Dentine as a bioactive extracellular matrix

Smith, Anthony J.; Scheven, Ben A.; Takahashi, Yusuke; Ferracane, Jack L.; Shelton, Richard M.; Cooper, Paul R.

doi:10.1016/j.archoralbio.2011.07.008

Cited by 224 publications

(212 citation statements)

References 154 publications

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“…It has been well established that mineralized tissues, specifically both bone and dentine, contain a significant reservoir of sequestered bioactive factors 29, 30, 31, 32, 33, 34. Here it is possible that when presented with a “damaged” dentin surface, these factors were at work in recruiting the injected cGFP‐DPPCs to the site of the “injury.” Osteopontin is expressed by cells in a variety of tissues, including bone, dentin and hypertrophic cartilage; it is a key early marker of mineralized tissue matrix repair and has been postulated to play a role in initially attaching migrated DPPCs to a repair site 35, 36.…”

Section: Discussionmentioning

confidence: 99%

A 3D ex vivo mandible slice system for longitudinal culturing of transplanted dental pulp progenitor cells

et al. 2015

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Harnessing mesenchymal stem cells for tissue repair underpins regenerative medicine. However, how the 3D tissue matrix maintains such cells in a quiescent state whilst at the same time primed to respond to tissue damage remains relatively unknown. Developing more physiologically relevant 3D models would allow us to better understand the matrix drivers and influence on cell‐lineage differentiation in situ. In this study, we have developed an ex vivo organotypic rat mandible slice model; a technically defined platform for the culture and characterization of dental pulp progenitor cells expressing GFP driven by the β‐actin promoter (cGFP DPPCs). Using confocal microscopy we have characterized how the native environment influences the progenitor cells transplanted into the dental pulp. Injected cGFP‐DPPCs were highly viable and furthermore differentially proliferated in unique regions of the mandible slice; in the dentine region, cGFP‐DPPCs showed a columnar morphology indicative of expansion and lineage differentiation. Hence, we demonstrated the systematic capacity for establishing a dental pulp cell‐micro‐community, phenotypically modified in the tooth (the “biology”); and at the same time addressed technical challenges enabling the mandible slice to be accessible on platforms for high‐content imaging (the biology in a “multiplex” format). © 2015 The Authors. Published by Wiley Periodicals, Inc.

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Section: Discussionmentioning

confidence: 99%

A 3D ex vivo mandible slice system for longitudinal culturing of transplanted dental pulp progenitor cells

et al. 2015

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“…These molecules may indicate undifferentiated cells to migrate to the lesion site, proliferate, differentiate into odontoblast-like cells to release organic extracellular matrix, and activate mineralization. [4][5][6] Moreover, high pH contributes to the antibacterial action of pulp capping preparations by having a destructive effect on bacterial cell walls and proteins. 1,7 For that reason, the effectiveness of the bioactive action is directly proportional to the ability of hydroxide ions to dissociate.…”

mentioning

confidence: 99%

Evaluation of pH changes produced by dental pulp capping materials: An in vitro study

Łuczaj-Cepowicz¹,

Marczuk-Kolada²,

Pawińska³

et al. 2017

Dent. Med. Probl.

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“…106 Rejeneratif endodontik tedaviler; trombositlerde ve dentinde bulunan büyüme faktörlerinden faydalanmayı amaçlamak-tadır. 75,107 Yapılan çalışmalarda; dentinin rejeneratif tedavilerde önemli rol oynayan birçok biyoaktif molekül içerdiği gösterilmiştir. 107,108 Büyüme faktörlerinin rejeneratif doku mühendisliğinde önemli rol oynadığı bilinmekle birlikte bu konu hala araştırma safhasındadır.…”

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“…75,107 Yapılan çalışmalarda; dentinin rejeneratif tedavilerde önemli rol oynayan birçok biyoaktif molekül içerdiği gösterilmiştir. 107,108 Büyüme faktörlerinin rejeneratif doku mühendisliğinde önemli rol oynadığı bilinmekle birlikte bu konu hala araştırma safhasındadır. Ancak bu faktörlerin birçoğunun yarı ömürlerinin kısa olması rejeneratif tedavilerde etkin kullanılabilmesine engel olmaktadır.…”

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Revascularization and Treatment Methods

Yılmaz¹,

Nur²,

Tanrıver³

et al. 2016

EÜ Dişhek Fak Derg

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ÖZETKök gelişimi tamamlanmamış daimi dişlerin kanal tedavisi kök uçlarının açık olmasından dolayı zor olmaktadır. Yapılan literatür değerlendirmesinde, kök gelişimini tamamlamamış enfekte daimi dişlerin tedavisinde günümüze kadar birçok tedavi yaklaşımı uygulanmasına rağmen, en uygun tedavi prosedürü ile ilgili kesin bir yaklaşım olmadığı görülmektedir. Bu derlemenin amacı revaskülarizasyon tedavisinde günümüze kadar kullanılan tedavi yöntemlerini ve son zamanlarda diş hekimliğinde kullanılmaya başlanan doku mühendisliği ve kök hücre ile ilgili güncel yaklaşımları değerlendirmektir.

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Dentine as a bioactive extracellular matrix

Cited by 224 publications

References 154 publications

A 3D ex vivo mandible slice system for longitudinal culturing of transplanted dental pulp progenitor cells

A 3D ex vivo mandible slice system for longitudinal culturing of transplanted dental pulp progenitor cells

Evaluation of pH changes produced by dental pulp capping materials: An in vitro study

Revascularization and Treatment Methods

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