Density Gradient Centrifugation for Enrichment and Identification of GFP-tagged Chitosomal Microvesicles of Filamentous Fungi

Verdín, Jorge; Sánchez‐León, Eddy; Fajardo-Somera, Rosa A.; Morales, Carlos A. Leal; Bartnicki-García, S.; Riquelme, Meritxell

doi:10.21769/bioprotoc.1611

Cited by 2 publications

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“…In addition, co-immunoprecipitation followed by LC/MS of CHS-1, CHS-4, and CHS-5 detected YPT-1 as one of the putative interacting proteins ( Fajardo-Somera et al, 2015 ). Furthermore, YPT-1 sedimented in fractions with a density range similar to the density of fractions with high CHS activity ( Bartnicki-Garcia et al, 1984 , Leal-Morales et al, 1994 , Verdín et al, 2009 , Verdín et al, 2015 ). Together, the evidence points to YPT-1 being the Rab involved in the traffic of chitosomes in N. crassa .…”

Section: Behind the Scenes To A Successful Destination: The Path Of Tmentioning

confidence: 90%

Off the wall: The rhyme and reason of Neurospora crassa hyphal morphogenesis

et al. 2019

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Highlights Chitin and β-1,3-glucan synthases are transported separately in chitosomes and macrovesicles. Chitin synthases occupy the core of the SPK; β-1,3-glucan synthases the outer layer. CHS-4 arrival to the SPK and septa is CSE-7 dependent. Rabs YPT-1 and YPT-31 localization at the SPK mimics that of chitosomes and macrovesicles. The exocyst acts as a tether between the SPK outer layer vesicles and the apical PM.

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Section: Behind the Scenes To A Successful Destination: The Path Of Tmentioning

confidence: 90%

Off the wall: The rhyme and reason of Neurospora crassa hyphal morphogenesis

et al. 2019

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“… Note: The Bio-Rad Model 2110 manual ( https://www.bio-rad.com/webroot/web/pdf/lsr/literature/M7318120.pdf ) is a great guide for connecting the column to the fraction collector. Additionally, references ( Behal et al., 2009 ; Verdin et al, 2015 ) provide images for the overall set up of the system. To fractionate the gradients, first insert a 1-mm glass capillary tube attached to 1 mm I.D.…”

Section: Before You Beginmentioning

confidence: 99%

Structural and biophysical characterization of the nucleosome-binding PZP domain

et al. 2021

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Summary The core subunit of the MORF acetyltransferase complex BRPF1 contains a unique combination of zinc fingers, including a plant homeodomain (PHD) finger followed by a zinc knuckle and another PHD finger, which together form a PZP domain (BRPF1 PZP ). BRPF1 PZP has been shown to bind to the nucleosome and make contacts with both histone H3 tail and DNA. Here, we describe biophysical and structural methods for characterization of the interactions between BRPF1 PZP , H3 tail, DNA, and the intact nucleosome. For complete details on the use and execution of this protocol, please refer to Klein et al. (2020) .

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Density Gradient Centrifugation for Enrichment and Identification of GFP-tagged Chitosomal Microvesicles of Filamentous Fungi

Cited by 2 publications

References 0 publications

Off the wall: The rhyme and reason of Neurospora crassa hyphal morphogenesis

Off the wall: The rhyme and reason of Neurospora crassa hyphal morphogenesis

Structural and biophysical characterization of the nucleosome-binding PZP domain

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