Dental Stem Cell in Tooth Development and Advances of Adult Dental Stem Cell in Regenerative Therapies

Tan, Jiali; Xu, Xin; Lin, Jiong; Li, Fan; Zheng, Yuting; Wei, Kaihua

doi:10.2174/1574888x09666141002122637

Cited by 4 publications

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“…However, the role of MSCs transplantation in regeneration of hepatocytes remains controversial [26]. The majority of recent studies indicated that the trophic and immunomodulatory factors secreted by MSCs play a crucial role in liver injury [4, 5, 12, 27]. In the current study, we demonstrated that MSCs increased the mRNA and protein levels of HO-1 in liver, which led to significant improvement in liver injury, inflammatory response, and neutrophil infiltration.…”

Section: Discussionsupporting

confidence: 57%

“…No artificial liver device has emerged as a way to bridge patients with acute liver disease to transplantation or recovery. Recently, some stem cell therapies have shown promise in treating acute liver failure and drug-induced liver injury [4–6]. …”

Section: Introductionmentioning

confidence: 99%

“…Several studies have demonstrated the therapeutic effect of MSCs in ALF [4–6]. However, the relationship between HO-1, neutrophils, and the therapeutic effect of MSCs in ALF has not been demonstrated.…”

Section: Introductionmentioning

confidence: 99%

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Mesenchymal stem cells increase expression of heme oxygenase-1 leading to anti-inflammatory activity in treatment of acute liver failure

et al. 2017

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BackgroundMesenchymal stem cells (MSCs) have been studied for the treatment of acute liver failure (ALF) for several years. MSCs may exert their effect via complex paracrine mechanisms. Heme oxygenase (HO) 1, a rate-limiting enzyme in heme metabolism, exerts a wide range of anti-inflammatory, anti-apoptotic and immunoregulatory effects in a variety of diseases. However, the relationship between MSCs and HO-1 in the treatment of ALF is still unclear. We investigated the preventive and therapeutic potential of intravenously administered BMSCs.MethodsBone marrow-derived mesenchymal stem cells (BMSCs) obtained from Sprague–Dawley rats were isolated and cultured. We employed BMSCs, hemin (a HO-1 inducer) and zinc protoporphyrin (ZnPP, the HO-1 activity inhibitor) in D-galactosamine (D-Gal)/lipopolysaccharides (LPS)-induced ALF rats. Rats were sacrificed at days 1, 3, 5, and 7 post-transfusion, respectively. Blood samples and liver tissues were collected. Hepatic injury, HO-1 activity, chemokines, inflammatory cytokines, the number and oxidative activity of neutrophils, ki67, and TUNEL-positive cells were evaluated.ResultsHO-1 induction or BMSCs transplantation attenuated D-galactosamine/lipopolysaccharide-induced increases in alanine aminotransferase, aspartate aminotransferase, total bilirubin (TBIL), ammonia, and inflammatory cytokines. Treatment with hemin or BMSCs also inhibited neutrophil infiltration, oxidative activity, and hepatocyte apoptosis. The protective effect of BMSCs was partially neutralized by ZnPP, suggesting the key role of HO-1 in the process.ConclusionsThese findings may correlate with inhibition of nuclear factor-κ B activation. BMSCs ameliorated ALF by increasing the HO-1 expression, which reduced PMN infiltration and function, and played an important anti-inflammatory and anti-apoptotic role.Graphical AbstractProposed mechanism by which BMSCs reduce inflammation, neutrophil activation, and hepatocyte apoptosis and promote hepatocyte proliferation via HO-1. BMSCs increase HO-1 expression in liver via Nrf2. HO-1 protects against LPS/D-Gal-induced ALF by inhibiting neutrophil infiltration and inflammatory burst, and hepatocyte apoptosis and necrosis. HO-1 also promotes hepatocyte proliferation. Electronic supplementary materialThe online version of this article (doi:10.1186/s13287-017-0524-3) contains supplementary material, which is available to authorized users.

