Density-Gradient and Chromatographic Fraction of Leptospiral Lipase

Physiology and evolution of spirochetes.

“…Other reports on the dissimilatory metabolism of leptospires describe studies on lipase activity (16,17,41,53,94,104,108,133,134), transamination reactions (122), and various enzymatic activities (35,63,65,66).…”

Partial Purification of an Extracellular Leptospiral Lipase

“…It has been observed that precipitation of the proteins from leptospiral culture fluids by large excesses of ethanol does not increase lipase specific activity (2,3,12). However, the precipitate resulting at 10% (v/v) ethanol concentrations was 14-fold higher in specific activity ( Table 1).…”

“…Leptospirae occur in the mammalian circulation where free fatty acid concentration is relatively low. The lipases (EC 3.1.1.3) secreted by leptospirae convert triglycerides to fatty acids (2,3,5,9,12). Concentrates of the enzyme have been prepared from culture media by repeated ethanol precipitation, dialysis, and lyophilization (12).…”