Dentin sialoprotein: biosynthesis and developmental appearance in rat tooth germs in comparison with amelogenins, osteocalcin and colagen type-I

Bronckers, A.L.J.J.; D’Souza, Rena N.; Butler, William T.; Lyaruu, D.M.; Dijk, Simon van; Gay, Steffen; Wöltgens, J.H.M.

doi:10.1007/bf00302729

Cited by 85 publications

(59 citation statements)

References 34 publications

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“…Snead et al (1984) isolated RNA from first molars of mice of various ages and found greatest hybridization to a radiolabeled amelogenin probe at birth and in 1 day postnatal mice. Results from immunohistochemistry experiments using anti-amelogenin antibodies have suggested that expression begins in pre-ameloblasts in rat molars (Inai et al, 1991;Bronckers et al, 1993). We, however, have not found evidence for expression in any part of the tooth that does not have some mineralized matrix present.…”

Section: Transgenic Mice Specifically Express Pgalactosidase Activitycontrasting

confidence: 72%

Regulation of amelogenin gene expression during tooth development

Chen

Piddington

Decker

et al. 1994

Developmental Dynamics

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The amelogenins are the predominant matrix proteins in developing enamel and are crucial for proper enamel mineralization. Transgenic mice were constructed in order to identify the segment of the amezogenin gene required for specific expression in enamel organ cells. A 3.5 kb fragment of the bovine X-chromosoma1 amelogenin gene that includes a TATA box, the transcription initiation site, and 32 bp of exon 1 was linked to the pgalactosidase gene and injected into fertilized mouse eggs. Newborn transgene positive mice expressed pgalactosidase activity in developing teeth treated with the chromogenic substrate Xgal. Foci of ameloblasts were positive in newborn mice; stain intensity and number of positive ameloblasts increased in 1-day and 2-day postnatal mice. Some of the adjacent stratum intermedium cells also were positive in the later stages. Targeting of the transgene to the enamel organ was specific; the only other cells observed to be positive were macrophages, which have endogenous pgalactosidase activity.

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Section: Transgenic Mice Specifically Express Pgalactosidase Activitycontrasting

confidence: 72%

Regulation of amelogenin gene expression during tooth development

Chen

Piddington

Decker

et al. 1994

Developmental Dynamics

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“…However, differentiated odontoblasts could not be identified by a unique phenotypic marker; a combination of markers such as Dsp, Dpp, Dmp1, and nestin has been applied to identify these cells (18). Dspp and/or its cleaved products Dsp and Dpp are believed to be tooth-specific (11), although they are also expressed in preameloblasts (6,8,10,15,43,44). Recent studies have shown the expression of Dspp in bone (41,42), cementum (3), and some non-mineralized tissues (2,37,38), while its expression in non-dental tissues is lower than that in dentin.…”

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confidence: 99%

Expression patterns of nestin and dentin sialoprotein during dentinogenesis in mice

