Density of Vibrios in Hemolymph and Hepatopancreas of Diseased Pacific White Shrimp, <i>Litopenaeus vannamei</i>, from Northwestern Mexico

Soto-Rodríguez, Sonia A.; Gil, Bruno Gómez; Lozano, Rodolfo; Roque, Ana

doi:10.1111/j.1749-7345.2009.00335.x

Cited by 31 publications

(26 citation statements)

References 20 publications

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“…Mexican shrimp production depends mainly on the northwestern states, and Sonora, Sinaloa, Nayarit, and Baja California reported a joint production in 2011 of 105,218 tons, which was worth 4.263 billion Mexican pesos (40) (http://www.conapesca.sagarpa.gob.mx/wb/cona/anuario_2011). Unfortunately, the rapid growth of this industry has increased the number of epizootics associated with infectious diseases in Mexico and other countries in Latin America, and significant losses of shrimp production have occurred because of disease caused by viruses, such as infectious hypodermal and hematopoietic necrosis virus (IHHNV), Taura syndrome virus (TSV), and white spot syndrome virus (WSSV) (1), and bacteria, such as the necrotizing hepatopancreatitis bacterium (NHP-B) (2,3) and Vibrio (4). Vibriosis is a disease caused by bacteria belonging to the Vibrio genus that produce necrotic lesions in the tissues of infected organisms (5,6).…”

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confidence: 99%

Field and Experimental Evidence of Vibrio parahaemolyticus as the Causative Agent of Acute Hepatopancreatic Necrosis Disease of Cultured Shrimp (Litopenaeus vannamei) in Northwestern Mexico

Soto-Rodríguez

Gómez‐Gil

Lozano‐Olvera

et al. 2015

Appl Environ Microbiol

306

212

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Moribund shrimp affected by acute hepatopancreatic necrosis disease (AHPND) from farms in northwestern Mexico were sampled for bacteriological and histological analysis. Bacterial isolates were molecularly identified as Vibrio parahaemolyticus by the presence of the tlh gene. The tdh-negative, trh-negative, and tlh-positive V. parahaemolyticus strains were further characterized by repetitive extragenic palindromic element-PCR (rep-PCR), and primers AP1, AP2, AP3, and AP and an ems2 IQ2000 detection kit (GeneReach, Taiwan) were used in the diagnostic tests for AHPND. The V. parahaemolyticus strains were used in immersion challenges with shrimp, and farmed and challenged shrimp presented the same clinical and pathological symptoms: lethargy, empty gut, pale and aqueous hepatopancreas, and expanded chromatophores. Using histological analysis and bacterial density count, three stages of AHNPD (initial, acute, and terminal) were identified in the affected shrimp. The pathognomonic lesions indicating severe desquamation of tubular epithelial cells of the hepatopancreas were observed in both challenged and pond-infected shrimp. The results showed that different V. parahaemolyticus strains have different virulences; some of the less virulent strains do not induce 100% mortality, and mortality rates also rise more slowly than they do for the more virulent strains. The virulence of V. parahaemolyticus strains was dose dependent, where the threshold infective density was 10 4 CFU ml ؊1 ; below that density, no mortality was observed. The AP3 primer set had the best sensitivity and specificity. Field and experimental results showed that the V. parahaemolyticus strain that causes AHPND acts as a primary pathogen for shrimp in Mexico compared with the V. parahaemolyticus strains reported to date.

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mentioning

confidence: 99%

Field and Experimental Evidence of Vibrio parahaemolyticus as the Causative Agent of Acute Hepatopancreatic Necrosis Disease of Cultured Shrimp (Litopenaeus vannamei) in Northwestern Mexico

Soto-Rodríguez

Gómez‐Gil

Lozano‐Olvera

et al. 2015

Appl Environ Microbiol

306

212

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“…Whilst WSSV was sometimes detected during outbreaks of BRS, vibriosis was always present with the CAIM 1792 strain predominating in the haemolymph of all shrimp sampled. The density of vibrio in haemolymph and the hepatopancreas was similar to Litopenaeus vananmei shrimp with vibriosis cultured in northwestern Mexico (Soto-Rodriguez et al 2010).…”

