Dental Pulp Stem Cells Transplantation Improves Passive Avoidance Memory and Neuroinflammation in Trimethyltin-Induced Alzheimer’s Disease Rat Model

Malekzadeh, Samira; Edalatmanesh, Mohammad Amin; Mehrabani, Davood; Shariati, Mehrdad

doi:10.31661/gmj.v10i0.2254

Cited by 5 publications

(5 citation statements)

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“…An intraperitoneal injection of scopolamine was administered to cause cholinergic malfunction and impair cognition in rats. Scopolamine has been shown to decrease ChAT activity, which plays an important role in Ach synthesis in the cortex of patients with AD [ 68 ]. Amnesia induced by scopolamine has been suggested as a model for studying dementia in cases where the cholinergic function is a doubted probable cause [ 69 ]; scopolamine (1 mg/kg, IP) was found to decrease the behaviour of animals in the Y-maze test [ 70 ].…”

Section: Methodsmentioning

confidence: 99%

Extract of Aster koraiensis Nakai Leaf Ameliorates Memory Dysfunction via Anti-inflammatory Action

Lee¹,

Park²,

Jang³

et al. 2023

IJMS

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Aster koraiensis Nakai (AK) leaf reportedly ameliorates health problems, such as diabetes. However, the effects of AK on cognitive dysfunction or memory impairment remain unclear. This study investigated whether AK leaf extract could attenuate cognitive impairment. We found that AK extract reduced the production of nitric oxide (NO), tumour necrosis factor (TNF)-α, phosphorylated-tau (p-tau), and the expression of inflammatory proteins in lipopolysaccharide- or amyloid-β-treated cells. AK extract exhibited inhibitory activity of control specific binding on N-methyl-D-aspartate (NMDA) receptors. Scopolamine-induced AD models were used chronically in rats and acutely in mice. Relative to negative controls (NC), hippocampal choline acetyltransferase (ChAT) and B-cell lymphoma 2 (Bcl2) activity was increased in rats chronically treated with scopolamine and fed an AK extract-containing diet. In the Y-maze test, spontaneous alterations were increased in the AK extract-fed groups compared to NC. Rats administered AK extract showed increased escape latency in the passive avoidance test. In the hippocampus of rats fed a high-AK extract diet (AKH), the expression of neuroactive ligand–receptor interaction-related genes, including Npy2r, Htr2c, and Rxfp1, was significantly altered. In the Morris water maze assay of mice acutely treated with scopolamine, the swimming times in the target quadrant of AK extract-treated groups increased significantly to the levels of the Donepezil and normal groups. We used Tg6799 Aβ-overexpressing 5XFAD transgenic mice to investigate Aβ accumulation in animals. In the AD model using 5XFAD, the administration of AK extract decreased amyloid-β (Aβ) accumulation and increased the number of NeuN antibody-reactive cells in the subiculum relative to the control group. In conclusion, AK extract ameliorated memory dysfunction by modulating ChAT activity and Bcl2-related anti-apoptotic pathways, affecting the expression of neuroactive ligand–receptor interaction-related genes and inhibiting Aβ accumulation. Therefore, AK extract could be a functional material improving cognition and memory.

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Section: Methodsmentioning

confidence: 99%

Extract of Aster koraiensis Nakai Leaf Ameliorates Memory Dysfunction via Anti-inflammatory Action

Lee¹,

Park²,

Jang³

et al. 2023

IJMS

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“…The first-strand cDNA was prepared applying the Revert Aid™ first strand cDNA synthesis kit (Thermo Fisher, Waltham, MA, USA). PCR thermal cycler (Veriti Thermal Cycler, Foster City, CA, USA) was used for PCR runs, including 1 cycle at 94 °C for 3 min, 35 cycles at 94 °C for 30 s, 60 °C for 30 s, and 72 °C for 30 s, and 1 cycle at 72 °C for 10 min, and, finally, the bands were visualized by electrophoresis [ 30 ] ( Table 1 ).…”

Section: Methodsmentioning

confidence: 99%

Cell Proliferation, Viability, Differentiation, and Apoptosis of Iron Oxide Labeled Stem Cells Transfected with Lipofectamine Assessed by MRI

