Dental pulp stem cells senescence and regenerative potential relationship

Iezzi, Iolanda; Cerqueni, Giorgia; Licini, Caterina; Lucarini, Guendalina; Mattioli‐Belmonte, Monica

doi:10.1002/jcp.27472

Cited by 40 publications

(31 citation statements)

References 37 publications

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“…With increasing age, endogenous lysosomal galactosidase (b-Gal) is overexpressed, leading to its accumulation in senescent cells; therefore, b-Gal expression is often used to detect cellular senescence (Kurz et al 2000). As expected, SA-b-Gal expression was stronger in ADPSCs than in JDPSCs, in line with other reports (Feng et al 2014, Iezzi et al 2019.…”

Section: Discussionsupporting

confidence: 93%

“…Ageing affects inflammatory DPSCs Ning et al with previous reports (Feng et al 2014, Iezzi et al 2019. With increasing age, endogenous lysosomal galactosidase (b-Gal) is overexpressed, leading to its accumulation in senescent cells; therefore, b-Gal expression is often used to detect cellular senescence (Kurz et al 2000).…”

Section: Discussionmentioning

confidence: 99%

“…The proliferation, osteo/odontogenic differentiation and neurogenic differentiation potentials of DPSCs decline with age (Iezzi et al . ). Previous studies indicated that DPSCs isolated from juvenile rats had greater proliferation and lower osteogenic differentiation abilities than DPSCs from adult rats (Ma et al .…”

Section: Introductionmentioning

confidence: 97%

“…Similar to other tissues, dental pulp tissues also undergo age-related changes, including alteration of the biological characteristics of DPSCs. The proliferation, osteo/odontogenic differentiation and neurogenic differentiation potentials of DPSCs decline with age (Iezzi et al 2019). Previous studies indicated that DPSCs isolated from juvenile rats had greater proliferation and lower osteogenic differentiation abilities than DPSCs from adult rats (Ma et al 2009).…”

Section: Introductionmentioning

confidence: 99%

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Ageing affects the proliferation and mineralization of rat dental pulp stem cells under inflammatory conditions

et al. 2019

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Aim To comparatively evaluate changes in the proliferation and mineralization abilities of dental pulp stem cells (DPSCs) from juvenile and adult rats in a lipopolysaccharide (LPS)‐induced inflammatory microenvironment to provide a theoretical basis for the age‐related differences observed in DPSCs during repair of inflammatory injuries. Methodology DPSCs were isolated from juvenile (JDPSCs) and adult rats (ADPSCs), and senescence‐associated β‐galactosidase staining was used to compare senescence between JDPSCs and ADPSCs. Effects of LPS on JDPSCs and ADPSCs proliferation were investigated by cell counting kit‐8 assays and flow cytometry. Alizarin red staining, quantitative reverse transcription polymerase chain reaction and Western blot assay were used to examine the effects of LPS on mineralization‐related genes and proteins in JDPSCs and ADPSCs. Immunohistochemistry was used to compare interleukin‐1β (IL‐1β) and osteocalcin (OCN) expression in the pulpitis model. Unpaired Student's t‐tests and one‐way anova were used for statistical analysis. Results DPSCs were isolated from juvenile and adult rat dental pulp tissues. At low concentrations (0.1–1 μg mL−1), LPS significantly promoted the proliferation of JDPSCs (P < 0.01) and ADPSCs (P < 0.01 or P < 0.05), with the effect being stronger in JDPSCs than in ADPSCs. In addition, mineralized nodules and the expression of mineralization‐related genes (OCN, DSPP, ALP, BSP) increased significantly after stimulation with LPS (0.5 μg mL−1) in JDPSCs and ADPSCs (P < 0.01 or P < 0.05), and JDPSCs displayed a more obvious increase than ADPSCs. Western blots revealed OCN and ALP expression levels in JDPSCs treated with LPS were significantly upregulated (P < 0.05); meanwhile, ALP expression in ADPSCs increased slightly but significantly (P < 0.05), and OCN expression was not affected. Finally, IL‐1β expression was significantly higher (P < 0.05) and OCN expression was significantly lower (P < 0.05) in the inflamed dental pulp of adult rats than in juvenile rats. Conclusions A certain degree of inflammatory stimulation promoted the proliferation and mineralization of DPSCs; however, this effect declined with age. The DPSCs of adult donors in an inflammatory microenvironment have a weaker repair ability than that of juvenile donors, who are better candidates for tissues damage repair.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

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Ageing affects the proliferation and mineralization of rat dental pulp stem cells under inflammatory conditions

et al. 2019

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“…Ageing also affects the ability of DPSCs to contribute to mineralisation processes. In fact, a decreased osteogenic potential was observed in aged human DPSCs (Yi et al, 2017;Iezzi et al, 2019). The decline of differentiation potential towards mineralised tissues (i.e.…”

Section: Impact Of Ageing On Dental Pulp Stem Cellsmentioning

confidence: 96%

The effects of ageing on dental pulp stem cells, the tooth longevity elixir

Iezzi¹,

Pagella²,

Mattioli‐Belmonte³

et al. 2019

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Stem cells are essential for tissue homeostasis and regeneration throughout the lifespan of multicellular organisms. The decline in stem cell function during advanced age is associated with a reduced regenerative potential of tissues that leads to an increased frequency of diseases. Age-related changes also occur in the dental pulp that represents a reliable model tissue, with high regenerative capability, for studying senescence mechanisms. However, little information is available concerning the effects of ageing on dental stem-cell function. In this mini-review, recent data on how the molecular and functional alterations that accumulate in stem cell populations during ageing result in modifications of dental pulp physiology are discussed. Changes that accumulate during ageing such as how reduction of pulp chamber volume, decreased vascular supply and modifications to the stem cell niches affect stem cell functions and, therefore, dental pulp regenerative potential in response to various stressful agents. Dental pulp cells from aged individuals are still metabolically active and secrete pro-inflammatory and matrix-degrading molecules. Furthermore, miRNAs and exosomes derived from dental pulp stem cells constitute an attractive source of nanovesicles for the treatment of age-related dental pathologies. Further investigation of the epigenetic alterations in dental pulp stem cells, accumulating during ageing, might reveal crucial information for potential stem cell-based therapeutic approaches in the elderly.

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