Dental caries in gnotobiotic rats infected with a variety of Lactobacillus acidophilus

Fitzgerald, Robert J.; Jordan, H.V.; Archard, H.O.

doi:10.1016/0003-9969(66)90153-1

Cited by 58 publications

(17 citation statements)

References 11 publications

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“…into gnotobiotic rats (17,44) but are normally considered to be associated with the progression of caries (48,51). Lactobacilli have been previously detected in carious dentin (8,10,29).…”

Section: Vol 48 2010 Sequence Analysis Of Severe Caries In Permanenmentioning

confidence: 99%

Bacterial 16S Sequence Analysis of Severe Caries in Young Permanent Teeth

et al. 2010

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Previous studies have confirmed the association of the acid producers Streptococcus mutans and Lactobacillus spp. with childhood caries, but they also suggested these microorganisms are not sufficient to explain all cases of caries. In addition, health-associated bacterial community profiles are not well understood, including the importance of base production and acid catabolism in pH homeostasis. The bacterial community composition in health and in severe caries of the young permanent dentition was compared using Sanger sequencing of the ribosomal 16S rRNA genes. Lactobacillus species were dominant in severe caries, and levels rose significantly as caries progressed from initial to deep lesions. S. mutans was often observed at high levels in the early stages of caries but also in some healthy subjects and was not statistically significantly associated with caries progression in the overall model. Lactobacillus or S. mutans was found either at low levels or not present in several samples. Other potential acid producers observed at high levels in these subjects included strains of Selenomonas, Neisseria, and Streptococcus mitis. Propionibacterium FMA5 was significantly associated with caries progression but was not found at high levels. An overall loss of community diversity occurred as caries progressed, and species that significantly decreased included the Streptococcus mitis-S. pneumoniae-S. infantis group, Corynebacterium matruchotii, Streptococcus gordonii, Streptococcus cristatus, Capnocytophaga gingivalis, Eubacterium IR009, Campylobacter rectus, and Lachnospiraceae sp. C1. The relationship of acid-base metabolism to 16S rRNA gene-based species assignments appears to be complex, and metagenomic approaches that would allow functional profiling of entire genomes will be helpful in elucidating the microbial pathogenesis of caries.Dental caries is the most common chronic disease of childhood, affecting nearly three-fourths of all children by the age of 17 years (50). The majority of children experience mild caries in the permanent dentition that is easily managed, but nearly 20% of children suffer more aggressive caries (19) that is destructive and often recurrent. The cariogenicity of Streptococcus mutans and Lactobacillus species in tooth-associated biofilms has long been established based on culture studies (51), but this approach has provided a limited ability to study the role of other species present in biofilm communities. Recently DNA-based methods have been used to study early childhood caries (5, 11, 23), caries of the primary and permanent teeth in children and young adults (1), root caries in the elderly (42), and advanced dentin lesions (8,10,36). Taken together these studies have confirmed the association of S. mutans and Lactobacillus species with childhood caries, but they also suggest that these species are not sufficient to explain all cases of caries. In addition, health-associated bacterial community profiles are not well understood, including the importance of species that produce basic co...

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“…into gnotobiotic rats (17,44) but are normally considered to be associated with the progression of caries (48,51). Lactobacilli have been previously detected in carious dentin (8,10,29).…”

Section: Vol 48 2010 Sequence Analysis Of Severe Caries In Permanenmentioning

confidence: 99%

Bacterial 16S Sequence Analysis of Severe Caries in Young Permanent Teeth

et al. 2010

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“…Induction of caries infected with lactobacilli in gnotobiotic rats had also been reported by Fitzgerald et al [3] and Rosen et al [10]. From this view point, a study was conducted to determine whether, and if at all, how and to what extent the distribution of iodophilic polysaccharide-synthesizing lactobacilli might be related to the incidence of development of dental caries.…”

mentioning

confidence: 77%

Distribution of Iodophilic Polysaccharide Synthesizing Lactobacilli in Oral Cavities

Kobayashi

Takei

1972

Japanese Journal of Microbiology

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“…Serological groups among lactobacilli (Sharpe, 1955(Sharpe, , 1970 correspond to physiologically identifiable species. It should be emphasised that Fitzgerald (1 963) was unable to induce caries in gnotobiotic rats with either L. acidophilus or L. fermenturn, though a variant of L. acidophilus produced fissure caries (Fitzgerald, Jordan and Archard, 1966).…”

Section: Discussionmentioning

confidence: 99%