Dental pulp stem cells for the study of neurogenetic disorders

Victor, A. Kaitlyn; Reiter, Lawrence T.

doi:10.1093/hmg/ddx208

Cited by 40 publications

(40 citation statements)

References 53 publications

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“…Inhibition of glutamate release has also been used previously as a validated cell assay for measuring the potential activity of Arg0 on the inhibition of neuronal exocytosis 29 . Primary human dental pulp stem cells derived neurons (DPSC neurons) were chosen due to its ability to differentiate into functionally active neurons and reduced problems associated with epigenetic changes and re-programming, as compared to induced pluripotent stem cells 30 . Comparing to the undifferentiated dental pulp stem cells (DPSC), a significantly higher fluorometric readout (indicative of the amount of glutamate released) was observed in the neuronally differentiated DPSC neuron group, as compared to blank and DPSC group (Fig.…”

Section: Resultsmentioning

confidence: 99%

Enhanced Skin Permeation of Anti-wrinkle Peptides via Molecular Modification

Lim

Sun

Madanagopal

et al. 2018

Sci Rep

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Wrinkles can have a negative effect on quality of life and Botox is one of the most effective and common treatments. Argireline (Arg0), a mimetic of Botox, has been found to be safer than Botox and effective in reducing wrinkles, with efficacies up to 48% upon 4 weeks of twice daily treatment. However, the skin permeation of Arg0 is poor, due to its large molecular weight and hydrophilicity. Arg0 exists in zwitterionic form and this charged state hindered its skin permeation. Chemical modification of the peptide structure to reduce the formation of zwitterions may result in increased skin permeability. We investigated a total of 4 peptide analogues (Arg0, Arg1, Arg2, Arg3), in terms of skin permeation and wrinkle reduction. The 4 peptides were dissolved in various propylene glycol and water co-solvents. Enhanced human skin permeation was demonstrated by both Arg2 and Arg3 in vitro. On the other hand, the abilities of the 4 analogues to reduce wrinkle formation were also compared using primary human dental pulp stem cells derived neurons. By measuring the inhibition of glutamate release from the neurons in vitro, it was shown that Arg3 was the most effective, followed by Arg1, Arg0 and Arg2.

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Section: Resultsmentioning

confidence: 99%

Enhanced Skin Permeation of Anti-wrinkle Peptides via Molecular Modification

Lim

Sun

Madanagopal

et al. 2018

Sci Rep

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“…In parallel with nanofiber fabrication, primary neurospheres are obtained from DPSCs isolated from human dental pulp as schematized in Figure a. Intended to allow our study to start from a unique and well‐defined cell population that is then cultured on different classes of scaffolds, the intermediate neurosphere generation is associated with the induction of neuronal precommitment in the used cells . This will lead to remarkable results upon growth on some of the investigated scaffolds, as detailed in the following.…”

Section: Resultsmentioning

confidence: 99%

Lineage‐Specific Commitment of Stem Cells with Organic and Graphene Oxide–Functionalized Nanofibers

Portone

Moffa

Gardin

et al. 2018

Adv Funct Materials

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Controlling the differentiation to certain lineages is the main goal of current stem cell research, which might exploit new routes based on the interaction of cells with nanomaterials. Here it is shown that primary neurospheres from dental pulp stem cells grown on combinatorial surfaces with different fibrous morphology and graphene oxide functionalization exhibit different differentiation propensity. The developed materials strongly influence the stem cell fate, as highlighted by morphological, immunofluorescence, molecular biology, and functional analyses. Instructive cues lead to the increased expression of markers that are characteristic of selective differentiation into osteoblasts, glial cells, fibroblasts, and neurons even in basal medium conditions, and randomly oriented fibers are found to revert neuronal precommitment and to trigger osteoblastic differentiation. Graphene oxide coatings lead instead to the relatively enhanced expression of genes typical of either glial or neuronal commitment, depending on the underlying nanofibrous morphology. The mechanisms addressing cell fate are investigated, highlighting the correlation of wetting anisotropy and protein adsorption capacity of different surfaces, ultimate cell conformational changes reflected by skeletal and nuclear elongation, and directed cell commitment. Cues from the different surfaces are therefore lineage-specific, unveiling remarkable potentialities for cellular programming by means of nanomaterials.

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“…Dental pulp stem cells may thus be useful in oral and maxillofacial reconstruction (Rapino et al, 2019). During osteoblast differentiation, DPSCs express osteoblast differentiation-related biomarkers such as runt-related transcription factor 1 (RUNX1), osteocalcin (OCN), alkaline phosphatase (ALP), and osterix (OSX) (Sharpe, 2016;Victor and Reiter, 2017). Our previous studies have found that downregulation of nuclear factor erythroid 2 related factor promotes autophagy-dependent osteoblastic adipose-derived MSC differentiation (Tao et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

hsa_circ_0026827 Promotes Osteoblast Differentiation of Human Dental Pulp Stem Cells Through the Beclin1 and RUNX1 Signaling Pathways by Sponging miR-188-3p

Zhu

Pan

et al. 2020

Front. Cell Dev. Biol.

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Previous studies have found that circular RNA (circRNA) hsa_circ_0026827 plays a role during osteoblast differentiation, but the mechanism is unclear. The aim of this study was to illuminate the role of hsa_circ_0026827 in human dental pulp stem cells (DPSCs) during osteoblast differentiation. The results show that hsa_circ_0026827 expression significantly increased during osteoblast differentiation, while knockdown of hsa_circ_0026827 suppressed DPSC-derived osteoblast differentiation. microRNA (miRNA) expression profile analysis showed that downregulation of hsa_circ_0026827 promoted miR-188-3p expression. miR-188-3p downregulation restored osteogenic differentiation of DPSCs after hsa_circ_0026827 was silenced. Luciferase reporter assays verified that miR-188-3p was the target of hsa_circ_0026827 and also demonstrated that Beclin1 and RUNX1 were miR-188-3p downstream targets. miR-188-3p overexpression suppressed DPSC osteogenic differentiation by targeting Beclin-1-mediated autophagy and runt-related transcription factor 1 (RUNX1). In vivo studies using a heterotopic bone model also found that hsa_circ_0026827 overexpression plays an important role in promoting heterotopic bone formation. In conclusion, our research indicates that hsa_circ_0026827 promotes osteoblast differentiation of DPSCs via Beclin1 and the RUNX1 signaling pathways by sponging miR-188-3p, which suggests novel therapeutics for osteoporosis treatment.

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Dental pulp stem cells for the study of neurogenetic disorders

Cited by 40 publications

References 53 publications

Enhanced Skin Permeation of Anti-wrinkle Peptides via Molecular Modification

Enhanced Skin Permeation of Anti-wrinkle Peptides via Molecular Modification

Lineage‐Specific Commitment of Stem Cells with Organic and Graphene Oxide–Functionalized Nanofibers

hsa_circ_0026827 Promotes Osteoblast Differentiation of Human Dental Pulp Stem Cells Through the Beclin1 and RUNX1 Signaling Pathways by Sponging miR-188-3p

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