Deoxyribonucleic acid amplification and hybridisation in Crohn's disease using a chlamydial plasmid probe.

Mcgarity, B. H.; Robertson, Douglas R.; Clarke, Ian N.; Wright, Ralph

doi:10.1136/gut.32.9.1011

Cited by 12 publications

(12 citation statements)

References 28 publications

(7 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We believe that this is the first study in which the presence of C. pneumoniae in the human intestinal mucosa has been systematically examined although the possibility that Chlamydia , particularly C. trachomatis, might play an important role in human IBD has been considered from time to time in the past 12,20 . The finding of intestinal Chlamydia in pigs 32 provided the impetus to search for Chlamydia in the intestine of patients with IBD.…”

Section: Discussionmentioning

confidence: 97%

“…Several groups have in the past investigated the presence of Chlamydiae organisms, mainly C. trachomatis , in patients with IBD 11,12 . Antibodies against Chlamydia of the lymphogranuloma‐venereum type were detected in 69% of 55 patients with Crohn's disease, while only two of 21 patients with other gastrointestinal (GI) disorders and one of 50 healthy blood donors had weak antibody responses 13 .…”

Section: Introductionmentioning

confidence: 99%

“…reported that 93% of 15 patients with Crohn's disease had IgG antibodies against C. trachomatis , compared to 26% in the control group 14 . Nevertheless, the overall reported detection rate of serum antibodies specific for C. trachomatis in patients with IBD was low (15–20%) and similar to the detection rate in non‐IBD control subjects (30%) 12,15–18 . Recently, serology studies failed to find evidence of participation by C. psittaci in Crohn's disease 19 .…”

Section: Introductionmentioning

confidence: 99%

“…Recently, serology studies failed to find evidence of participation by C. psittaci in Crohn's disease 19 . Furthermore, several groups have failed to identify Chlamydiae antigens or specific DNA in tissues from patients with IBD using either immunohistochemical or molecular biology methods 11,12 , 18,20 . However, to the best of our knowledge, the presence of C. pneumoniae in the intestinal biopsies from patients with IBD and normal individuals has never been investigated.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Detection of Chlamydia pneumoniae by polymerase chain reaction–enzyme immunoassay in intestinal mucosal biopsies from patients with inflammatory bowel disease and controls

Chen

Liu

Wilson

et al. 2002

J of Gastro and Hepatol

View full text Add to dashboard Cite

Background and Aim : It has been suggested that Chlamydia is an organism that may have the potential to cause inflammatory bowel disease (IBD) in susceptible individuals. Chlamydia pneumoniae has emerged as an important human pathogen in the last decade. The objective of the present study was to investigate the frequency of the presence of C. pneumoniae DNA in intestinal biopsies from patients with IBD and from non-IBD controls. Methods : The DNA was extracted from 222 colonoscopic biopsies, which were obtained from 11 patients with Crohn's disease (CD), 18 patients with ulcerative colitis (UC) and from 37 non-IBD control patients. The presence of the C. pneumoniae omp1 gene and C. trachomatis 16S rRNA gene was determined using a rapid and sensitive polymerase chain reaction-enzyme immunoassay (PCR-EIA).Results : The C. pneumoniae -specific DNA was detected in 32 (14.4%) of 222 endoscopic biopsies. Among them, C. pneumoniae DNA were found in nine of 42 (21.4%) biopsies from patients with CD, nine of 59 (15.3%) biopsies from patients with UC, and 14 out of 122 (11.4%) biopsies from non-IBD control patients, respectively. Moreover, the percentage of patients with at least one biopsy positive for C. pneumoniae was higher, although not statistically significant, in CD (36.4%) and UC patients (38.9%) compared to non-IBD controls (16.2%). In contrast, C. trachomatis DNA was detected in only two of 222 (0.9%) biopsy samples. Conclusion : The C. pneumoniae DNA was detected in the intestine of both patients with IBD and in non-IBD control patients, probably reflecting the high prevalence of this organism in the environment. The moderate yield of positive biopsies in our IBD patients and the fact that the detection rate of C. pneumoniae DNA was similar in endoscopic biopsies from IBD patients and non-IBD controls does not support a direct role for this organism in the pathogenesis of IBD.

show abstract

Section: Discussionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Detection of Chlamydia pneumoniae by polymerase chain reaction–enzyme immunoassay in intestinal mucosal biopsies from patients with inflammatory bowel disease and controls

Chen

Liu

Wilson

et al. 2002

J of Gastro and Hepatol

View full text Add to dashboard Cite

show abstract

“…and Crohn's disease. McGarity et al, using a chlamydia plasma probe and PCR, found no evidence of C. trachomatis in 5 resection specimens and 5 biopsies from CD patients [16]. Employing a PCR-EIA method for the 16S rRNA gene of C. trachomatis on mucosal biopsies Chen et al found one positive colon in 11CD patients and one positive colon in 37 controls [17].…”

Section: Discussionmentioning

confidence: 99%

Evidence for Chlamydia in Crohns Disease

Kruiningen

Hayes²,

Garmendia³

et al. 2016

Int J Dig Dis

View full text Add to dashboard Cite

Chlamydia cause enteritis in young pigs and calves, and granulomas within intestinal lymphatics. Recently we called attention to the lymphangitis and lymphatic obstruction that occurs in Crohn's disease. We searched resected tissues from patients for evidence of chlamydia and sera for evidence of previous exposure to chlamydia. Immunohistochemistry and real-time PCR were employed to seek chlamydia in preserved tissues. In the IHC, antibody to C. trachomatis served as primary antibody; in the PCR, primers specific for Chlamydiaceae were employed. Commercial ELISA kits measured anti-chlamydia IgG and IgA against C. trachomatis antigen in sera derived from a population of patients different from that which yielded the tissue specimens. IHC revealed focal positive staining for chlamydia in tissues of 5 of 19 patients. Positive reacting cells occurred within dense inflammation, in sparsely scattered macrophages in the submucosa and subserosa. Tissues from 3 of 22 control subjects were positive. Real-time PCR done on ileal, colonic, and regional lymph node tissues revealed evidence of chlamydia in 3 of 33 patients. Serology for anti-chlamydia IgG revealed 2 positive values in 24 patients, while serology for anti-chlamydia IgA revealed 4 positives among the 24 patients, and 1 positive in the 15 controls. One patient and one control had both elevated IgG and IgA titers. The 4 patients with elevated IgA titers were from a single family of 6 with Crohn's disease, which had been previously described. Additional consideration needs to be given to the chlamydia species, including those of animal origin, which leave behind little evidence of their previous involvement.

show abstract

The intestinal microflora and inflammatory bowel disease

Chadwick¹,

Chen²

1999

Medical Importance of the Normal Microflora

View full text Add to dashboard Cite

Deoxyribonucleic acid amplification and hybridisation in Crohn's disease using a chlamydial plasmid probe.

Abstract: The possibility that Crohn's disease is caused by infection with Chlamydia trachomatis was examined by probing for chlamydial plasmid deoxyribonucleic acid (DNA) in DNA extracts

Cited by 12 publications

References 28 publications

Detection of Chlamydia pneumoniae by polymerase chain reaction–enzyme immunoassay in intestinal mucosal biopsies from patients with inflammatory bowel disease and controls

Detection of Chlamydia pneumoniae by polymerase chain reaction–enzyme immunoassay in intestinal mucosal biopsies from patients with inflammatory bowel disease and controls

Evidence for Chlamydia in Crohns Disease

The intestinal microflora and inflammatory bowel disease

Contact Info

Product

Resources

About