Denitrification performance and microbial diversity in a packed-bed bioreactor using PCL as carbon source and biofilm carrier

Abstract

Polycaprolactone (PCL) was used as both carbon source and biofilm support for denitrifying bacteria in a packed-bed bioreactor. The denitrification performance and microbial diversity were investigated. The microbial community of biofilm developed on the surface of PCL in the reactor was analyzed by pyrosequencing method. The experimental results showed the average nitrate removal efficiency reached 93 % at stable operation. ESEM observation and FTIR analysis were conducted to characterize the PCL structure before and after microbial utilization. For the microbial community, Betaproteobacteria predominated, and most of the PCL-degrading denitrifying bacteria assigned to the family of Comamonadacea. Denitrifying bacteria accounted for more than 20 % in the total population, indicating that PCL is a good carrier and carbon source for biological denitrification.

Influence of hydraulic regimes and Cl2/NH3-N mass ratios on the bacterial structure and composition in an experimental flow cell chloraminated drinking water system

“…Other abundant genera species in this study, such as Gemmata and Porphyrobacter have been observed in drinking water samples and suggested to be adapted to oligotrophic conditions in DWDSs [58][59][60] . In addition, Azospira, which belongs to denitrifying genus 61 , was detected to be abundance as well. Combine with the discussion above, the decrease of nitrate nitrogen in this study could due to the possible occurrence of denitrification process.…”

Activated Sludge Microbial Community and Treatment Performance of Wastewater Treatment Plants in Industrial and Municipal Zones

“…Overall, Z-WWTP had higher abundances of Dechloromonas, Nitrospira, and Thauera, while Y-WWTP had higher Nitrosomonas concentrations and lower Nitrospira abundances. Dechloromonas and Thauera were identified as typical denitrifying bacteria in wastewater treatment [30], while Nitrosomonas and Nitrospira were predominant ammonia oxidation bacteria and nitrite oxidation bacteria in WWTPs [31][32][33]. In an SBR process, the Nitrospira population decreased sharply and was ultimately wiped out in the presence of copper [34].…”

Effects of Hydraulic Retention Time and Influent Nitrate-N Concentration on Nitrogen Removal and the Microbial Community of an Aerobic Denitrification Reactor Treating Recirculating Marine Aquaculture System Effluent

“…Desulfobacter and Desulfopila were detected in a constructed wetland to which starch/polylactic acid blends were added to support denitrification [103]. Denitratisoma was found capable of complete denitrification of wastewater [104,105]. We observed both Halomonas and Denitratisoma in our reactor, whose frequencies decreased with increasing influent nitrate-N concentration.…”