Dental implant surface chemistry and energy alter macrophage activation <i>in vitro</i>

Hotchkiss, Kelly; Ayad, Nancy B.; Hyzy, Sharon L.; Boyan, Barbara D.; Olivares-Navarrete, René

doi:10.1111/clr.12814

Cited by 83 publications

(88 citation statements)

References 39 publications

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“…Furthermore, this is the first reported use of these transcortical implants custom‐made to fit rat femurs. Scanning electron microscopy, laser confocal microscopy, and x‐ray photoelectron spectroscopy revealed a surface morphology, average surface roughness, and surface composition similar to what has been previously reported for implants processed in the same manner …”

Section: Discussionsupporting

confidence: 81%

Ibandronate Treatment Before and After Implant Insertion Impairs Osseointegration in Aged Rats with Ovariectomy Induced Osteoporosis

et al. 2019

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Excessive decreases in bone volume (BV) and bone mineral density (BMD) can lead to osteoporosis, potentially hindering implant osseointegration. Bisphosphonates are commonly used to combat osteoporosis by slowing osteoclast‐mediated resorption; however, functional osteoclasts are integral to bone remodeling and, thus, implant osseointegration, potentially contraindicating bisphosphonate use during implantation. To optimize the use of implant technologies in patients with compromised bone structure and metabolism, we need a more complete understanding of the biological response to surface design. The goal of this study was to assess the effects of osteoporosis and bisphosphonates on osseointegration of titanium (Ti) implants with microstructured surfaces, which have been shown to support osteoblast differentiation in vitro and rapid osseointegration in vivo . Forty, 8‐month‐old, virgin, female CD Sprague Dawley rats underwent ovariectomy (OVX) or sham (SHOVX) surgery. After 5 weeks, animals were injected subcutaneously with either the bisphosphonate (BIS), Ibandronate (25 µg/kg), or phosphate‐buffered saline (PBS) every 25 days. 1 week after the initial injection, Ø2.5mm × 3.5mm microrough (SLA; grit‐blasted/acid etched) implants were placed transcortically in the distal metaphysis of each femur resulting in four groups: 1) SHOVX+PBS; 2) SHOVX+BIS; 3) OVX+PBS; and 4) OVX+BIS. After 28d, qualitative properties of the bone and implant osseointegration were assessed using micro‐computed tomography (microCT), calcified histomorphometry (Van Gieson's stain), and removal torque testing. microCT revealed decreased bone volume in OVX rats, which was slowed by bisphosphonate treatment. Reduced bone‐to‐implant contact (BIC) was evident in OVX+PBS compared to SHOVX+PBS. Although BV/TV was increased in OVX+BIS compared to OVX+PBS, bisphosphonate treatment had no effect on BIC. Removal torque testing revealed a higher maximum torque, torsional stiffness, and torsional energy in SHOVX compared to OVX with no effects due to bisphosphonate treatment. Our results show that osseointegration is decreased in osteoporotic animals. Ibandronate halts the progression of osteoporosis but does not enhance osseointegration. © 2019 The Authors. JBMR Plus Published by Wiley Periodicals, Inc. on behalf of American Society for Bone and Mineral Research

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Section: Discussionsupporting

confidence: 81%

Ibandronate Treatment Before and After Implant Insertion Impairs Osseointegration in Aged Rats with Ovariectomy Induced Osteoporosis

et al. 2019

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“…At the micron level, blasting and etching produces a severely pitted surface and subsequent storage in saline not only results in a hydrophilic surface (modSLA), but also the spontaneous formation of nanoscale sized surface structures (Wennerberg, Svanborg, Berner, & Andersson, ). These features have been shown by our group (Alfarsi et al, ; Hamlet et al, ) and others (Hotchkiss et al, , ; Lu & Webster, ; Lü et al, ) to affect both the attachment of monocytes and macrophages, as well as the gene expression and secretion of pro‐inflammatory cytokines.…”

