Denver Papillae Protocol for Objective Analysis of Fungiform Papillae

Nuessle, Tiffany M.; Garneau, Nicole L.; Sloan, Meghan M.; Santorico, Stephanie A.

doi:10.3791/52860-v

Cited by 7 publications

(9 citation statements)

References 13 publications

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“…Evaluation of taste bud density (TBD). Papillae density is calculated using Image J software according to the Denver Papillae Protocol by analazing a reference area (shown as circle) obtained using a standardized procedure 40 …”

Section: Methodsmentioning

confidence: 99%

“…Papillae density is calculated using Image J software according to the Denver Papillae Protocol by analazing a reference area (shown as circle) obtained using a standardized procedure. 40 anaesthesia was applied upon request, using Xylocain Pumpspray dental with 10 mg lidocaine per use. Before papillae removal, a thorough inspection of the oral cavity was performed to assess general tissue health and includes dental status and standardized assessment of periodontitis using a dental probe to perform the periodontal screening.…”

Section: Taste and Smell Perception Including Assessment Of Current H...mentioning

confidence: 99%

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The Obese Taste Bud study: Objectives and study design

Kersten,

Lorenz,

Nottmeier

et al. 2024

Diabetes Obesity Metabolism

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AimsTaste modifies eating behaviour, impacting body weight and potentially obesity development. The Obese Taste Bud (OTB) Study is a prospective cohort study launched in 2020 at the University of Leipzig Obesity Centre in cooperation with the HI‐MAG Institute. OTB will test the hypothesis that taste cell homeostasis and taste perception are linked to obesity. Here, we provide the study design, data collection process and baseline characteristics.Materials and MethodsParticipants presenting overweight, obesity or normal weight undergo taste and smell tests, anthropometric, and taste bud density (TBD) assessment on Day 1. Information on physical and mental health, eating behaviour, physical activity, and dental hygiene are obtained, while biomaterial (saliva, tongue swap, blood) is collected in the fasted state. Further blood samples are taken during a glucose tolerance test. A stool sample is collected at home prior to Day 2, on which a taste bud biopsy follows dental examination. A subsample undergoes functional magnetic resonance imaging while exposed to eating‐related cognitive tasks. Follow‐up investigations after conventional weight loss interventions and bariatric surgery will be included.ResultsInitial results show that glycated haemoglobin levels and age are negatively associated with TBD, while an unfavourable metabolic profile, current dieting, and vegan diet are related to taste perception. Olfactory function negatively correlates with age and high‐density lipoprotein cholesterol.ConclusionInitial findings suggest that metabolic alterations are relevant for taste and smell function and TBD. By combining omics data from collected biomaterial with physiological, metabolic and psychological data related to taste perception and eating behaviour, the OTB study aims to strengthen our understanding of taste perception in obesity.

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Section: Methodsmentioning

confidence: 99%

Section: Taste and Smell Perception Including Assessment Of Current H...mentioning

confidence: 99%

The Obese Taste Bud study: Objectives and study design

Kersten,

Lorenz,

Nottmeier

et al. 2024

Diabetes Obesity Metabolism

View full text Add to dashboard Cite

show abstract

“…In brief, the FP was performed by counting the number of these structures inside three 6 mm-diameter circles virtually drawn on the photo of the subject’s anterior part of the tongue, using Adobe Photoshop software (Adobe Systems Incorporated, San Jose, California) 66 . The count inside each circle was repeated twice by two independent examiners (blinded to food allergy status) following the Denver Papillae Protocol 67 . Then, the mean of the two counts was calculated.…”

Section: Methodsmentioning

confidence: 99%

Alteration of taste perception, food neophobia and oral microbiota composition in children with food allergy