Jalli

Mehrabani

Zare

et al. 2023

JCM

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To assess in vitro and in vivo tracking of iron oxide labeled stem cells transfected by lipofectamine using magnetic resonance imaging (MRI), rat dental pulp stem cells (DPSCs) were characterized, labeled with iron oxide nanoparticles, and then transfected with lipofectamine to facilitate the internalization of these nanoparticles. Cell proliferation, viability, differentiation, and apoptosis were investigated. Prussian blue staining and MRI were used to trace transfected labeled cells. DPSCs were a morphologically spindle shape, adherent to culture plates, and positive for adipogenic and osteogenic inductions. They expressed CD73 and CD90 markers and lacked CD34 and CD45. Iron oxide labeling and transfection with lipofectamine in DPSCs had no toxic impact on viability, proliferation, and differentiation, and did not induce any apoptosis. In vitro and in vivo internalization of iron oxide nanoparticles within DPSCs were confirmed by Prussian blue staining and MRI tracking. Prussian blue staining and MRI tracking in the absence of any toxic effects on cell viability, proliferation, differentiation, and apoptosis were safe and accurate to track DPSCs labeled with iron oxide and transfected with lipofectamine. MRI can be a useful imaging modality when treatment outcome is targeted.

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“…Researchers have demonstrated scopolamineinduced memory failure due to oxidative brain damage. Scopolamine-induced modeling is therefore used to screen for anti-dementia drugs in experimental animals 3 . The memory loss caused by scopolamine is commonly used due to the noninvolvement of any surgical procedures 9 .…”

Section: Animal Modelsmentioning

confidence: 99%

“…Alzheimer's disease: Alzheimer's dementia is a degenerative brain disorder that could be categorized by behavioral deterioration and memory loss, concentration deficiency, and a gradual reduction in linguistic efficiency. Many factors can trigger AD, including congenital abnormalities, neurofibrillary tangle development, impaired processing of amyloid progenitors, mitochondrial deficiencies, neurotrophic factor deficiency, neurotoxicity of trace elements, defective of energy metabolism, as well as oxidative damage 3 . A depletion of short-term memory, failure to learn new knowledge, mood changes, difficulty recognizing terms, forgetting identities and losing things are demonstrated by earlier illness.…”

Section: Introductionmentioning

confidence: 99%

Untitled

2022

IJPSR

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Characteristics like dementia, behavioural and cognitive dysfunction, and memory decline may characterize Alzheimer's disease (AD). A deterioration of short-term memory, inability to acknowledge new information, mood swings, difficulty recognizing words, forgetting names, and losing things are demonstrated by early disease. Popular emotional characteristics displayed by patients with AD are anger, aggression, and irritability. Today's world attempts to treat various ailments have increased with medical advancements. There is still considerable research into medications capable of preventing or at least effectively altering the trajectory of AD, referred to as 'disease-modifying medicines. Laboratory animals are helping to test new ways of treating illness. For the goal of developing therapeutics or disease-modifying agents, a vast array of experimental models were developed to simulate the human context of the illness. In animal studies, AD caused by chemical compounds may be helpful in deeper understanding the mechanism of disease and AD treatment. The anticholinergic drug Scopolamine induces amnesia in rodents. This analysis examines the various animal models frequently used to research the effect of novel drugs or medicinal herbs on chemical-induced Alzheimer's disease, such as scopolamine. This article provides an explanation of behavioral testing and the assessment of brain enzyme levels.

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Dental Pulp Stem Cells Transplantation Improves Passive Avoidance Memory and Neuroinflammation in Trimethyltin-Induced Alzheimer’s Disease Rat Model

Cited by 5 publications

References 0 publications

Extract of Aster koraiensis Nakai Leaf Ameliorates Memory Dysfunction via Anti-inflammatory Action

Extract of Aster koraiensis Nakai Leaf Ameliorates Memory Dysfunction via Anti-inflammatory Action

Cell Proliferation, Viability, Differentiation, and Apoptosis of Iron Oxide Labeled Stem Cells Transfected with Lipofectamine Assessed by MRI

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