Densitometric thin-layer chromatographic determination of aescin in a herbal medicinal product containing Aesculus and Vitis dry extracts

Apers, Sandra; Naessens, Tania; Pieters, Luc; Vlietinck, A. J.

doi:10.1016/j.chroma.2005.10.069

Cited by 32 publications

(18 citation statements)

References 13 publications

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“…Information on physico-chemical and phytochemical parameters generated through the present study could be incorporated in standardization of quality, purity and sample identification of both varieties of P. heyneanus. Thin Layer Chromatography is a widely used technique for screening of herbal materials and herbal products and to differentiate between herbal species due to its simplicity, cost effectiveness, less time consuming and the ability of changing the solvent system [18,19]. Further, use of TLC fingerprint pattern for determining the identity, stability, and consistency of traditional medicine and identification of adulterants included to Panax species have been investigated [20,21].…”

Section: Resultsmentioning

confidence: 99%

Screening of Local and Introduced Varieties of <i>Pogostemon heyneanus</i> Benth. (Lamiaceae), for Superior Quality Physical, Chemical and Biological Parameters

Dharmadasa¹,

Rathnayake²,

Abeysinghe³

et al. 2014

WJAR

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Pogostemon heyneanus Benth. (Lamiaceae) is an aromatic, perfumery important, industrial crop widely cultivated in many Asian countries for its distinguished fragrance and other therapeutic purposes. However, commercial cultivation of P. heyneanus was hampered due to lack of high quality planting materials. Purpose of the present study is to explore superior quality P. heyneanus variety by means of physical (morphological), chemical (physico-chemical, phytochemical, essential oil content and composition) and biological [total antioxidant capacity (TAC)] parameters in order to establish commercial cultivation. Morphological, physico-chemical and phytochemical analysis were performed according to the methods described in WHO guidelines and other classical texts. The TAC was performed using Ferric Reducing Antioxidant Power (FRAP) assay. Essential oil was analyzed by gas chromatography Mass Spectrometry (GC/MS). Out of 26 morphological characters assessed, 5 characters were i.e. plant height, leaf margin, leaf apex, and leaf base and leaf shape polymorphic. All phytochemicals tested were identical to both varieties. However, presence of a prominent spots at Rf 0.12 (dark brown spot), 0.20 (rose colour spot), 0.45 (dark green spot) were characteristic for local variety. Significantly higher total ash content (12.32 %), oil content (0.52%), higher number of compounds in essential oil, patchouli alcohol content (57.0 %) and antioxidant capacity (108.53 ± 2.5 mg Trolox equivalent per g of extract) were reported in introduced variety. According to the results, introduced variety possesses superior quality physical, chemical and biological properties and therefore, introduced variety could be recommended for establishment of commercial cultivation.

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Section: Resultsmentioning

confidence: 99%

Screening of Local and Introduced Varieties of <i>Pogostemon heyneanus</i> Benth. (Lamiaceae), for Superior Quality Physical, Chemical and Biological Parameters

Dharmadasa¹,

Rathnayake²,

Abeysinghe³

et al. 2014

WJAR

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“…A voucher specimen is deposited at the Densitometric Determination. In recent years an analysis of the aescin content by densitometric thin-layer chromatography was developed [5,12,13]. The plant materials were air-dried at room temperature and ground to powder using a laboratory mill, then assayed for aescin content according to Costantini [5] using the TLC method.…”

mentioning

confidence: 99%

Aescin determination in seeds and capsules of three pure and hybrid Aesculus species

