Dental Pulp-Derived Stem Cells Preserve Astrocyte Health During Induced Gliosis by Modulating Mitochondrial Activity and Functions

Barthels, Derek; Prateeksha, Prateeksha; Nozohouri, Saeideh; Villalba, Heidi; Zhang, Yong; Sharma, Sejal; Anderson, Steven J.; Howlader, Md Sariful Islam; Nambiar, Adarsh; Abbruscato, Thomas J.; Das, Hiranmoy

doi:10.1007/s10571-022-01291-8

Cited by 13 publications

(9 citation statements)

References 69 publications

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“…TIMPs can directly promote ECM deposition by inhibiting the MMPs, hence ECM proteolysis, and indirectly regulate ECM turnover ( 43 ). In the present study, it was observed that when DPSCs are exposed to stressful conditions, they secrete greater amounts of neurotrophic factors, such as BDNF, GDNF, as well as TIMP-2 which helps protect the tissue integrity by inhibiting MMP activity ( 44 ). Higher expressions of TIMP-2 were also observed in inflamed dental pulp compared to the healthy dental pulp ( 45 ).…”

Section: Discussionmentioning

confidence: 85%

The Effects of Grape Seed Oligomeric Proanthocyanidin and Nisin on Dental Pulp Stem Cells

Ballikaya,

Babadag,

Oya San Keskin

et al. 2024

Acta Stomatol Croat

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Objective This study aimed to evaluate the biological effects of “proanthocyanidin” (PA), and “nisin” (Ni), on dental pulp stem cells (DPSCs) and LPS-induced DPSCs as well as their antimicrobial effects against S. aureus and E. coli . Materials and methods After characterization of DPSCs, cytotoxicity of PA and Ni on DPSCs were evaluated using a water-soluble tetrazolium salt (WST-1). The cytokines and chemokines released by DPSCs and the expression levels of IL-6, IL-8, and TNF alpha were detected with human Cytokine Array C5 and enzyme‐linked immunosorbent assay (ELİSA), respectively. The antibacterial activities of PA and Ni were tested using the drop plate method. Results PA at 75 μg/ml increased cell viability, decreased TNF-α expression of DPSCs, did not show any cytotoxic effects on LPS-induced DPSCs, and also showed a tendency to decrease TNF-α expression. PA at 75 μg/ml exhibited higher expressions of TIMP-2, OPG, IL-7, and IL-8 in LPS-induced DPSCs compared to DPSCs. Ni at 100 μg/ml decreased TNF-α expression in DPSCs with no cytotoxic effects. It provided increased cell viability and a downregulation trend of TNF-α expression in LPS-induced DPSCs. Both Ni and PA provided strong antibacterial effects against S. aureus . Ni at 200μg/ml had strong antibacterial effects against E. coli without affecting negatively the viability of both DPSCs and LPS-induced DPSCs and showed anti-inflammatory activity by decreasing TNF-α expression. PA provided strong antibacterial effects against E. coli at 200 μg/ml but affected DPSCs viability negatively. Conclusion PA and Ni at specific concentrations exhibited immunomodulatory activity on DPSCs and LPS-induced DPSCs without any cytotoxic effects and strong antibacterial effects on S. aureus .

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Section: Discussionmentioning

confidence: 85%

The Effects of Grape Seed Oligomeric Proanthocyanidin and Nisin on Dental Pulp Stem Cells

Ballikaya,

Babadag,

Oya San Keskin

et al. 2024

Acta Stomatol Croat

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“…Autophagy processes can recycle cytoplasmic organelles and components for eventual tissue remodeling [37,69,86,87,175,177,[207][208][209][210][211][212][213][214][215][216]. There are subtypes of autophagy processing.…”

Section: Cellular Metabolism and The Role Of Apoptosis Autophagy And ...mentioning

confidence: 99%

Cellular Metabolism: A Fundamental Component of Degeneration in the Nervous System

