Dental tissue as an imperative marker for human identification in mass disaster

Khare, Anshika; Saxena, Vrinda; Jain, Manish; Tiwari, Vidhatri; Santha, Binu; Sharva, Vijayta

doi:10.4103/ijfo.ijfo_18_17

Cited by 2 publications

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“…DNA concentration was significantly correlated with the duration of exposure. This is similar to the study carried out by Khare et al, (2018). In studies by Vermuri et al (2012), the amount of DNA kept decreasing as the number of days increased.…”

Section: Bajopas Volume 15 Number 1 June 2022supporting

confidence: 88%

“…Compared to DNA purity, time of environmental exposure within groups of environmental conditions, showed no significant difference. This was supported by a study carried out by Khare et al, (2018) that showed that DNA purity was not significantly affected by the storage period of teeth in soil. More premolars and molars were analyzed compared to the number of canine and incisors for this study.…”

Section: Bajopas Volume 15 Number 1 June 2022mentioning

confidence: 70%

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Effect of different environmental conditions on quality of DNA extracted from human teeth for gender determination

Iroanya¹,

Onuoha²

2022

Bayero J. Pure App. Sci.

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Use of morphological characteristics in human identification maybe difficult in conditions where human remains are grossly fragmented, decomposed and/or mutilated, or when only a tooth is available. The study aims to ascertain and compare the quantity of DNA from teeth exposed to different environmental conditions using organic and silica column DNA extraction methods and determine their gender using amelogenin gene primer. Human teeth were used and divided into four major groups based on different exposure types (buried in soil, storage in Lagoon water, normal environmental condition by placing them on the floor at the botanical garden, and exposure to temperature of 210oC for 15mins). The powdered dentin-cementum complex was used for DNA extraction, quantification and purity assessments. Amplification was done using amelogenin gene primers. Mean DNA concentration and purity were 187.59±26.25ng/μl and 0.97±0.07 respectively for silica column method, and, 138.98±35.71ng/μl and 0.91±0.11 respectively for organic extraction. With organic extraction, the least and highest mean DNA concentration are from teeth immersed in Lagoon water (74.86±61.19ng/μl) and teeth exposed to temperature of 210oC for 15mins (385.14±106.39ng/μl). In the Silica column method, teeth heated at 210oC for 15mins showed the lowest mean DNA concentration (165.57±33.82ng/μl) while teeth exposed to "normal environmental conditions", gave the highest DNA concentration (218.46±45.03ng/μl). Positive and negative male identification were 30.8% and 69.2% respectively, while female identification was 100% positive irrespective of the environmental exposure. The dentine-cementum is a valuable DNA source for forensic investigations. Male identification using amelogenin gene, should still be investigated in the Nigerian context.

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Section: Bajopas Volume 15 Number 1 June 2022supporting

confidence: 88%

Section: Bajopas Volume 15 Number 1 June 2022mentioning

confidence: 70%

Effect of different environmental conditions on quality of DNA extracted from human teeth for gender determination

Iroanya¹,

Onuoha²

2022

Bayero J. Pure App. Sci.

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“…Dentin and pulp DNA is more affected than cemental DNA by the disease process 10,11 . Pulp is the most preferred source for DNA as it yielded a good quantity and highest amplification of DNA followed by dentin and cementum which were identical 20,64 . Further pulp volume decreases with age rendering lesser tissue for DNA analysis in older individuals compared to young individuals.…”

Section: Remains In An Advanced State Of Decompositionmentioning

confidence: 99%

Tooth as a Source of DNA in Forensic or Investigative Genetics: An Overview

Nandini

Joji²

2022

jfds

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Deoxyribonucleic acid (DNA) is the genetic code of most organisms including humans. In the last few years, DNA analysis methods are applied to forensic cases for human identification which is termed forensic or investigative genetics. It is often challenging to obtain and interpret DNA from routine samples like blood in severely decomposed or disfigured bodies recovered from incineration, immersion, trauma, mutilation, and decomposition as in incidents of fire, explosion, or murder. The tooth can survive any extreme environment with minimum risk of contamination making it a valuable alternative source of DNA in such cases. Tooth structures like the enamel (amelogenin protein), dentin, cementum, pulp, and adherent tissues like bone and periodontal fibers are sources from which DNA can be obtained. Various methods have been described for DNA extraction. There are numerous applications of DNA analysis like identification of the deceased or missing victim or the unknown culprit from a crime scene, solving paternity issues, determining the occurrence of any genetic disease, and determining the ancestry. This article briefly summarizes an overview of the tooth as a valuable DNA source and various methods and challenges related to DNA analysis.

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Dental tissue as an imperative marker for human identification in mass disaster

Cited by 2 publications

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Effect of different environmental conditions on quality of DNA extracted from human teeth for gender determination

Effect of different environmental conditions on quality of DNA extracted from human teeth for gender determination

Tooth as a Source of DNA in Forensic or Investigative Genetics: An Overview

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