Deoxynivalenol in Lebensmitteln

Curtui, Valeriu; Brockmeyer, Andreas; Dietrich, Richard; Kappenstein, Oliver; Klaffke, H.; Lepschy, J.; Märtlbauer, Erwin; Schneider, Elisabeth; Seidler, C.; Thielert, G.; Usleber, Ewald; Weber, Rudolf; Wolff, J.

doi:10.1007/bf02954424

Cited by 14 publications

(6 citation statements)

References 7 publications

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“…With respect to the relevance (frequency of exposure of humans), T-2 toxin, HT-2 toxin, DON, and NIV were selected because of their worldwide occurrence in food (Abramson et al 1998;Curtui et al 1998Curtui et al , 2005. Macrocyclic trichothecenes, satratoxin G and H, were included in this study because they are possibly involved in the aetiology of illnesses related to water-damagedbuildings (Croft et al 1986;Sorenson et al 1987; Table 1 Johanning et al 1996;Johanning et al 1998Johanning et al , 1999Gottschalk et al 2006;Pestka et al 2008).…”

Section: Resultsmentioning

confidence: 99%

Trichothecene-induced cytotoxicity on human cell lines

et al. 2009

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Trichothecene cytotoxicity of type A (T-2 toxin and HT-2 toxin), type B (deoxynivalenol, DON, and nivalenol, NIV), and type D (satratoxins G and H) compounds was determined comparatively by using eight permanent human cell lines (Hep-G2, A549, CaCo-2, HEp-2, A204, U937, RPMI 8226, and Jurkat). Viability of cells was measured by a water-soluble tetrazolium (WST-1) reagent cell proliferation assay assessing mitochondrial metabolic activity. Toxicity was expressed as the toxin concentration inhibiting 50% of cell viability (IC50). Depending on the chemotype of the tested trichothecenes, relative cytotoxic activity differed by a factor of 100-1,000, and the corresponding IC50 values were in the range from 2.2 nmol/l (satratoxin H on Jurkat and U937 cells) to 4,900 nmol/l (deoxynivalenol on HEp-2 cells). In contrast, the specific toxicity of each individual mycotoxin towards different cell lines was within remarkable close limits, and between-cell line differences were much smaller than previously reported. For the cell lines tested, IC50 values were 4.4-10.8 nmol/l for T-2 toxin, 7.5-55.8 mol/l for HT-2 toxin, 600-4,900 nmol/l for DON, 300-2,600 nmol/l for NIV, and 2.2-18.3 nmol/l for satratoxins G/H. In addition, for the first time, the toxic activity of trichothecenes on primary cell culture of human endothelial cells (HUVEC) was tested. The susceptibility of this cell line was comparable to the other cell lines tested, with IC50 values ranging from 16.5 nmol/l (T-2 toxin) to 4,500 nmol/l (DON). The results suggest that the current focus of cytotoxicological studies on trichothecenes on lymphoid cell lines may lead to an underestimate of their potential on other target cell systems.

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Section: Resultsmentioning

confidence: 99%

Trichothecene-induced cytotoxicity on human cell lines

et al. 2009

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“…Furthermore, DON and ZEA are stable under varying environmental conditions, causing a diversity of toxic effects in humans, farm animals, and laboratory animals [ 11 , 12 ]. DON, also known as vomitoxin [ 2 , 13 , 14 ], is directly transported to the brain after consumption, where it disturbs and destroys dopaminergic receptors [ 15 , 16 ]. DON is also able to resist high temperatures and can even be transferred from the animal to its products and, therefore, is present even in heat-treated products, such as milk and cheese [ 17 ].…”

Section: Introductionmentioning

confidence: 99%

Development of a LC–MS/MS Method for the Simultaneous Determination of the Mycotoxins Deoxynivalenol (DON) and Zearalenone (ZEA) in Soil Matrix

Kappenberg

Juraschek

2021

Toxins

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Mycotoxins, toxins of fungal origin, can directly or indirectly contaminate food and feed and are poisonous to livestock and humans. While a large amount is known about their occurrence in crops, food, and feeds, little is known about mycotoxin amounts in soil. However, soil is known as a major fungal habitat and a potential sink for mycotoxins in the environment. Furthermore, there is neither a reliable detection nor an extraction method for mycotoxins testing in different soil textures or for potential deficits due to aging processes. Therefore, the aim of the present study was to present a reliable extraction and detection method for the simultaneous quantification of the most common mycotoxins, deoxynivalenol (DON) and zearalenone (ZEA), via liquid chromatography-tandem mass spectrometry (LC–MS/MS). This method was validated with six different samples with different textures and different soil organic matter (SOM). Deuterated standards were used to overcome possible matrix effects. This extraction method could eliminate potential aging processes. The recovery rate was always >80% for DON and >82% for ZEA. The quantification limits were 1 ng per g soil for DON and 0.5 ng per g soil for ZEA.

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“…The occurrence of different mycotoxins in beer has been reported earlier, with most studies focusing on deoxynivalenol (1; DON), mainly due to its high incidence. 5,6 Another mycotoxin, ochratoxin A (5; OTA), although detected in beer at low concentrations (only <0.2 ng/mL), 7,8 is of interest due to its high toxicity. According to EU legislation, the maximum levels (MLs) relevant for barley are set to be 1250 μg/kg for DON (in unprocessed cereals other than durum wheat, oats, and maize) and 5 μg/kg for OTA (in unprocessed cereals).…”

Section: ■ Introductionmentioning

confidence: 99%

Analysis of Mycotoxins in Beer Using a Portable Nanostructured Imaging Surface Plasmon Resonance Biosensor

Joshi

Annida

Zuilhof

et al. 2016

J. Agric. Food Chem.

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A competitive inhibition immunoassay is described for the mycotoxins deoxynivalenol (DON) and ochratoxin A (OTA) in beer using a portable nanostructured imaging surface plasmon resonance (iSPR) biosensor, also referred to as imaging nanoplasmonics. The toxins were directly and covalently immobilized on a 3-dimensional carboxymethylated dextran (CMD) layer on a nanostructured iSPR chip. The assay is based on competition between the immobilized mycotoxins and free mycotoxins in the solution for binding to specific antibodies. The chip surface was regenerated after each cycle, and the combination of CMD and direct immobilization of toxins allowed the chips to be used for more than 450 cycles. The limits of detection (LODs) in beer were 17 ng/mL for DON and 7 ng/mL for OTA (or 0.09 ng/mL after 75 times enrichment). These LODs allowed detection of even less than 10% depletion of the tolerable daily intake of DON and OTA by beer. Significant cross-reactivity of anti-DON was observed toward DON-3-glucoside and 3-acetyl-DON, while no cross-reactivity was seen for 15-acetyl-DON. A preliminary in-house validation with 20 different batches of beer showed that both toxins can be detected at the considered theoretical safe level for beer. The assay can be used for in-field or at-line detection of DON in beer and also in barley without preconcentration, while OTA in beer requires an additional enrichment step, thus making the latter in its present form less suitable for field applications.

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Deoxynivalenol in Lebensmitteln

Cited by 14 publications

References 7 publications

Trichothecene-induced cytotoxicity on human cell lines

Trichothecene-induced cytotoxicity on human cell lines

Development of a LC–MS/MS Method for the Simultaneous Determination of the Mycotoxins Deoxynivalenol (DON) and Zearalenone (ZEA) in Soil Matrix

Analysis of Mycotoxins in Beer Using a Portable Nanostructured Imaging Surface Plasmon Resonance Biosensor

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