Denitrification with polycaprolactone as solid substrate in a laboratory-scale recirculated aquaculture system

Boley, A.; Müller, W.

doi:10.2166/wst.2005.0728

Cited by 49 publications

(18 citation statements)

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“…To date, SPD processes using poly(3-hydroxybutyrate) (PHB) 31) and its copolymer, poly(3-hydroxybutyrate-co-hydroxyvalarate) (PHBV) 6,26,30) , have been most intensively studied. The application of poly(ε-caprolactone) (PCL) to SPD processes has also been reported [4][5][6]22) .…”

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confidence: 99%

Activity and Community Composition of Denitrifying Bacteria in Poly(3-hydroxybutyrate-co-3-hydroxyvalerate)-Using Solid-phase Denitrification Processes

et al. 2007

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Two laboratory-scale solid-phase denitrification (SPD) reactors, designated reactors A and B, for nitrogen removal were constructed by acclimating activated sludge with pellets and flakes of poly(3-hydoxybutyrate-co-3-hydroxyvalerate) (PHBV) as the sole added substrate under denitrifying conditions, respectively. The average denitrification rate in both reactors was 60 mg NO3 − -N g −1 (dry wt) h −1 under steady-state conditions, whereas washed sludge taken from the reactors showed an average denitrification rate of 20 mg NO3-N g −1 (dry wt) hwith fresh PHBV as the sole substrate. The difference in the denitrification rate between the two might be due to the bioavailability of intermediate metabolites as the substrate for denitrification, because acetate and 3-hydroxybutyrate were detected in the reactors. Most of the predominant denitrifiers isolated quantitatively by the platecounting method using non-selective agar medium were unable to degrade PHBV and were identified as members of genera of the class Betaproteobacteria by studying 16S rRNA gene sequence information. nirS and nosZ gene clone library-based analyses of the microbial community from SPD reactor A showed that most of the nirS and nosZ clones proved to be derived from members of the family Comamonadaceae and other phylogenetic groups of the Betaproteobacteria. These results suggest that the efficiency of denitrification in the PHBV-SPD process is affected by the availability of intermediate metabolites as possible reducing-power sources as well as of the solid substrate, and that particular species of the Betaproteobacteria play the primary role in denitrification in this process.

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Activity and Community Composition of Denitrifying Bacteria in Poly(3-hydroxybutyrate-co-3-hydroxyvalerate)-Using Solid-phase Denitrification Processes

et al. 2007

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“…External solid substrates widely used for the SPD process are biodegradable polyesers applied as biodegradable plastics, such as poly(3-hydroxybutyrate) (PHB) [3] and its copolymer with 3-hydroxyvalerate (PHBV) [4][5][6]. Other biodegradable plastics used for denitrification are poly(ε-caprolactone) (PCL) [7][8][9][10][11], poly(butylene succinate) (PBS) [12], and poly(L-lactic acid) (PLLA) [13][14][15][16]. Nevertheless, the SPD process is yet to be applied for practical use as a plant-scale system.…”

Section: Introductionmentioning

confidence: 99%

Nitrate removal performance of Diaphorobacter nitroreducens using biodegradable plastics as the source of reducing power

Khan

Nagao

Hiraishi

2015

AIP Conference Proceedings

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Abstract. Strain NA10B T and other two strains of the denitrifying betaproteobacterium Diaphorobacter nitroreducens were studied for the performance of solid-phase denitrification (SPD) using poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) and some other biodegradable plastics as the source of reducing power in wastewater treatment. Sequencing-batch SPD reactors with these organisms and PHBV granules or flakes as the substrate exhibited good nitrate removal performance. Vial tests using cultures from these parent reactors showed higher nitrate removal rates with PHBV granules (ca. 20 mg-NO 3 --N g -1 [dry wt cells] h -1 ) than with PHBV pellets and flakes. In continuous-flow SPD reactors using strain NA10B T and PHBV flakes, nitrate was not detected even at a loading rate of 21 mg-NO 3 --N L -1 h -1 . This corresponded to a nitrate removal rate of 47 mg-NO 3 --N g -1 (dry wt cells) h -1 . In the continuous-flow reactor, the transcription level of the phaZ gene, coding for PHB depolymerase, decreased with time, while that of the nosZ gene, involved in denitrificaiton, was relatively constant. These results suggest that the bioavailability of soluble metabolites as electron donor and carbon sources increases with time in the continuous-flow SPD process, thereby having much higher nitrate removal rates than the process with fresh PHBV as the substrate.

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“…To avoid these problems, a new type of denitrification method has been designed in recent years, using insoluble biodegradable polymers as carbon source and biofilm carrier simultaneously, which is accessible only by enzymatic attack (Boley et al 2000). There are two kinds of solid carbon sources, namely natural materials, including wheat straw (Aslan and Türkman 2005;Fan et al 2012;Soares and Abeliovich 1998), cotton (Volokita et al 1996a), waste newspaper (Volokita et al 1996b), pine bark (Trois et al 2010a, b), crab-shell chitin (Robinson-Lora and Brennan 2009) and synthetic polymers, such as polyhydroxyalkanoates (PHAs) (Hiraishi and Khan 2003), polycaprolactone (PCL) (Boley et al 2000;Boley and Müller 2005;Wang and Wang 2009;Zhou et al 2009;Chu and Wang 2011a, b;Shen and Wang 2011;Chu and Wang 2013;Shen et al 2013a;Wu et al 2013a), PBS (Wu et al 2013b) and polylactic acid (PLA) Shen et al 2013b). However, synthetic polymers are expensive, while natural materials were much cheaper but may bring ammonia (Robinson-Lora and Brennan 2009), high dissolved organic carbon (DOC) release and color problems (Aslan and Türkman 2003).…”

Section: Introductionmentioning

confidence: 99%

Comparison of polycaprolactone and starch/polycaprolactone blends as carbon source for biological denitrification

Shen

Wang

et al. 2014

Int. J. Environ. Sci. Technol.

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The cross-linked starch/polycaprolactone (SPCL10) and starch/polycaprolactone (SPCL12) blends were prepared, characterized and used as carbon source and biofilm support for biological nitrate removal. The results showed that SPCL10 and SPCL12 had similar performance on water absorption (about 21 %) and leaching capacity. FTIR spectra confirmed the cross-linking reaction between starch and PCL. SEM displayed a thermoplastic nature of SPCL10 and SPCL12. These blends could serve as solid carbon source and biofilm support for biological denitrification, and the acclimation time of microbial biofilm on the surfaces of SPCL10, SPCL12 and PCL were about 2 days, 2 days and 16 days, respectively. The average denitrification rates were 0.0216, 0.0154 and 0.0071 mg NO 3 -N/(g h) for SPCL10, SPCL12 and PCL, respectively, and the effluent NO 2 -N concentration was below 1 mg/L at all cases. The phenomenon of ammonia formation was observed, but ammonia concentration was below 0.5 mg/L.

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Denitrification with polycaprolactone as solid substrate in a laboratory-scale recirculated aquaculture system

Cited by 49 publications

References 0 publications

Activity and Community Composition of Denitrifying Bacteria in Poly(3-hydroxybutyrate-co-3-hydroxyvalerate)-Using Solid-phase Denitrification Processes

Activity and Community Composition of Denitrifying Bacteria in Poly(3-hydroxybutyrate-co-3-hydroxyvalerate)-Using Solid-phase Denitrification Processes

Nitrate removal performance of Diaphorobacter nitroreducens using biodegradable plastics as the source of reducing power

Comparison of polycaprolactone and starch/polycaprolactone blends as carbon source for biological denitrification

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