Denitrifying sulfide removal process on high-salinity wastewaters in the presence of Halomonas sp.

Liu, Chunshuang; Zhao, Dixian; Ma, Wenjie; Yao, Guowei; Wang, Aijie; Wang, Qilin; Lee, Dong Hoon

doi:10.1007/s00253-015-7039-6

Cited by 223 publications

(108 citation statements)

References 25 publications

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“…In a continuous integrated autotrophic-heterotrophic denitrification bioreactor, H. desiderata was observed to dominate, showing the ability to simultaneously remove sulfide and nitrate (Zhang et al, 2020). Halomonas was also found to dominate; it could enhance sulfide denitrification removal on high-salinity wastewaters (Liu et al, 2016a). Besides, bacterial thiosulfate oxidation was also found in Halomonas isolates from deep sea sediment (Teske et al, 2000;Du et al, 2019).…”

Section: Widespread Presence Of Sulfur Oxidation Pathways In the Wholmentioning

confidence: 98%

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Aerobic Denitrification and Heterotrophic Sulfur Oxidation in the Genus Halomonas Revealed by Six Novel Species Characterizations and Genome-Based Analysis

Wang

Shao

2021

Front. Microbiol.

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Bacteria of Halomonas are widely distributed in various environments and play a substantial role in the nutrient cycle. In this report, 14 strains capable of aerobic denitrification and heterotrophic sulfur oxidation were isolated from different habitats. Based on the phenotypic, genotypic, and chemotaxonomic analyses, these strains were considered to represent six novel species of the genus Halomonas, for which the names Halomonas zhangzhouensis sp. nov. type strain CXT3-11T ( = MCCC 1A11036T = KCTC 72087T), Halomonas aerodenitrificans sp. nov. CYD-9T ( = MCCC 1A11058T = KCTC 72088T), Halomonas sulfidoxydans sp. nov. CYN-1-2T ( = MCCC 1A11059T = KCTC 72089T), Halomonas ethanolica sp. nov. CYT3-1-1T ( = MCCC 1A11081T = KCTC 72090T), Halomonas sulfidivorans sp. nov. NLG_F1ET ( = MCCC 1A13718T = KCTC 72091T), and Halomonas tianxiuensis sp. nov. BC-M4-5T ( = MCCC 1A14433T = KCTC 72092T) are proposed. Intriguingly, they formed a unique group with 11 other species designated as the “H. desiderata group.” To better understand their featured metabolisms, genes involved in denitrification and sulfur oxidation were analyzed, along with 193 other available genomes of the whole genus. Consistently, complete denitrification pathways were confirmed in the “H. desiderata group,” in which napA, narG, nirS, norB, and nosZ genes coexist. Their nitrite reductase NirS formed a unique evolutionary lineage, distinguished from other denitrifiers in Halomonas. In addition, diverse occurrence patterns of denitrification genes were also observed in different phylogenetic clades of Halomonas. With respect to sulfur oxidation, fccAB genes involved in sulfide oxidation commonly exist in the “H. desiderata group,” while sqr genes are diverse and can be found in more species; sqr genes co-occurred with fccAB in eight strains of this study, contributing to more active sulfide oxidation. Besides, the tsdA gene, which encodes an enzyme that oxidizes thiosulfate to tetrathionate, is ubiquitous in the genus Halomonas. The widespread presence of sqr/fccAB, pdo, and tsdA in Halomonas suggests that many Halomonas spp. can act as heterotrophic sulfur oxidizers. These results provide comprehensive insights into the potential of denitrification and sulfur oxidation in the whole genus of Halomonas. With regard to the global distribution of Halomonas, this report implies their unneglectable role in the biogeochemical cycle.

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Section: Widespread Presence Of Sulfur Oxidation Pathways In the Wholmentioning

confidence: 98%

“…Several studies have shown that Halomonas spp. dominate in bioreactors and play important roles in the removal of sulfide and nitrate (Liu et al, 2016a;Zhang et al, 2020). However, their sulfur oxidation mechanisms are unclear.…”

Section: Introductionmentioning

confidence: 99%

Aerobic Denitrification and Heterotrophic Sulfur Oxidation in the Genus Halomonas Revealed by Six Novel Species Characterizations and Genome-Based Analysis

Wang

Shao

2021

Front. Microbiol.

