Density of a sample bovine cortical bone matrix and its solid constituent in various media

Lees, Sidney; Heeley, J.D.

doi:10.1007/bf02409480

Cited by 25 publications

(16 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The dimensional changes of cortical bone matrix between the wet and dry states [10] closely matched the values reported by Ciferri et al [11] and others for rat tail tendon after cross-linking was induced with p-benzoquinone. values for tail tendon [7] under comparable physical conditions.…”

Section: Order and Orientation Of Bovine Cortical Bone Collagensupporting

confidence: 80%

“…The several regimes for water in collagen correspond to a gradation from water tightly bound within the molecular core through stages of successively weaker binding to free water in the 43% of the total matrix volume represented by L [10] (regime V). Presumably the water closer to the triple helix is more highly organized and there is a gradation of structure corresponding to the gradation of binding energy.…”

Section: Distribution Of Water In Bone Collagen Of the Wet Matrixmentioning

confidence: 99%

“…In the determination of the density of the dry bone matrix [10], it was concluded that all radial space, both inter-and intrafibrillar, was eliminated by dehydration. The X-ray diffraction patterns seem to confirm this conclusion.…”

Section: Order and Orientation Of Bovine Cortical Bone Collagenmentioning

confidence: 99%

“…The density of S in 0.15N saline was 1.33 g/cc. When the matrix was desiccated at 23~ in vacuum, the density of S was 1.42 g/cc [10].…”

Section: Order and Orientation Of Bovine Cortical Bone Collagenmentioning

confidence: 99%

“…The proportion in each of the three categories was based on the estimates of Katz and Li [3]. Recent work [7,9,10] provides a basis for a new estimate of the microcrystallite distribution together with a fresh attempt to model the bone collagen lattice and to describe the distribution of water in cortical bone and the bone matrix.…”

mentioning

confidence: 99%

See 4 more Smart Citations

A mixed packing model for bone collagen

Lees

1981

Calcif Tissue Int

Self Cite

View full text Add to dashboard Cite

A wide variety of physical properties, including sonic velocity, dimensional changes between wet and dried stages, anisotropy of the tissue properties, density, X-ray diffraction, differential microcalorimetry, dielectric constant, and composition (water, mineral, organic content) for the mineralized and demineralized tissue was used to develop a model for the superlattice structure of bone collagen. A mixed model is suggested where the collagen molecules are in register as in SLS type of aggregation within the microfibril, and the microfibrils are staggered in D unit steps according to the Hodge-Petruska scheme. A square packing model with 4 or more molecules per microfibril best fits the HP scheme with the effective molecular diameter of the wet collagen molecule, and allows for the regular array of axial gap filling microcrystallites of 5 nm or larger diameter. It is concluded that: 1. Macroscopic dimensional changes of adult bovine bone matrix closely match molecular dimensional changes of collagen superlattice. 2. Effective molecular diameter of dry collagen is 1.09 nm and that of wet bone collagen is 1.42-1.45 nm. 3. Water layer of the wet bone collagen molecule is 0.16 nm thick. 4. Water in the bone collagen molecule is distributed in 5 regimes much like in the tendon collagen molecule. 5. "Hidden" water, 0.10 g water per dry collagen of regimes I and II, is within the triple helix. 6. "External" water incorporated in the collagen molecule provides transition between the highly structured collagen molecule and the intermolecular medium. 7. Water incorporated in the mineralized bone collagen molecule is less than in demineralized bone matrix. 8. For adult bovine cortical bone, 25% by volume is water, 32% dry organic, 43% mineral; 28% by volume of the mineral is axial gap filling, 58% radial intrafibrillar, and 14% radial extrafibrillar.

show abstract

Section: Order and Orientation Of Bovine Cortical Bone Collagensupporting

confidence: 80%

Section: Distribution Of Water In Bone Collagen Of the Wet Matrixmentioning

confidence: 99%

Section: Order and Orientation Of Bovine Cortical Bone Collagenmentioning

confidence: 99%

“…The density of S in 0.15N saline was 1.33 g/cc. When the matrix was desiccated at 23~ in vacuum, the density of S was 1.42 g/cc [10].…”

Section: Order and Orientation Of Bovine Cortical Bone Collagenmentioning

confidence: 99%

mentioning

confidence: 99%

See 3 more Smart Citations

A mixed packing model for bone collagen

Lees

1981

Calcif Tissue Int

Self Cite

View full text Add to dashboard Cite

show abstract

Diagenetic Alterations to Vertebrate Mineralized Tissues – A Critical Review

Turner‐Walker¹

2023

Handbook of Archaeological Sciences

View full text Add to dashboard Cite

Subcanalicular Nanochannel Volume Is Inversely Correlated With Calcium Content in Human Cortical Bone

Tang

Landis

Blouin

et al. 2020

Journal of Bone and Mineral Research

View full text Add to dashboard Cite

The spatial distribution of mineralization density is an important signature of bone growth and remodeling processes, and its alterations are often related to disease. The extracellular matrix of some vertebrate mineralized tissues is known to be perfused by a lacunocanalicular network (LCN), a fluid‐filled unmineralized structure that harbors osteocytes and their fine processes and transports extracellular fluid and its constituents. The current report provides evidence for structural and compositional heterogeneity at an even smaller, subcanalicular scale. The work reveals an extensive unmineralized three‐dimensional (3D) network of nanochannels (~30 nm in diameter) penetrating the mineralized extracellular matrix of human femoral cortical bone and encompassing a greater volume fraction and surface area than these same parameters of the canaliculi comprising the LCN. The present study combines high‐resolution focused ion beam‐scanning electron microscopy (FIB‐SEM) to investigate bone ultrastructure in 3D with quantitative backscattered electron imaging (qBEI) to estimate local bone mineral content. The presence of nanochannels has been found to impact qBEI measurements fundamentally, such that volume percentage (vol%) of nanochannels correlates inversely with weight percentage (wt%) of calcium. This mathematical relationship between nanochannel vol% and calcium wt% suggests that the nanochannels could potentially provide space for ion and small molecule transport throughout the bone matrix. Collectively, these data propose a reinterpretation of qBEI measurements, accounting for nanochannel presence in human bone tissue in addition to collagen and mineral. Further, the results yield insight into bone mineralization processes at the nanometer scale and present the possibility for a potential role of the nanochannel system in permitting ion and small molecule diffusion throughout the extracellular matrix. Such a possible function could thereby lead to the sequestration or occlusion of the ions and small molecules within the extracellular matrix. © 2022 The Authors. Journal of Bone and Mineral Research published by Wiley Periodicals LLC on behalf of American Society for Bone and Mineral Research (ASBMR).

show abstract

Density of a sample bovine cortical bone matrix and its solid constituent in various media

Cited by 25 publications

References 10 publications

A mixed packing model for bone collagen

A mixed packing model for bone collagen

Diagenetic Alterations to Vertebrate Mineralized Tissues – A Critical Review

Subcanalicular Nanochannel Volume Is Inversely Correlated With Calcium Content in Human Cortical Bone

Contact Info

Product

Resources

About