D’Auria¹,

Cattaneo²,

Panelli³

et al. 2023

Sci Rep

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Currently, the mechanisms underlying sensory perception and sensory performance in children with food allergies are far from being understood. As well, only recently, single research afforded the oral host-commensal milieu, addressing oral microbial communities in children with peanut allergies. To bridge the current gaps in knowledge both in the sensory and microbial fields, a psychophysiological case–control study was performed in allergic children (n = 29) and a healthy sex-age-matched control group (n = 30). Taste perception, food neophobia, and liking were compared in allergic and non-allergic children. The same subjects were characterized for their oral microbiota composition by addressing saliva to assess whether specific profiles were associated with the loss of oral tolerance in children with food allergies. Our study evidenced an impaired ability to correctly identify taste qualities in the allergic group compared to controls. These results were also consistent with anatomical data related to the fungiform papillae on the tongue, which are lower in number in the allergic group. Furthermore, distinct oral microbial profiles were associated with allergic disease, with significant down-representations of the phylum Firmicutes and of the genera Veillonella spp., Streptococcus spp., Prevotella spp., and Neisseria spp. For the first time, this study emphasizes the link between sensory perception and food allergy, which is a novel and whole-organism view of this pathology. Our data indicated that an impaired taste perception, as regards both functionality and physiologically, was associated with food allergy, which marginally influences the food neophobia attitude. It is also accompanied by compositional shifts in oral microbiota, which is, in turn, another actor of this complex interplay and is deeply interconnected with mucosal immunity. This multidisciplinary research will likely open exciting new approaches to therapeutic interventions.

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“…This measure was added to control for differences in taste that can be associated with differences in taste anatomy. The protocol for this test is based on the Denver Papillae Protocol 26 . Briefly, subjects rinsed the mouth with deionized water, the tongue was dried with paper towel, and 3 mL of blue food dye at 1:36 concentration was applied to the apex of the tongue using a sterile cotton swab.…”

Section: Methodsmentioning

confidence: 99%

“…The protocol for this test is based on the Denver Papillae Protocol. 26 Briefly, subjects rinsed the mouth with deionized water, the tongue was dried with paper towel, and 3 mL of blue food dye at 1:36 concentration was applied to the apex of the tongue using a sterile cotton swab. The subject was asked to swallow to remove excess dye.…”

Section: Fungiform Papillae Densitymentioning

confidence: 99%

TAS2R38 Haplotype Predicts 24-Hour Urinary Sodium Excretion in Patients With Heart Failure and Their Family Caregivers

et al. 2020

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Background: Adherence to a low-sodium diet is essential to self-care of heart failure (HF). Genetic determinants of preference for high-sodium foods may impede adherence but have not been well-studied. Objective: Our purpose was to examine if TAS2R38 haplotype predicted salt taste sensitivity and dietary sodium intake among patients with HF. Method: This pilot study used baseline data from a large interventional randomized control trial to support adherence to a low-sodium diet in patients with HF and their family caregivers. Participants were tested for salt taste sensitivity and provided a 24-hour urinary sodium sample and a blood sample for DNA analysis at baseline. Fungiform papillae were counted. χ 2 Test and 1-way analysis of variance were used to compare haplotype groups. Linear regression was performed to examine predictors of salt taste sensitivity and 24-hour urinary sodium excretion, controlling for age, gender, ethnicity, smoking status, and fungiform papillae density. Results: There were 42 patients with HF and their family caregivers (age, 64.6 ± 13.4 years, 46.5% male, 97.7% white, and 90.7% nonsmoker). Pronine-alanine-valine homozygous haplotype predicted lower urinary sodium excretion (b = −1780.59, t 41 = −2.18, P = .036), but genotype was not a significant predictor of salt taste sensitivity. Conclusions: The results of our study partially supported our hypothesis that PAV homozygous haplotype predicts 24-hour urinary sodium excretion. With our small sample size, more research is needed. Understanding genetic influences on taste can lead to development of educational interventions tailored to patients with HF and their family caregivers to better support dietary adherence.

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Denver Papillae Protocol for Objective Analysis of Fungiform Papillae

Cited by 7 publications

References 13 publications

The Obese Taste Bud study: Objectives and study design

The Obese Taste Bud study: Objectives and study design

Alteration of taste perception, food neophobia and oral microbiota composition in children with food allergy

TAS2R38 Haplotype Predicts 24-Hour Urinary Sodium Excretion in Patients With Heart Failure and Their Family Caregivers

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