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“…As one of the most abundant genera in the ocean, Halomonas bacteria were described as opportunitrophic microbes with broad functional potential, and most denitrifying bacteria within this genus belonged to NirS-type denitrifiers. The widespread distribution in various saline environments and satisfying contribution to nitrogen removal in bioreactors of Halomonas [24,25] suggest that this genus plays an important ecological role in nitrogen cycling in some saline or even highsalt environments. As a member of NirS-type denitrifiers in Halomonas, strain HN2 may have an adaptive advantage in various environments supporting denitrification activity and increased potential in nitrogen removal from saline wastewater.…”

Section: Discussionmentioning

confidence: 99%

“…The nitrogen removal abilities, especially denitrification of strains from genus Halomonas have been reported in previous works, such as H. campisalis ha3 [11] and Halomonas cerina 15CR [23]. Several studies have shown that Halomonas was dominant in bioreactors and contributed to satisfactory nitrogen and carbon removal efficiencies [24,25]. However, the denitrification ability of Halomonas piezotolerans has not been reported.…”

Section: Introductionmentioning

confidence: 92%

Characteristics and mechanism of heterotrophic nitrification/aerobic denitrification in a novel Halomonas piezotolerans strain

Dong

et al. 2021

J Basic Microbiol

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A strain was isolated from an activated sludge system and identified as Halomonas piezotolerans HN2 in this study, which is the first strain in H. piezotolerans with the capability of heterotrophic nitrification and aerobic denitrification. Strain HN2 showed the maximum nitrogen removal rate of 9.10 mg/L/h by utilizing ammonium at the salinity of 3.0%. Under saline environment, HN2 could remove nitrogen efficiently in neutral and slightly alkaline environments, with the carbon sources of sodium succinate and sodium citrate and the C/N ratio of 15-20, and the maximum removal efficiencies of ammonium, nitrite, and nitrate were 100%, 96.35%, and 99.7%, respectively.The genomic information revealed the presence of amoA, napA, and nosZ genes in strain HN2, and the target bands of nirS were obtained via a polymerase chain reaction. Therefore, we inferred that ammonium was mainly utilized for the growth of strain HN2 through assimilation, and another part of the initial ammonium was converted into nitrate through nitrification, and then into gaseous nitrogen through denitrification. This report indicated the potential application of strain HN2 and other nitrifying and denitrifying Halomonas strains in the removal of nitrogen pollution in marine-related environments and also implies the important role of Halomonas in the nitrogen cycle process of the ocean.

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“…Both of these genera have been previously reported as dominant members of the rotifer microbiota 14,54,55 , and this genus is likely transferred to the water through addition of live feed. Species within Kangiella and Arcobacter both metabolize nitrogen compounds 56–58 and may play a role in nitrogen cycling in aquaculture. An increase in these genera in probiotic-treated systems may indicate more efficient removal of nitrogenous fish waste from the system.…”

Section: Discussionmentioning

confidence: 99%

Benefits of a Bacillus probiotic to larval fish survival and transport stress resistance

Tarnecki

Wafapoor

Phillips

et al. 2019

Sci Rep

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The need for sustainable bacterial management approaches in aquaculture is crucial for advancement of the industry. Probiotics are a promising strategy as evidenced by benefits demonstrated in intensive larviculture of various marine fish species. In this study we investigate the effects of a mixed Bacillus species (B. licheniformis and B. amyloliquefaciens) probiotic on rearing of larval common snook (Centropomus undecimalis). Experimental treatments included (1) probiotics supplemented to the water and live feed, (2) probiotics supplemented to the water only, and (3) no probiotic controls. Data from two separate trials indicated up to 2.5 times higher survival with probiotic addition, as well as 20% higher survival 7 days following a transport event. These benefits were not explained by faster growth, measured water quality parameters, or innate immune enzyme activities. Microbiota analysis indicated the importance of system stabilization prior to larval stocking to improve rearing success and probiotic performance. ied Potential probiotic benefits include accelerated gastrointestinal tract development, enhanced immunity, inhibition of opportunistic bacteria, and improvements to water quality parameters. Results suggest this probiotic should be tested in other marine fish species in order to reduce larval rearing bottlenecks.

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Denitrifying sulfide removal process on high-salinity wastewaters in the presence of Halomonas sp.

Cited by 223 publications

References 25 publications

Aerobic Denitrification and Heterotrophic Sulfur Oxidation in the Genus Halomonas Revealed by Six Novel Species Characterizations and Genome-Based Analysis

Aerobic Denitrification and Heterotrophic Sulfur Oxidation in the Genus Halomonas Revealed by Six Novel Species Characterizations and Genome-Based Analysis

Characteristics and mechanism of heterotrophic nitrification/aerobic denitrification in a novel Halomonas piezotolerans strain

Benefits of a Bacillus probiotic to larval fish survival and transport stress resistance

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