Section: Discussionsupporting

confidence: 73%

“…Biomaterial features such as topography, particle size, porosity and ion release are all biomaterial-specific factors able to influence the immune response. Hydrophobic materials, for example, have been shown to enhance monocyte adhesion (Hezi-Yamit et al, 2009), whereas hydrophilic or neutral surfaces can inhibit macrophage adhesion but enhance the release of pro-inflammatory cytokines and chemokines (Alfarsi et al, 2014;Hamlet et al, 2012;Hotchkiss, Ayad, Hyzy, Boyan, & Olivares-Navarrete, 2017;Hotchkiss et al, 2016;Jones et al, 2007). More recently, a hydrophilic titanium surface with nanotopography (modSLA) has been shown to modulate the early inflammatory response in vivo promoting polarization of macrophages to an M2-like phenotype, even in diabetic conditions where an exaggerated pro-inflammatory environment is a distinguishing feature (Lee et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

Hydrophilic titanium surface‐induced macrophage modulation promotes pro‐osteogenic signalling

Hamlet

Lee

Moon

et al. 2019

Clinical Oral Implants Res

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Objectives As biomaterial‐induced modulation of mediators of the immune response may be a potential therapeutic approach to enhance wound healing events, the aim of this study was to delineate the effects of titanium surface modification on macrophage phenotype and function. Material and methods Rodent bone marrow‐derived macrophages were polarized into M1 and M2 phenotypes and cultured on micro‐rough (SLA) and hydrophilic modified SLA (modSLA) titanium discs. Macrophage phenotype and cytokine secretion were subsequently assessed by immunostaining and ELISA, respectively. Osteoblast gene expression in response to culture in the M1 and M2 macrophage conditioned media was also evaluated over 7 days by RT‐PCR. Results M1 macrophage culture on the modSLA surface promoted an M2‐like phenotype as demonstrated by marked CD163 protein expression, Arg1 gene expression and the secretion of cytokines that significantly upregulated in osteoblasts the expression of genes associated with the TGF‐ß/BMP signalling pathway and osteogenesis. In comparison, M2 macrophage culture on SLA surface promoted an inflammatory phenotype and cytokine profile that was not conducive for osteogenic gene expression. Conclusions Macrophages are able to alter or switch their phenotype according to the signals received from the biomaterial surface. A hydrophilic micro‐rough titanium surface topography elicits a macrophage phenotype associated with reduced inflammation and enhanced pro‐osteogenic signalling.

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“…It is now understood that surface properties modulate immune cell function and factors produced by macrophages influence osteogenesis [26, 27]. Others have shown that osteocytes make direct contact with the implant surface, suggesting their importance in maintaining healthy bone remodeling as well as the longevity of implant osseointegration [28, 29].…”

Section: Discussionmentioning

confidence: 99%

Regulation of osteoclasts by osteoblast lineage cells depends on titanium implant surface properties

Lotz

Berger

Schwartz³

et al. 2018

Acta Biomaterialia

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In this study, we developed a novel in vitro model to study how microstructured and hydrophilic titanium implants impact bone remodeling for dental and orthopaedic applications. Our approach intersects biomaterials and systems physiology, revealing for the first time that implant surface properties are capable of regulating the communication among the cells involved in remodeling of primary bone during osseointegration. We believe that the basic research presented in our manuscript will provide important knowledge in our understanding of factors that impact implant success. Furthermore, it provides a solid foundation for the development of materials that enable rapid osseointegration and earlier loading times for implants in bone that has been compromised by trauma or disease.

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Dental implant surface chemistry and energy alter macrophage activation in vitro

Abstract: The combination of high-energy and altered surface chemistry present on TiZr modSLA was able to influence macrophages to produce the greatest anti-inflammatory microenvironment and reduce extended pro-inflammatory factor release.

Cited by 83 publications

References 39 publications

Ibandronate Treatment Before and After Implant Insertion Impairs Osseointegration in Aged Rats with Ovariectomy Induced Osteoporosis

Ibandronate Treatment Before and After Implant Insertion Impairs Osseointegration in Aged Rats with Ovariectomy Induced Osteoporosis

Hydrophilic titanium surface‐induced macrophage modulation promotes pro‐osteogenic signalling

Regulation of osteoclasts by osteoblast lineage cells depends on titanium implant surface properties

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