Maiese

2023

Biomolecules

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It is estimated that, at minimum, 500 million individuals suffer from cellular metabolic dysfunction, such as diabetes mellitus (DM), throughout the world. Even more concerning is the knowledge that metabolic disease is intimately tied to neurodegenerative disorders, affecting both the central and peripheral nervous systems as well as leading to dementia, the seventh leading cause of death. New and innovative therapeutic strategies that address cellular metabolism, apoptosis, autophagy, and pyroptosis, the mechanistic target of rapamycin (mTOR), AMP activated protein kinase (AMPK), growth factor signaling with erythropoietin (EPO), and risk factors such as the apolipoprotein E (APOE-ε4) gene and coronavirus disease 2019 (COVID-19) can offer valuable insights for the clinical care and treatment of neurodegenerative disorders impacted by cellular metabolic disease. Critical insight into and modulation of these complex pathways are required since mTOR signaling pathways, such as AMPK activation, can improve memory retention in Alzheimer’s disease (AD) and DM, promote healthy aging, facilitate clearance of β-amyloid (Aß) and tau in the brain, and control inflammation, but also may lead to cognitive loss and long-COVID syndrome through mechanisms that can include oxidative stress, mitochondrial dysfunction, cytokine release, and APOE-ε4 if pathways such as autophagy and other mechanisms of programmed cell death are left unchecked.

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“…Nervous system diseases comprise a large portion of NCDs [2,[29][30][31][32][33][34][35][36][37][38][39][40]. Neurodegenerative disorders impact more than fifteen percent of the global population and comprise greater than six hundred disease entities that can result in severe disability and death [10,37,[41][42][43][44][45][46][47].…”

Section: Increased Lifespan Aging Cellular Senescence and Nervous Sys...mentioning

confidence: 99%

“…Recent studies have identified a significant role for pathways of programmed cell death that can ultimately control cell survival and cognitive impairment during MS [46,61,128,130,132,134,[200][201][202] (Table 1). Programmed cell death pathways that involve autophagy, apoptosis, pyroptosis, and ferroptosis can influence cell survival during inflammation [34,39,66,73,74,85,87,[203][204][205][206][207][208][209][210][211][212][213], oxidative stress [10,45,77,86,89,93,119,185,[214][215][216][217][218][219], ischemia [219][220][221][222][223], and mitochondrial dysfunction [32,…”

Section: Autophagy Apoptosis Pyroptosis and Ferroptosis Involvement I...mentioning

confidence: 99%

Cognitive Impairment in Multiple Sclerosis

Maiese

2023

Bioengineering

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Almost three million individuals suffer from multiple sclerosis (MS) throughout the world, a demyelinating disease in the nervous system with increased prevalence over the last five decades, and is now being recognized as one significant etiology of cognitive loss and dementia. Presently, disease modifying therapies can limit the rate of relapse and potentially reduce brain volume loss in patients with MS, but unfortunately cannot prevent disease progression or the onset of cognitive disability. Innovative strategies are therefore required to address areas of inflammation, immune cell activation, and cell survival that involve novel pathways of programmed cell death, mammalian forkhead transcription factors (FoxOs), the mechanistic target of rapamycin (mTOR), AMP activated protein kinase (AMPK), the silent mating type information regulation 2 homolog 1 (Saccharomyces cerevisiae) (SIRT1), and associated pathways with the apolipoprotein E (APOE-ε4) gene and severe acute respiratory syndrome coronavirus (SARS-CoV-2). These pathways are intertwined at multiple levels and can involve metabolic oversight with cellular metabolism dependent upon nicotinamide adenine dinucleotide (NAD+). Insight into the mechanisms of these pathways can provide new avenues of discovery for the therapeutic treatment of dementia and loss in cognition that occurs during MS.

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Dental Pulp-Derived Stem Cells Preserve Astrocyte Health During Induced Gliosis by Modulating Mitochondrial Activity and Functions

Cited by 13 publications

References 69 publications

The Effects of Grape Seed Oligomeric Proanthocyanidin and Nisin on Dental Pulp Stem Cells

The Effects of Grape Seed Oligomeric Proanthocyanidin and Nisin on Dental Pulp Stem Cells

Cellular Metabolism: A Fundamental Component of Degeneration in the Nervous System

Cognitive Impairment in Multiple Sclerosis

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