et al. 2012

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Differentiated odontoblasts could not be identified by one unique phenotypic marker, but the combination of expression of dentin phosphoprotein (Dpp), dentin sialoprotein (Dsp), dentin matrix protein 1 (Dmp1), and nestin may be valuable for the assessment of these cells. However, the findings using these proteins remain controversial. This study aimed to compare two odontoblast differentiation markers: nestin and Dsp in the process of dentinogenesis in mice. We performed immunohistochemistry and/or in situ hybridization technique for nestin and Dsp using 3-week-old incisors as well as postnatal 1-day-to 8-week-old molars. Preodontoblasts began to express nestin and Dsp proteins and Dsp mRNA, which increased in their intensity according to the progress of odontoblast differentiation in both incisors and developing molars. Nestin was consistently expressed in the differentiated odontoblasts even after the completion of dentin matrix deposition. The expression of Dsp mRNA coincided with the odontoblast secretory activity for dentin matrix deposition. In contrast, other pulpal cells, predentin matrix and dentinal tubules also showed a positive reaction for Dsp protein in addition to differentiated odontoblasts. In conclusion, nestin is valuable as a differentiation marker for odontoblasts, whereas Dsp mRNA is a functional marker for their secretory activity.Dentin is a hard connective tissue that forms the bulk of the tooth, and it is a bone-like matrix characterized by multiple closely packed dentinal tubules that traverse its entire thickness and contain the cytoplasmic processes of odontoblasts, which are responsible for the formation and maintenance of the dentin. The cell bodies of the odontoblasts are aligned along the inner aspect of the dentin, beneath a layer of predentin, where they also form the peripheral boundary of the dental pulp (35). The odontoblasts are terminally differentiated ectomesenchymal cells that synthesize several collagenous and non-collagenous proteins (NCPs) (45). The morphologically discernible differentiation of the odontoblast begins with the dental papilla cells adjacent to the inner enamel epithelium (7). The dental papilla cells adjoining the acellular zone rapidly enlarge and elongate to become preodontoblasts first and then odontoblasts as their cytoplasm increases in volume to contain increasing amounts of proteinsynthesizing organelles. The morphology of odontoblasts reflects their functional activity and ranges from an active synthetic phase to a quiescent phase (35). However, the preferred use of terminology regarding the classification of odontoblasts is controversial, because several terms for differentiated odontoblasts have been used by different researchers, i.e., secretory, transitional, and aged odontoblasts (12), young and old odontoblasts (51), or

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“…Another major dentin NCP, dentin sialoprotein (DSP), is a 53-kDa sialic acid-rich glycoprotein that accounts for 5-8% of the dentin extracellular matrix [Butler et al, 1992]. DSP was reported to be synthesized by odontoblasts, some pulp cells and preameloblasts [Butler et al, 1992;Bronckers et al, 1993]. Immunohistochemical studies showed that DSP was present in predentin and dentin but not in enamel, bone, muscle or cartilage [Butler et al, 1992;Bronckers et al, 1993].…”

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confidence: 99%

Dentin-Pulp Complex Responses to Carious Lesions

Lee¹,

Liu²,

Clarkson³

et al. 2006

Caries Res

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To understand the molecular events underlying the dentin-pulp complex responses to carious progression, we systematically analyzed tissue morphology and dentin matrix protein distribution in non-carious teeth and in teeth with enamel and dentin caries. Dentin matrix proteins analyzed included collagen type I, phosphophoryn (PP) and dentin sialoprotein (DSP), all of which play decisive roles in the dentin mineralization process. Human non-carious and carious third molar teeth were freshly collected, demineralized, and processed for hematoxylin and eosin staining. The ABC-peroxidase method was used for immunohistochemical staining of collagen type I, PP and DSP proteins using specific antibodies. In situ hybridization was also performed. In contrast to elongated odontoblasts in non-carious teeth, odontoblasts subjacent to dentin caries were cuboidal and fewer in number. The predentin zone was also dramatically reduced in teeth with dentin caries. The staining intensity for collagen type I, PP and DSP in the dentin-pulp complex increased progressively from non-carious teeth, to teeth with enamel and dentin caries. In situ hybridization studies showed DSP-PP mRNA expression in odontoblasts and dental pulp that was consistent with our immunohistochemical results. These results suggest that carious lesions stimulate the dentin-pulp complex to actively synthesize collagen type I, PP and DSP proteins. This response to carious lesions is likely to provide a basis for reparative and/or reactionary dentin formation.

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Dentin sialoprotein: biosynthesis and developmental appearance in rat tooth germs in comparison with amelogenins, osteocalcin and colagen type-I

Cited by 85 publications

References 34 publications

Regulation of amelogenin gene expression during tooth development

Regulation of amelogenin gene expression during tooth development

Expression patterns of nestin and dentin sialoprotein during dentinogenesis in mice

Dentin-Pulp Complex Responses to Carious Lesions

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