Deoxyribonuclease immobilized on the erythrocytes

Kitao, Takeshi; Kitao, Yasuko; Hattori, Kenichi

doi:10.1007/bf01986127

Cited by 3 publications

(3 citation statements)

References 3 publications

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“…An alternative target is the MvfR protein itself, which belongs to the LysR family of transcriptional regulators (LTTRs) 84,85 . LTTR proteins generally possess a highly conserved DNA-binding domain but a poorly conserved ligand binding domain (LBD).…”

Section: Main Druggable Quorum Sensing Regulators From P Aeruginosamentioning

confidence: 99%

“…LTTR proteins generally possess a highly conserved DNA-binding domain but a poorly conserved ligand binding domain (LBD). Luckily, the LBD of MvfR has been solved both in apo form and in complex with several ligands 79,85 . The X-ray structure of MvfR LBD in complex with NHQ reveals that the substrate occupies two different pockets: the quinolone pocket is composed of Tyr258, Ile186, Val170, Leu189 and Ile236 and the alkyl-chain pocket is lined by Leu207, Leu208, Ile236, Ile149, Ala168 and Phe221.…”

Section: Main Druggable Quorum Sensing Regulators From P Aeruginosamentioning

confidence: 99%

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Structure-based Druggability Assessment of Anti-virulence Targets from Pseudomonas aeruginosa

Froes

Baldini

Vajda

et al. 2019

CPPS

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Antimicrobial Resistance (AMR) represents a serious threat to health and the global economy. However, interest in antibacterial drug development has decreased substantially in recent decades. Meanwhile, anti-virulence drug development has emerged as an attractive alternative to fight AMR. Although several macromolecular targets have been explored for this goal, their druggability is a vital piece of information that has been overlooked. This review explores this subject by showing how structure- based freely available in silico tools, such as PockDrug and FTMap, might be useful for designing novel inhibitors of the pyocyanin biosynthesis pathway and improving the potency/selectivity of compounds that target the Pseudomonas aeruginosa quorum sensing mechanism. The information provided by hotspot analysis, along with binding site features, reveals novel druggable targets (PhzA and PhzS) that remain largely unexplored. However, it also highlights that in silico druggability prediction tools have several limitations that might be overcome in the near future. Meanwhile, anti-virulence drug targets should be assessed by complementary methods, such as the combined use of FTMap/PockDrug, once the consensus druggability classification reduces the risk of wasting resources on undruggable proteins.

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Section: Main Druggable Quorum Sensing Regulators From P Aeruginosamentioning

confidence: 99%

Section: Main Druggable Quorum Sensing Regulators From P Aeruginosamentioning

confidence: 99%

Structure-based Druggability Assessment of Anti-virulence Targets from Pseudomonas aeruginosa

Froes

Baldini

Vajda

et al. 2019

CPPS

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“…Therefore, the mixtures can be tailored to have higher power/energy densities, enhanced stability and lower cost [1][2][3][4][5][6][7] . One successful example is the mixture of spinel LiMn 2 O 4 (LMO) 8 and layered LiNi 1/3 Co 1/3 Mn 1/3 O 2 (NCM) 9,10 . The spinel LMO component can contribute advantages to low cost, good thermal stability even at charged state, and high electronic and ionic conductivities, but suffers from low capacity.…”

Section: Introductionmentioning

confidence: 99%

Thermal stability in the blended lithium manganese oxide – Lithium nickel cobalt manganese oxide cathode materials: An in situ time-resolved X-Ray diffraction and mass spectroscopy study

Bak

Senanayake

et al. 2015

Journal of Power Sources

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Thermal stabilities of a series of blended LiMn 2 O 4 (LMO)-LiNi 1/3 Co 1/3 Mn 1/3 O 2 (NCM) cathode materials with different weight ratios were studied by in situ time-resolved x-ray diffraction (XRD) combined with mass spectroscopy in the temperature range of 25°C to 580°C under helium atmosphere. Upon heating, the electrochemically delithiated LMO changed into Mn 3 O 4 phase at around 250°C. Formation of MnO with rock-salt structure started at 520°C. This observation is in contrast to the previous report for chemically delithiated LMO in air, in which a process of λ-MnO 2 transforming to β-MnO 2 was observed. Oxygen peak was not observed in all cases, presumably as a result of either consumption by the carbon or detection limit. CO 2 profile correlates well with the phase transition and indirectly suggests the oxygen release of the cathode. Introducing NCM into LMO has two effects: first, it makes the high temperature rock-salt phase formation more complicated with more peaks in CO 2 profile due to different MO (M=Ni, Mn, Co) phases; secondly, the onset temperature of CO 2 release is lowered, implying lowered oxygen

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Immobilized Nucleases

Reddy

Shankar

1993

Critical Reviews in Biotechnology

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Nucleases are important analytical enzymes and are used widely for the determination of nucleic acid structure. Their application depends on the specificity and mode of action of the particular enzyme. Nucleases have also found application in the production of flavor enhancers like 5' IMP and 5' GMP, removal of nucleic acids in single cell protein preparations, and as therapeutic agents. Immobilization of nucleases and the use of immobilized nucleases for various biotechnological applications have been reviewed.

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Deoxyribonuclease immobilized on the erythrocytes

Cited by 3 publications

References 3 publications

Structure-based Druggability Assessment of Anti-virulence Targets from Pseudomonas aeruginosa

Structure-based Druggability Assessment of Anti-virulence Targets from Pseudomonas aeruginosa

Thermal stability in the blended lithium manganese oxide – Lithium nickel cobalt manganese oxide cathode materials: An in situ time-resolved X-Ray diffraction and mass spectroscopy study

Immobilized Nucleases

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