Denizli Bölgesinde Kestane (Castanea sativa) Ağacından Cryptococcus neoformans İzolasyonu

Şengül, Mustafa; Kutlu, Murat; Döğen, Aylin; Aksoy, Levent; Gonca, Serpil; İlkit, Macit; Ergin, Çağrı

doi:10.5578/mb.67583

Cited by 2 publications

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“…gattii isolation from trees in Australia (Ellis and Pfeiffer, 1990), many studies have confirmed the environmental association of Cryptococcus with plants in different climatic zones (Granados and Castañeda, 2005, 2006; Randhawa et al, 2008, 2011; Bedi et al, 2012; Chowdhary et al, 2012). Several studies have characterized the properties of these yeasts that contribute to the colonization of new environmental niches (Granados and Castañeda, 2006; Randhawa et al, 2008; Ergin and Kaleli, 2010; Ergin et al, 2014; Sengul et al, 2019). With the exception of iatrogenic (Baddley et al, 2011) and zoonotic (Nosanchuk et al, 2000; Lagrou et al, 2005; Singh et al, 2018) cases, Cryptococcus infection is caused by the inhalation of airborne basidiospores or desiccated yeast cells from the environment (Hull et al, 2005; Lin and Heitman, 2006; Velagapudi et al, 2009; Springer et al, 2013; May et al, 2016), emphasizing the importance of identifying the natural reservoirs of C .…”

Section: Introductionmentioning

confidence: 99%

“…neoformans in the Mediterranean region (Mahmoud, 1999; Bernardo et al, 2001; Campisi et al, 2003; Ergin et al, 2004; Mseddi et al, 2011; Romeo et al, 2011, 2012; Colom et al, 2012; Gokçen and Ergin, 2014; Cogliati et al, 2016a,b; Elhariri et al, 2016; Ellabib et al, 2016). Cryptococcus neoformans tree colonization has been observed in northern Mediterranean countries such as Spain (Colom et al, 2012; Cogliati et al, 2016a), Portugal (Bernardo et al, 2001; Ferreira et al, 2014; Cogliati et al, 2016a), France (Cogliati et al, 2016a,b), Italy (Campisi et al, 2003; Romeo et al, 2011, 2012; Cogliati et al, 2016a,b), Greece (Cogliati et al, 2016a,b), and Turkey (Ergin et al, 2004; Ergin, 2010; Ergin and Kaleli, 2010; Gokçen and Ergin, 2014; Cogliati et al, 2016a,b; Sengul et al, 2019), as well as in the northern parts of Cyprus (Cogliati et al, 2016a), Libya (Cogliati et al, 2016a; Ellabib et al, 2016), Tunisia (Mseddi et al, 2011), and Egypt (Mahmoud, 1999; Elhariri et al, 2016). The olive tree is one of the oldest known cultivated trees in the world and is grown in the entire Mediterranean basin mostly for commercial reasons (Uylaşer and Yildiz, 2014).…”

Section: Introductionmentioning

confidence: 99%

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Cryptococcus neoformans Recovered From Olive Trees (Olea europaea) in Turkey Reveal Allopatry With African and South American Lineages

Ergin

Şengül

Aksoy

et al. 2019

Front. Cell. Infect. Microbiol.

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Cryptococcus species are life-threatening human fungal pathogens that cause cryptococcal meningoencephalitis in both immunocompromised and healthy hosts. The natural environmental niches of Cryptococcus include pigeon (Columba livia) guano, soil, and a variety of tree species such as Eucalyptus camaldulensis, Ceratonia siliqua, Platanus orientalis, and Pinus spp. Genetic and genomic studies of extensive sample collections have provided insights into the population distribution and composition of different Cryptococcus species in geographic regions around the world. However, few such studies examined Cryptococcus in Turkey. We sampled 388 Olea europaea (olive) and 132 E. camaldulensis trees from seven locations in coastal and inland areas of the Aegean region of Anatolian Turkey in September 2016 to investigate the distribution and genetic diversity present in the natural Cryptococcus population. We isolated 84 Cryptococcus neoformans strains (83 MATα and 1 MATa) and 3 Cryptococcus deneoformans strains (all MATα) from 87 (22.4% of surveyed) O. europaea trees; a total of 32 C. neoformans strains were isolated from 32 (24.2%) of the E. camaldulensis trees, all of which were MATα. A statistically significant difference was observed in the frequency of C. neoformans isolation between coastal and inland areas (P < 0.05). Interestingly, the MATa C. neoformans isolate was fertile in laboratory crosses with VNI and VNB MATα tester strains and produced robust hyphae, basidia, and basidiospores, thus suggesting potential sexual reproduction in the natural population. Sequencing analyses of the URA5 gene identified at least five different genotypes among the isolates. Population genetics and genomic analyses revealed that most of the isolates in Turkey belong to the VNBII lineage of C. neoformans, which is predominantly found in southern Africa; these isolates are part of a distinct minor clade within VNBII that includes several isolates from Zambia and Brazil. Our study provides insights into the geographic distribution of different C. neoformans lineages in the Mediterranean region and highlights the need for wider geographic sampling to gain a better understanding of the natural habitats, migration, epidemiology, and evolution of this important human fungal pathogen.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Cryptococcus neoformans Recovered From Olive Trees (Olea europaea) in Turkey Reveal Allopatry With African and South American Lineages

Ergin

Şengül

Aksoy

et al. 2019

Front. Cell. Infect. Microbiol.

