in animals on the restricted diets when sensitization was accomplished either in vitro or in vivo.Spontaneous suppressor cell activity against plaque-forming cells that developed in controls did not appear in the mice on the restricted diet. Significantly less circulating antibody to native DNA was present in the blood of mice 10 months of age when their dietary intake had been restricted. Histological analysis revealed that the development of renal disease and the deposition of gamma globulin in the glomerular capillaries was markedly inhibited in the mice on restricted diets. Dietary restriction from the time of weaning thus appears to prolong significantly the life of autoisnmunity-prone (NZB X NZW)F1 male and female mice and to alter lymphoid cell immune function, thereby decreasing the autoimmune processes and immunological assault associated with progressive renal disease in these animals.Over the past several years, we have analyzed the influence of diet on immunologic functions in mice, rats, and guinea pigs (1). Investigations with the autoimmunity-susceptible NZB strain of mice revealed that diets relatively high in fat and low in protein and fiber accelerated development of autoimmune disease and shortened life-span. Conversely, diets high in protein and fiber and low in fat delayed development of autoimmunity and prolonged life-span (2, 3). Furthermore, chronic protein restriction with normal calorie intake from the time of weaning in NZB mice interfered with the loss of T-cell-mediated immune functions and decreased hypergammaglobulinemia, thymic involution, and splenomegaly (4). The lowprotein diets also retarded moderately the development of autoimmune disease but did not prolong life of NZB mice (4). Relatively short-term, chronic, moderate protein restriction with normal calorie intake in rats (5), mice (6), and guinea pigs (7), inhibited antibody production but permitted expression of T-cell-mediated immunity. Extreme dietary protein deficiency inhibited both production of antibody and expression of Tcell-mediated immunities. Similar influences were obtained with chronic single amino acid restrictions (8). Walford et al. (9) showed that, in long-lived strains of mice, calorie restriction early in life prolonged immunologic vigor as well as survival.We have recently reported that dietary restriction, initiated both from the time of weaning and at an adult age, prolongs significantly the life-span of short-lived, autoimmunity-prone (NZB X NZW)F1 (B/W) mice (10, 11). Dubois et al. (12)

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“…Double-stranded calf thymus DNA and double-stranded fungal RNAt were externally labeled with tritiated actinomycin D and a modified Farr technique employed (16,17). Based on a series of determinations on adult sera, the thresholds of positivity for these methods in this laboratory had been set at 10% for DNA binding and 35% for RNA binding.…”

Section: Methodsmentioning

confidence: 99%

The association of antinuclear antibodies with the chronic iridocyclitis of juvenile rheumatoid arthritis (Still's disease)

Schaller

Johnson

Holborow

et al. 1974

Arthritis & Rheumatism

134

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Positive tests for antinuclear antibodies (ANA) were found in 51 of 58 (88%) patients with chronic iridocyclitis and juvenile rheumatoid arthritis (Still's disease). Antinuclear antibodies were predominantly of the IgG class of immunoglobulins and were generally present in titers of 1 5 0 (six ANA units) or more. They were unassociated with disease activity, severity or duration, age of patient at onset or testing, or sex. Neither other autoantibodies nor antibodies reactive with DNA or RNA were associated. In 8 patients tested early in disease, ANA were found prior to the onset of iridocyclitis. The presence of ANA should prove useful in identifying patients with JRA at risk for chronic iridocyclitis. In contrast, negative tests for ANA in patients with childhood-onset arthritis and iridocyclitis were found to be associated with acute iridocyclitis and subsequent ankylosing spondylitis.Iridocyclitis, inflammation of the iris and ciliary body, affects about 10% of children with juvenile rheumatoid arthritis (JRA,

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Deoxyribonucleic Acid Antibody: A Method to Detect Its Primary Interaction with Deoxyribonucleic Acid

Cited by 348 publications

References 9 publications

A longitudinal study of high and low avidity antibodies to double‐stranded DNA in systemic lupus erythematosus

A longitudinal study of high and low avidity antibodies to double‐stranded DNA in systemic lupus erythematosus

Influence of dietary restriction on immunologic function and renal disease in (NZB × NZW) F ₁ mice

The association of antinuclear antibodies with the chronic iridocyclitis of juvenile rheumatoid arthritis (Still's disease)

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Deoxyribonucleic Acid Antibody: A Method to Detect Its Primary Interaction with Deoxyribonucleic Acid

Cited by 348 publications

References 9 publications

A longitudinal study of high and low avidity antibodies to double‐stranded DNA in systemic lupus erythematosus

A longitudinal study of high and low avidity antibodies to double‐stranded DNA in systemic lupus erythematosus

Influence of dietary restriction on immunologic function and renal disease in (NZB × NZW) F 1 mice

The association of antinuclear antibodies with the chronic iridocyclitis of juvenile rheumatoid arthritis (Still's disease)

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Influence of dietary restriction on immunologic function and renal disease in (NZB × NZW) F ₁ mice