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Section: Discussionsupporting

confidence: 57%

Section: Introductionmentioning

confidence: 99%

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Mesenchymal stem cells increase expression of heme oxygenase-1 leading to anti-inflammatory activity in treatment of acute liver failure

et al. 2017

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“…PLSCs have the ability to multidifferentiate in vitro into collagen-forming cells, cementoblast-like cells, osteoblasts, chondrocytes, adipocytes as well (Tan et al, 2015). Above all, PLSCs possibly will produce cementum/ PDL tissue in vivo, that affords an optimum cell source for regeneration of periodontal tissue (Iwata et al, 2009;Flores et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

Influence of Different Concentrations of Human Platelet Rich Plasma Versus Fetal Bovine Serum on Periodontal Ligament Derived Stem Cells

Abdelfattah

Din²

2022

Middle East Journal of Medical Genetics

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Periodontitis is a complex immune-inflammatory disease, characterized by the destruction of the periodontium. Periodontal regeneration implicates the use of mesenchymal stem cell populations for cell-based therapies. Periodontal ligament stem cells (PLSCs) isolated from the periodontal (PDL) tissue of human teeth, are the most extensively studied and applied for regeneration of the periodontal tissues. Platelet rich plasma (PRP) is widely used for various tissue engineering applications, because of its several advantages over fetal bovine serum (FBS). In this study, we isolated PLSCs from four extracted sound wisdom teeth. Venous blood was drawn from healthy male and activated PRP was prepared. The effect of various concentrations of human PRP (hPRP) in comparison with 10% FBS on PLSCs proliferation and osteogenic differentiation have been detected. Statistical analysis was done using a One-way analysis of variance (ANOVA). Our study showed that hPRP can effectively induce hPLSC's proliferation as well as stimulate osteogenic differentiation of hPLSC's. The 10% hPRP had the highest proliferation rate than 5% hPRP, 15% hPRP as well as 10% FBS with statistical significance at 3 and 5 days' groups. The RT-PCR showed that osteopontin gene in 10% FBS expressed higher than all PRP groups with no statistical significance and the osteopontin gene expression in the 10% hPRP group was higher than the 5% PRP and 15% PRP with no statistical significance.

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“…The ideal stem cell for stem cell-based therapy still remains to be elucidated. In the past, although various types of stem cells have been isolated from teeth (dental pulp stem cells (P-MSCs), periodontal ligament stem cells (PDL-MSCs), stem cells from exfoliated human deciduous teeth (SHED), stem cells from root apical papilla (SCAP), dental follicle progenitor stem cells, Gingival Mesenchymal stem cells (GMSCs) [26][27][28][29], orofacial bone/bone-marrow-derived MSCs [26][27][28][29], Tooth germ stem cells (TGSCs) [26][27][28][29], MSCs from periapical lesions (PL-MSCs) [26][27][28][29], Oral mucosal lamina propriaderived progenitor cells (OMLP-PCs) Ding et al [30] and granulation tissue from tooth extraction socket [31] but the ideal stem cell is still questionable, including, which may be a good stem cell type for stem cell-based therapy in certain disease, especially when they origin from neural crest is considered [32]. Hakki et al [32] and Lei et al [33] conducted studies to compare P-MSCs and PDL-MSCs and found higher proliferation potential and telomerase activity in P-MSCs.…”

Section: Discussionmentioning

confidence: 99%

Periodontal Ligament Stem Cells-The Regeneration Front

Gothi¹

2015

Dentistry

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The principal cells of the healthy, functional periodontal ligament are the differentiated cells and their progenitors. The differentiated cells are concerned with the synthesis and resorption of alveolar bone and the fibrous connective tissue of the ligament and cementum. It has been shown that small proportion of periodontal ligament cells are in mitosis at any given time. The cells of the periodontal ligament are also highly active metabolically; those of the rat molar turn the collagen over 5 times faster than do the fibroblast in the lamina propria of gingiva and 15 times faster than the fibroblast of skin. Progenitor cells exhibited marked differences in cytodifferentiation, suggesting that the progenitor cells that divide after wounding do not belong to a population of cells exhibiting uniform cytological characteristics, therefore it is unlikely that ultrastructural cytology can be used to identify these progenitor cells. Progenitor cells exhibited marked differences in cytodifferentiation, suggesting that the progenitor cells that divide after wounding do not belong to a population of cells exhibiting uniform cytological characteristics, therefore it is unlikely that ultrastructural cytology can be used to identify these progenitor cells. The evidence that these cells are present within the periodontal tissues was provided by McCulloch and coworkers by in vivo and histological studies.

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Dental Stem Cell in Tooth Development and Advances of Adult Dental Stem Cell in Regenerative Therapies

Cited by 4 publications

References 0 publications

Mesenchymal stem cells increase expression of heme oxygenase-1 leading to anti-inflammatory activity in treatment of acute liver failure

Mesenchymal stem cells increase expression of heme oxygenase-1 leading to anti-inflammatory activity in treatment of acute liver failure

Influence of Different Concentrations of Human Platelet Rich Plasma Versus Fetal Bovine Serum on Periodontal Ligament Derived Stem Cells

Periodontal Ligament Stem Cells-The Regeneration Front

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