Gleńsk

Włodarczyk

Bassarello³

et al. 2011

Chem Nat Compd

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The genus Aesculus L. consists of 13 species, distributed in the northern hemisphere, primarily in eastern Asia and eastern North America, with one species native to Europe, one to western North America, and one to northwestern Mexico [1]. No previous phytochemical studies were carried out on the investigated species. According to earlier reports on other species, Aesculus hippocastanum contains an aescin that is a natural mixture of triterpene saponins. The aglycons are derivatives of protoaescygenin, acylated by acetic acid at C-22 and by either angelic or tiglic acids at C-21. Two forms exists, D and E, which can be distinguished by melting point, specific rotation, hemolytic index, and solubility in water. A number of other products have been isolated from chestnut seeds, i.e., flavonoids such as quercetin, as well as antioxidants such as proanthocyanidin A 2 and the coumarins esculin and fraxin [2][3][4][5][6]. However, all of these products can be found in larger amounts in other sources; furthermore, it was concluded that the antiedematous, antiexudative, and vasoprotective activities of hippocastanus extracts (HCE) are exclusively due to aescin [4]. From the ethanolic extract of the roots of Aesculus assamica two novel triterpenoid saponins with insulin-like activity were isolated [7]. Also identification of novel saponins from edible seeds of Japanese horse chestnut (Aesculus turbinata) after treatment with wooden ashes and their nutraceutical activity were recently reported [8]. It is worth noting that sapogenols from Aesculus hippocastanum showed significant in vitro cytotoxicity in the 9-KB cell culture assay [9].Aescin occurrence and quantitative determination in seeds and capsules of three Aesculus species was performed by TLC densitometry. Surprisingly the highest aescin content was found in Aesculus u marylandica capsules, not seeds, whereas there was a lack of this substance in Aesculus parviflora capsules. In case of Aesculus u hemiacantha, the seeds were a richer source than capsules (Table 1). Moreover, from capsules of Aesculus u marylandica two sapogenins were isolated: barringtogenol C and protoaescygenin. The latter two compounds are ubiquitious metabolites of the genus Aesculus; however, their presence in capsules of Aesculus u marylandica was not previously described. It is also worth noting that Aesculus u marylandica, especially Aesculus parviflora, is resistant to Cameraria ohridella -horse chestnut leafminer [10,11]. However, our findings indicate that the high aescin content most probably does not determine their resistance against the moth.Plant Material. Both mature seeds and capsules of Aesculus u hemiacantha Topa (hybrid of A. hippocastanum and A. turbinata) and Aesculus u marylandica Booth ex Kirchn. (hybrid of A. flava and A. glabra) were collected at the Botanical Garden of Wroclaw University in 2005. The mature seeds and capsules of Aesculus parviflora Walt. were collected at Wojslawice Arboretum in 2005. All plant material was dried in a dark place in an oven at 40qC. A vou...

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“…This can be carried out directly on the raw TLC plate or after derivatization with a chemical or biological reagent, for example, to estimate a biological activity. Over the past 15 years, the scanning of TLC plates employing optical instrumentation has been increasingly used, notably in the field of quality control of herbal drugs, where both fingerprint and quantitative assessment are required (Apers et al, 2006;Bhandari et al, 2007;Bhope et al, 2011). Herbal raw material or herbal drugs might be contaminated by toxins released into the environment, such as pesticides.…”

Section: Advances In Tlc For Plant Analysismentioning

confidence: 99%

Thin‐layer Chromatography of Plants, with Chemical and Biological Detection Methods

Urbain

Simões-Pires

2014

Encyclopedia of Analytical Chemistry

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Thin‐layer chromatography ( TLC ) is a widespread separation technique in the field of plant analysis and offers multiple advantages. This versatile technique can provide useful information about the qualitative or semiquantitative chemical composition of a mixture, thanks to coupling, with the detection and spectroscopic techniques and devices for image treatment. Moreover, it can contribute to the detection of biological activity by combining chromatography with bioassays, such as antimicrobial or enzyme inhibition tests. This procedure, providing simultaneous chromatographic separation and in situ assessment of a given biological activity, is named TLC bioautography. The major advantage of this technique is the ability to locate active substances from a complex mixture without prior sample purification. This avoids time‐consuming and tedious isolation of already‐known or inactive compounds. As this method is simple and cost effective, it is suitable for preliminary rapid screening to detect potential activity, as well as being a useful monitoring tool to track the bioguided isolation of active components. In this chapter, we focus on up‐to‐date TLC bioautographic assays, detail their mechanisms and applications in the field of natural drug discovery, and discuss the recent advances of this method that might appear at first glance to be a trivial approach.

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Densitometric thin-layer chromatographic determination of aescin in a herbal medicinal product containing Aesculus and Vitis dry extracts

Cited by 32 publications

References 13 publications

Screening of Local and Introduced Varieties of <i>Pogostemon heyneanus</i> Benth. (Lamiaceae), for Superior Quality Physical, Chemical and Biological Parameters

Screening of Local and Introduced Varieties of <i>Pogostemon heyneanus</i> Benth. (Lamiaceae), for Superior Quality Physical, Chemical and Biological Parameters

Aescin determination in seeds and capsules of three pure and hybrid Aesculus species

Thin‐layer Chromatography of Plants, with Chemical and Biological Detection Methods

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