Dental pulp stem cells in chitosan/gelatin scaffolds for enhanced orofacial bone regeneration

Bakopoulou, Athina; Georgopoulou, Αnthie; Grivas, Ioannis; Bekiari, Chryssa; Prymak, Oleg; Loza, Κateryna; Epple, Matthias; Papadopoulos, George C.; Koidis, Petros; Chatzinikolaidou, Μaria

doi:10.1016/j.dental.2018.11.025

Cited by 66 publications

(55 citation statements)

References 65 publications

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“…Examples include incorporating chitosan into fibrin hydrogel for pulp regeneration, glass ionomer cement, scaffold, guided tissue regeneration (GTR) membrane, and in tissue conditioner for treating denture stomatitis. [20][21][22][23][24] The purpose of the present research was to develop chitosanbased denture adhesive that inhibits the adherence and growth of Candida species on denture base acrylic resin. Among the chitosan derivatives with different chemical characteristics that were tested against common oral Candida species, HMWC has the most effective fungicidal activity against both C albicans and many common NAC species.…”

Section: Discussionmentioning

confidence: 99%

“…14,15 Several applications in dentistry have been reported, including in endodontics, periodontics, and tissue engineering and as a drug delivery system. [18][19][20][21][22][23][24] Chitosan has broad-spectrum antimicrobial activity against bacteria and fungi by disrupting cell membranes and leading to intracellular leakage of ions and may inhibit deoxyribonucleic acid (DNA), ribonucleic acid (RNA), and protein synthesis. 16,25 Many chitosan derivatives exist, and their properties and antimicrobial activity vary with the molecular weight, degree of deacetylation, pH, and other factors.…”

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confidence: 99%

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Activity of chitosan antifungal denture adhesive against common Candida species and Candida albicans adherence on denture base acrylic resin

Namangkalakul

Benjavongkulchai²,

Pochana

et al. 2020

The Journal of Prosthetic Dentistry

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Activity of chitosan antifungal denture adhesive against common Candida species and Candida albicans adherence on denture base acrylic resin

Namangkalakul

Benjavongkulchai²,

Pochana

et al. 2020

The Journal of Prosthetic Dentistry

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“…In vitro tests with DPSC showed no cytotoxic effects and scaffold mineralization over 8 weeks. In vivo experiments in mice confirmed high amounts of osteoid and fully mineralized bone [86].…”

Section: Polysaccharide Scaffoldsmentioning

confidence: 79%

Polysaccharide-Based Systems for Targeted Stem Cell Differentiation and Bone Regeneration

et al. 2019

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Bone tissue engineering is an ever-changing, rapidly evolving, and highly interdisciplinary field of study, where scientists try to mimic natural bone structure as closely as possible in order to facilitate bone healing. New insights from cell biology, specifically from mesenchymal stem cell differentiation and signaling, lead to new approaches in bone regeneration. Novel scaffold and drug release materials based on polysaccharides gain increasing attention due to their wide availability and good biocompatibility to be used as hydrogels and/or hybrid components for drug release and tissue engineering. This article reviews the current state of the art, recent developments, and future perspectives in polysaccharide-based systems used for bone regeneration.

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“…The results of individual studies are presented in Tables 2-6. In order to evaluate the efficacy of chitosan-based scaffolds during bone regeneration, data about cell proliferation and viability were recorded from 10 of the included articles: five of them used the MTT assay of cementoblasts [18][19][20], periodontal ligament cells [18,28,37], one used the Cell-Counting Kit test (CCK-8) [23,26], four applied the AlamarBlue assay [25,29,30,32] and one the PrestoBlue test [22] (Tables 2-4). ALP activity of periodontal ligament cells [21], osteoblasts [34,35], and mesenchymal stem or stromal cells [24] was assessed in four studies (Table 5).…”

Section: Results Of Individual Studiesmentioning

confidence: 99%

Nanomaterials for Periodontal Tissue Engineering: Chitosan-Based Scaffolds. A Systematic Review

et al. 2020

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Introduction. Several biomaterials are used in periodontal tissue engineering in order to obtain a three-dimensional scaffold, which could enhance the oral bone regeneration. These novel biomaterials, when placed in the affected area, activate a cascade of events, inducing regenerative cellular responses, and replacing the missing tissue. Natural and synthetic polymers can be used alone or in combination with other biomaterials, growth factors, and stem cells. Natural-based polymer chitosan is widely used in periodontal tissue engineering. It presents biodegradability, biocompatibility, and biological renewability properties. It is bacteriostatic and nontoxic and has hemostatic and mucoadhesive capacity. The aim of this systematic review is to obtain an updated overview of the utilization and effectiveness of chitosan-based scaffold (CS-bs) in the alveolar bone regeneration process. Materials and Methods. During database searching (using PubMed, Cochrane Library, and CINAHL), 72 items were found. The title, abstract, and full text of each study were carefully analyzed and only 22 articles were selected. Thirteen articles were excluded based on their title, five after reading the abstract, twenty-six after reading the full text, and six were not considered because of their publication date (prior to 2010). Quality assessment and data extraction were performed in the twelve included randomized controlled trials. Data concerning cell proliferation and viability (CPV), mineralization level (M), and alkaline phosphatase activity (ALPA) were recorded from each article Results. All the included trials tested CS-bs that were combined with other biomaterials (such as hydroxyapatite, alginate, polylactic-co-glycolic acid, polycaprolactone), growth factors (basic fibroblast growth factor, bone morphogenetic protein) and/or stem cells (periodontal ligament stem cells, human jaw bone marrow-derived mesenchymal stem cells). Values about the proliferation of cementoblasts (CB) and periodontal ligament cells (PDLCs), the activity of alkaline phosphatase, and the mineralization level determined by pure chitosan scaffolds resulted in lower than those caused by chitosan-based scaffolds combined with other molecules and biomaterials. Conclusions. A higher periodontal regenerative potential was recorded in the case of CS-based scaffolds combined with other polymeric biomaterials and bioceramics (bio compared to those provided by CS alone. Furthermore, literature demonstrated that the addition of growth factors and stem cells to CS-based scaffolds might improve the biological properties of chitosan.

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Dental pulp stem cells in chitosan/gelatin scaffolds for enhanced orofacial bone regeneration

Cited by 66 publications

References 65 publications

Activity of chitosan antifungal denture adhesive against common Candida species and Candida albicans adherence on denture base acrylic resin

Activity of chitosan antifungal denture adhesive against common Candida species and Candida albicans adherence on denture base acrylic resin

Polysaccharide-Based Systems for Targeted Stem Cell Differentiation and Bone Regeneration

Nanomaterials for Periodontal Tissue Engineering: Chitosan-Based Scaffolds. A Systematic Review

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