Densitometric Determination of Hecogenin from Agave americana Leaf Using HPTLC

Ghoghari, Ashok; Rajani, M.

doi:10.1365/s10337-006-0826-6

Cited by 23 publications

(13 citation statements)

References 7 publications

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“…Derivatization of the analytes was done with anisaldehyde-sulfuric acid reagent followed by heating of the TLC plate for 3 min at 100 C. The yellow band corresponding to hecogenin (1) was densitometrically quantified at 430 nm. This simple, sensitive and accurate method can be routinely used for quality control of A. americana plant material (Ghoghari and Rajani, 2006). Similar research reported the simultaneous quantitation of …”

Section: Chemical Profilingsupporting

confidence: 57%

“…Source Reference(s) 1 (25R)-5a-Spirostan-3b-ol-12-one (Hecogenin) A. americana (leaves) Dewidar and El-Munajjed (1971); Ghoghari and Rajani (2006) A. americana (fermented leaves) A. intermixta (leaf juice) Quilez et al (2004) A. sisalana (leaves, juice) Chen et al (2011);Cripps and Blunden (1978); Sitton et al (1982); Tsung et al (1976b) A. wightii (submerged cultures) Sharma and Khanna (1980) A. wightii (leaves) Khanna et al (1979) A. aurea (leaves) Blunden et al (1980b) A. avellanidens (leaves) Blunden et al (1980b) A. cerulata (leaves) Blunden et al (1980b) A. cerulata ssp. subcerulata (leaves) Blunden et al (1980b) A. cocui (leaves) Blunden et al (1980b) (25R)-5a-Spirost-9(11)-en-3b-ol-12-one (9-Dehydrohecogenin)…”

Section: Namementioning

confidence: 99%

“…Conventionally, sapogenins and saponins of Agave were detected using 10e50% sulfuric acid followed by heating at 100 C (Blunden et al, 1980a;Sharma et al, 2012;Yi et al, 1989), Libermann-Burchard reagent (da Silva et al, 2006;Ding et al, 1993;Yu et al, 2011) (da Silva et al, 2002(da Silva et al, , 2005da Silva and Parente, 2007), respectively. Ghoghari and Rajani (2006) have developed an HPTLC method for the determination of hecogenin (1) in the leaf powder of Agave americana. The mobile phase used for the analysis was toluene/ ethyl acetate/methanol (6:1:0.8).…”

Section: Chemical Profilingmentioning

confidence: 99%

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Saponins of Agave: Chemistry and bioactivity

2016

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Section: Chemical Profilingsupporting

confidence: 57%

Section: Namementioning

confidence: 99%

Section: Chemical Profilingmentioning

confidence: 99%

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Saponins of Agave: Chemistry and bioactivity

2016

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“…The phytochemical evaluation of the ethanolic leaf extract of S. lehmbachii revealed the presence of alkaloids, tannins, saponins, terpenoides, flavonoids, steroids and resins which showed that the plant is of high pharmacological importance. These classes of compounds have been reported to show important biological effects (Longanga-Otshudi et al, 2000;Ghoghari and Rajan, 2006;Panda and Kar, 2007) and the presence of these constituents may be responsible for the anti-diarrhoeal properties seen in the extract. Earlier studies have shown that antidiarhoeal potential of medicinal plants were due to its secondary metabolites (Kouitcheu et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

Studies on gastrointestinal properties of ethanolic leaf extract of Salacia lehmbachii in Wistar rats

Essien¹,

Grace²,

Akuodor³

et al. 2016

Afr. J. Pharm. Pharmacol.

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Salacia lehmbachii Loes, is used traditionally in Nigeria for the treatment of gastrointestinal disorders. The aim of this study was to investigate the anti-ulcer and anti-diarrhoeal activities of the ethanolic leaf extract of S. lehmbachii. The ethanolic leaf extract was evaluated for castor oil-induced diarrhoeal, intestinal transit as well as intestinal fluid accumulation in rats, while indomethacin, water immersion stress-induced and histamine were used for anti-ulcer tests. The extract at the doses used significantly (P<0.05) decreased castor oil-induced diarrhoea in rats as judged by a decrease in the number of wet faeces in the extract treated rats. Moreso, the leaf extract inhibited the propulsive movement of intestinal contents. S. lehmbachii also showed a dose-related inhibitory activity on castor oil-induced intestinal fluid accumulation in rats. The leaf extract of S. lehmbachii significantly (P<0.05) reduced the ulcer index in all assays used. The results of the current study support the folkloric usage of S. lehmbachii leaf extract in the management of gastrointestinal disorders in Nigerian herbal traditional medicine.

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“…9 In addition, hecogenin is an important precursor for the synthesis of steroidal hormones. 10 Moreover, hecogenin has a wide spectrum of pharmacological activities already reported. 11−13 As a function of the antiproliferative activity of hecogenin, the most studied effects of this sapogenin are those related to cancer research.…”

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confidence: 99%

Evidence for the Involvement of Descending Pain-Inhibitory Mechanisms in the Antinociceptive Effect of Hecogenin Acetate

et al. 2013

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Hecogenin is a sapogenin present in the leaves of species from the Agave genus, with a wide spectrum of reported pharmacological activities. The present study was undertaken to evaluate whether hecogenin acetate (1) has antinociceptive properties and to determine its mechanism of action. The nociceptive threshold was evaluated using the tail flick test in mice. Mice motor performance was evaluated in a Rotarod test. By using Fos expression as a marker of neural activation, the involvement of the periaqueductal gray in 1-induced antinociception was evaluated. Intraperitoneal administration of 1 (5-40 mg/kg) produced a dose-dependent increase in the tail flick latency time compared to vehicle-treated group (p < 0.01). Mice treated with 1 (40 mg/kg) did not show motor performance alterations. The antinociception of 1 (40 mg/kg) was prevented by naloxone (nonselective opioid receptor antagonist; 5 mg/kg), CTOP (μ-opioid receptor antagonist; 1 mg/kg), nor-BNI (κ-opioid receptor antagonist; 0.5 mg/kg), naltrindole (δ-opioid receptor antagonist; 3 mg/kg), or glibenclamide (ATP-sensitive K(+) channel blocker; 2 mg/kg). Systemic administration of 1 (5-40 mg/kg) increased the number of Fos positive cells in the periaqueductal gray. The present study has demonstrated for the first time that 1 produces consistent antinociception mediated by opioid receptors and endogenous analgesic mechanisms.

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Densitometric Determination of Hecogenin from Agave americana Leaf Using HPTLC

Cited by 23 publications

References 7 publications

Saponins of Agave: Chemistry and bioactivity

Saponins of Agave: Chemistry and bioactivity

Studies on gastrointestinal properties of ethanolic leaf extract of Salacia lehmbachii in Wistar rats

Evidence for the Involvement of Descending Pain-Inhibitory Mechanisms in the Antinociceptive Effect of Hecogenin Acetate

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