Dental Plaque Microbial Resistomes of Periodontal Health and Disease and Their Changes after Scaling and Root Planing Therapy

Kang, Yutong; Sun, Bianjin; Chen, Yi–Ju; Lou, Yongliang; Zheng, Meiqin; Li, Zhenjun

doi:10.1128/msphere.00162-21

Cited by 17 publications

(23 citation statements)

References 84 publications

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“…Other studies have shown that clinical improvement post SRP was accompanied by changes in the subgingival microbiota (Haffajee et al, 1997; Feres et al, 2020) and reduction in members of the red complex (Belstrøm et al, 2018; Kang et al, 2021). We did not observe a decrease in those organisms after treatment; it may be because, in our case, we were measuring the active community and not just the metagenome.…”

Section: Discussionmentioning

confidence: 95%

Subgingival host–microbiome metatranscriptomic changes following scaling and root planing in grade II/III periodontitis

Duran-Pinedo

Solbiati

Teles

et al. 2022

J Clinic Periodontology

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Aim To assess the effects of scaling and root planing (SRP) on the dynamics of gene expression by the host and the microbiome in subgingival plaque samples. Materials and Methods Fourteen periodontitis patients were closely monitored in the absence of periodontal treatment for 12 months. During this period, comprehensive periodontal examination and subgingival biofilm sample collection were performed bi‐monthly. After 12 months, clinical attachment level (CAL) data were compiled and analysed using linear mixed models (LMM) fitted to longitudinal CAL measurements for each tooth site. LMM classified the sites as stable (S), progressing (P), or fluctuating (F). After the 12‐month visit, subjects received SRP, and at 15 months they received comprehensive examination and supportive periodontal therapy. Those procedures were repeated at the 18‐month visit, when patients were also sampled. Each patient contributed with one S, one P, and one F site collected at the 12‐ and 18‐month visits. Samples were analysed using Dual RNA‐Sequencing to capture host and bacterial transcriptomes simultaneously. Results Microbiome and host response behaviour were specific to the site's progression classification (i.e., S, P, or F). Microbial profiles of pre‐ and post‐treatment samples exhibited specific microbiome changes, with progressing sites showing the most significant changes. Among them, Porphyromonas gingivalis was reduced after treatment, while Fusobacterium nucleatum showed an increase in proportion. Transcriptome analysis of the host response showed that interleukin (IL)‐17, TNF signalling pathways, and neutrophil extracellular trap formation were the primary immune response activities impacted by periodontal treatment. Conclusions SRP resulted in a significant “rewiring” of host and microbial activities in the progressing sites, while restructuring of the microbiome was minor in stable and fluctuating sites.

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Section: Discussionmentioning

confidence: 95%

Subgingival host–microbiome metatranscriptomic changes following scaling and root planing in grade II/III periodontitis

Duran-Pinedo

Solbiati

Teles

et al. 2022

J Clinic Periodontology

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“…With this background, it is pertinent to emphasize that a current investigation denoted that antimicrobial-resistance genes were perceived in the plaque of more than 50% of healthy persons [ 45 ]. This aspect can partially elucidate the decreasing efficacy of the antimicrobials implemented clinically [ 46 ].…”

Section: Discussionmentioning

confidence: 99%

Antibiotic Resistance in Patients with Peri-Implantitis: A Systematic Scoping Review

Medina

Vivares-Builes

2022

IJERPH

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The implementation of adjunctive antibiotics has been recommended for the therapy of peri-implantitis (PI). In this review, antibiotic resistance patterns in PI patients were assessed. A systematic scoping review of observational studies and trials was established in conjunction with the PRISMA extension for scoping reviews. The SCOPUS, PubMed/MEDLINE, EMBASE, SCIELO, Web of Science, and LILACS databases were reviewed along with the gray literature. The primary electronic examination produced 139 investigations. Finally, four observational studies met the selection criteria. These studies evaluated 214 implants in 168 patients. Porphyromonas gingivalis and Fusobacterium nucleatum mainly presented high resistance to tetracycline, metronidazole, and erythromycin in PI patients. Similarly, Aggregatibacter actinomycetemcomitans was also highly resistant to clindamycin and doxycycline. Other microorganisms such as Tannerella forsythia, Parvimonas micra, and Prevotella intermedia/nigrescens also presented significant levels of resistance to other antibiotics including amoxicillin, azithromycin, and moxifloxacin. However, most microorganisms did not show resistance to the combination amoxicillin metronidazole. Although the management of adjunctive antimicrobials in the therapy of PI is controversial, in this review, the resistance of relevant microorganisms to antibiotics used to treat PI, and usually prescribed in dentistry, was observed. Clinicians should consider the antibiotic resistance demonstrated in the treatment of PI patients and its public health consequences.

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“…AA induces an inhibition zone on periodonpathogenic bacterial cultures ( Saini et al, 2008 , Tambekar et al, 2009 ) and suppresses P. gingivalis and P. intermedia ( Clark et al, 1993 ). A recent genetic analysis has revealed emerging antibiotic-resistant genes (ARGs) and metal-resistant genes (MRGs) in the dental plaque microbiota after scaling and root planing, thus highlighting the need for novel antibacterial strategies to control periodontitis ( Kang et al, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

Effect of Gum Arabic on plaque-induced gingivitis: A randomised controlled trial

Gafar

Ramadan

ElSaid³

et al. 2022

The Saudi Dental Journal

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Dental Plaque Microbial Resistomes of Periodontal Health and Disease and Their Changes after Scaling and Root Planing Therapy

Cited by 17 publications

References 84 publications

Subgingival host–microbiome metatranscriptomic changes following scaling and root planing in grade II/III periodontitis

Subgingival host–microbiome metatranscriptomic changes following scaling and root planing in grade II/III periodontitis

Antibiotic Resistance in Patients with Peri-Implantitis: A Systematic Scoping Review

Effect of Gum Arabic on plaque-induced gingivitis: A randomised controlled trial

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