Section: Discussionmentioning

confidence: 81%

“…The minimum inhibitory concentration (MIC) was determined for 29 bacterial isolates in Müller-Hinton broth containing 2.5% NaCl using a microdilution method (Rangdale et al 1997, Soto-Rodriguez et al 2010. Dominant yellow colonies picked from the TCBS plates were used to prepare a bacterial suspension to determine the MIC of oxytetracycline (OTC), enrofloxacine (ENR), florfenicol (FLO), and norfloxacine (NOR).…”

Section: Methodsmentioning

confidence: 99%

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‘Bright-red’ syndrome in Pacific white shrimp Litopenaeus vannamei is caused by Vibrio harveyi

Soto-Rodríguez¹,

Gómez‐Gil²,

Lozano³

2010

Dis. Aquat. Org.

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Since July 2005, recurrent outbreaks of vibriosis have occurred in shrimp farms in northwestern Mexico. Moribund Litopenaeus vannamei associated with mass mortalities were lethargic and displayed red discoloration spots on their abdomen, and hence were called 'bright-reds' by farmers. Shrimp submitted for diagnosis were examined using wet tissue mounts, bacteriological assays and their respective minimum inhibitory concentration (MIC), and histology. A dominant yellow bacterial colony was isolated in thiosulphate citrate bile salts-sucrose (TCBS) agar and identified by molecular methods as Vibrio harveyi strain CAIM 1792. Pathogenicity of the V. harveyi strain was demonstrated in L. vannamei. The lowest MIC against Vibrio isolates from bright-red shrimp was obtained with enrofloxacine (3.01, SD = 5.96 µg ml

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“…The relationship between weight and microbiota of Vibrio in shrimp hepatopancreas has been reported by Soto Rodríguez et al (9). In Shrimp Diseases, the authors cited found that vibrio density in hepatopancreas individuals weighing 8-12 g had a greater bacterial loads than individuals weighing 0.26-4.0 g.…”

Section: Discussionmentioning

confidence: 84%

Microbiota of Vibrio sp. in the hepatopancreas of cultured white pacific shrimp (Litopenaeus vannamei)

et al. 2013

Rev MVZ Córdoba

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ORIGINAL Microbiota of ABSTRACTObjective. The present study aimed to investigate the presence of vibrios in the hepatopancreas of cultured shrimp. Materials and methods. Vibrios from the hepatopancreas of fifteen samples of five specimens each, of apparently healthy Pacific white shrimp (Litopenaeus vannamei) were isolated, identified and quantified. Results. The vibrio density ranged from 430 to 2,400 MPN g -1 (r s MPN cm -1 =-0.114; r s MPN g -1 = 0.211). Thirty isolations were obtained, most of which belonged to the species V. cholerae (n=11) and V. parahaemolyticus (n=7). Conclusions. The outcomes of the present study suggest that, even in the absence of symptoms of vibriosis, the microbiota of the hepatopancreas of cultured shrimp may include sucrose positive and negative vibrios.

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Density of Vibrios in Hemolymph and Hepatopancreas of Diseased Pacific White Shrimp, Litopenaeus vannamei, from Northwestern Mexico

Cited by 31 publications

References 20 publications

Field and Experimental Evidence of Vibrio parahaemolyticus as the Causative Agent of Acute Hepatopancreatic Necrosis Disease of Cultured Shrimp (Litopenaeus vannamei) in Northwestern Mexico

Field and Experimental Evidence of Vibrio parahaemolyticus as the Causative Agent of Acute Hepatopancreatic Necrosis Disease of Cultured Shrimp (Litopenaeus vannamei) in Northwestern Mexico

‘Bright-red’ syndrome in Pacific white shrimp Litopenaeus vannamei is caused by Vibrio harveyi

Microbiota of Vibrio sp. in the hepatopancreas of cultured white pacific shrimp (Litopenaeus vannamei)

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