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show abstract

“…Following the first report of C. neoformans isolation from trees in Australia (Ellis and Pfeiffer, 1990), many studies have confirmed the environmental association of Cryptococcus with plants in different climatic zones (Granados andCastañeda, 2005, 2006;Randhawa et al, 2008Randhawa et al, , 2011Bedi et al, 2012;Chowdhary et al, 2012). Several studies have characterized the properties of these yeasts that contribute to the colonization of new environmental niches (Granados and Castañeda, 2006;Randhawa et al, 2008;Ergin and Kaleli, 2010;Ergin et al, 2014;Sengul et al, 2019). With the exception of iatrogenic (Baddley et al, 2011) and zoonotic (Nosanchuk et al, 2000;Lagrou et al, 2005;Singh et al 2018) cases, Cryptococcus infection is caused by the inhalation of airborne basidiospores or desiccated yeast cells from the environment (Hull et al, 2005;Lin and Heitman, 2006;Velagapudi et al, 2009;Springer et al, 2013;May et al, 2016), emphasizing the importance of identifying the natural reservoirs of C. neoformans and the molecular links between environmental and clinical isolates and their association with disease (Litvintseva et al, 2005;Noguera et al, 2015;Chen et al, 2015;Kangogo et al, 2015;Spina-Tensini et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Cryptococcus neoformansrecovered from olive trees (Olea europaea) in Turkey reveal allopatry with African and South American lineages

Ergin

Şengül

Aksoy

et al. 2019

Preprint

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Cryptococcus species are life-threatening human fungal pathogens that cause cryptococcal meningoencephalitis in both immunocompromised and healthy hosts. The natural environmental niches of Cryptococcus include pigeon (Columba livia) guano, soil, and a variety of tree species such as Eucalyptus camaldulensis, Ceratonia siliqua, Platanus orientalis, and Pinus spp. Genetic and genomic studies of extensive sample collections have provided insights into the population distribution and composition of different Cryptococcus species in geographic regions around the world. However, few such studies examined Cryptococcus in Turkey. We sampled 388 Olea europaea (olive) and 132 E. camaldulensis trees from 7 locations in coastal and inland areas of the Aegean region of Anatolian Turkey in September 2016 to investigate the distribution and genetic diversity present in the natural Cryptococcus population. We isolated 84 Cryptococcus neoformans strains (83 MATα and 1 MATa) and 3 Cryptococcus deneoformans strains (all MATα) from 87 (22.4% of surveyed) O. europaea trees; a total of 32 C. neoformans strains were isolated from 32 (24.2%) of the E. camaldulensis trees, all of which were MATα. A statistically significant difference was observed in the frequency of C. neoformans isolation between coastal and inland areas (P < 0.05). Thus, O. europaea trees could represent a novel niche for C. neoformans. Interestingly,the MATa C. neoformans isolate was fertile in laboratory crosses with VNI and VNB MATα tester strains and produced robust hyphae, basidia, and basidiospores, thus suggesting potential sexual reproduction in the natural population. Sequencing analyses of the URA5 gene identified at least 5 different genotypes among the isolates. Population genetics and genomic analyses revealed that most of the isolates in Turkey belong to the VNBII lineage of C. neoformans, which is predominantly found in southern Africa; these isolates are part of a distinct minor clade within VNBII that includes several isolates from Zambia and Brazil. Our study provides insights into the geographic distribution of different C. neoformans lineages in the Mediterranean region and highlights the need for wider geographic sampling to gain a better understanding of the natural habitats, migration, epidemiology, and evolution of this important human fungal pathogen.

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Denizli Bölgesinde Kestane (Castanea sativa) Ağacından Cryptococcus neoformans İzolasyonu

Cited by 2 publications

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Cryptococcus neoformans Recovered From Olive Trees (Olea europaea) in Turkey Reveal Allopatry With African and South American Lineages

Cryptococcus neoformans Recovered From Olive Trees (Olea europaea) in Turkey Reveal Allopatry With African and South American Lineages

Cryptococcus neoformansrecovered from olive trees (Olea europaea) in Turkey reveal allopatry with African and South American lineages

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