Denitrification gene pools, transcription and kinetics of  NO, N2O and N2 production as affected by soil pH

Liu, Binbin; Mørkved, Pål Tore; Frostegård, Åsa; Bakken, Lars R.

doi:10.1111/j.1574-6941.2010.00856.x

Cited by 419 publications

(386 citation statements)

References 35 publications

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“…Numerous studies have examined the factors that influence different pathways involved in denitrification, such as nitrate (Magalhães et al 2005), carbon availability (Henderson et al 2010), pH (Liu et al 2010), and copper concentration (Magalhães et al 2011;Felgate et al 2012). Nevertheless, compaction, as a physical factor, has not received as much attention as the above chemical factors associated with denitrification.…”

Section: Introductionmentioning

confidence: 99%

Compaction stimulates denitrification in an urban park soil using 15N tracing technique

Deng

Rensing

et al. 2013

Environ Sci Pollut Res

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Soils in urban areas are subjected to compaction with accelerating urbanization. The effects of anthropogenic compaction on urban soil denitrification are largely unknown. We conducted a study on an urban park soil to investigate how compaction impacts denitrification. By using 15 N labeling method and acetylene inhibition technique, we performed three coherent incubation experiments to quantify denitrification in compacted soil under both aerobic and anaerobic conditions. Uncompacted soil was set as the control treatment. When monitoring soil incubation without extra substrate, higher nitrous oxide (N 2 O) flux and denitrification enzyme activity were observed in the compacted soil than in the uncompacted soil. In aerobic incubation with the addition of K 15 NO 3 , N 2 O production in the compacted soil reached 10.11 ng N h −1 g −1 as compared to 0.02 ng N h −1 g −1 in the uncompacted soil. Denitrification contributed 96 % of the emitted N 2 O in the compacted soil and 36 % of the emitted N 2 O in the uncompacted soil; total denitrification rate was higher in the compacted soil (up to 79.35 ng N h −1 g −1 ) than in the uncompacted soil (0.11 ng N h −1 g −1 ). Under anaerobic incubation with the addition of K 15 NO 3 , no statistical difference in total N losses and 15 N-(N 2 O+N 2 ) flux between the uncompacted soil and the compacted soil was detected. Compaction promoted soil denitrification and may impact urban N biogeochemical cycling.

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Section: Introductionmentioning

confidence: 99%

Compaction stimulates denitrification in an urban park soil using 15N tracing technique

Deng

Rensing

et al. 2013

Environ Sci Pollut Res

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“…Studies have shown that denitrifying communities are affected by soil conditions including temperature, moisture, pH and substrate (e.g. NO 3 À , organic carbon) concentration (Braker et al, 2010;Henderson et al, 2010;Liu et al, 2010;Stres et al, 2008;Lalisse-Grundmann et al, 1988;Renner and Becker, 1970). High exposures to metals such as cadmium, copper, zinc and silver can also be detrimental to denitrifying communities (Holtan-Hartwig et al, 2002;McKenney and Vriesacker, 1985;Throbäck et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Effect of pyrene on denitrification activity and abundance and composition of denitrifying community in an agricultural soil

Guo

Deng

Qiao

et al. 2011

Environmental Pollution

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a b s t r a c tToxicity of pyrene on the denitrifiers was studied by spiking an agricultural soil with pyrene to a series of concentrations (0e500 mg kg À1 ) followed by doseeresponse and dynamic incubation experiments. Results showed a positive correlation between potential denitrification activity and copy numbers of denitrifying functional genes (nirK, nirS and nosZ), and were both negatively correlated with pyrene concentrations. Based on the comparison of EC 50 values, denitrifiers harboring nirK, nirS or nosZ gene were more sensitive than denitrification activity, and denitrifiers harboring nirS gene were more sensitive than that harboring nirK or nosZ genes. Seven days after spiking with EC 50 concentration of pyrene, denitrifiers diversity decreased and community composition changed in comparison with the control. Phylogenetic analyses of three genes showed that the addition of pyrene increased the proportion of Bradyrhizobiaceae, Rhodospirillales, Burkholderiales and Pseudomonadales. Some species belonging to these groups were reported to be able to degrade PAHs.

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“…Using 15 N-tracing experiment and 15 N-tracing models, denitrification was believed to be the largest source of N 2 O flux relative to nitrification and other N transformations in the subtropical forest soils [58,59]. On the other hand, accelerated nitrification by NH 4 þ additions gradually decreased soil pH, which would result in higher N 2 O/N 2 ratio during soil denitrification [60]. However, other microorganisms (e.g., methane oxidizers, methanogens and denitrifiers) were also involved in the production and consumption of soil CH 4 and N 2 O.…”

Section: Linkages Between Soil Ch 4 and N 2 O Fluxes And Ammoniaoxidimentioning

confidence: 99%

Relationships between ammonia-oxidizing communities, soil methane uptake and nitrous oxide fluxes in a subtropical plantation soil with nitrogen enrichment

Wang

Cheng

Fang

et al. 2016

European Journal of Soil Biology

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a b s t r a c tAmmonia-oxidizers play an essential role in nitrogen (N) transformation and nitrous oxide (N 2 O) emission in forest soils. It remains unclear if ammonia-oxidizers affect interaction between methane (CH 4 ) uptake and N 2 O emission. Our specific goal was to test the impacts of changes in ammoniaoxidizing communities elicited by N enrichment on soil CH 4 uptake and N 2 O emission. Based on a field experiment, two-forms (NH 4 Cl and NaNO 3 ) and two levels (40 and 120 kg N ha À1 yr À1 ) of N were applied in the subtropical plantation forest of southern China. Soil CH 4 and N 2 O fluxes, the abundance and structure of ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB) communities were measured using static chamber-gas chromatography, quantitative PCR (qPCR), and terminalrestriction fragment length polymorphism (T-RFLP). Nitrogen addition tended to inhibit soil CH 4 uptake, but significantly promoted soil N 2 O emission; moreover, these impacts were more significant with NH 4 þ À N than with NO 3 À À N addition. NH 4 Cl addition significantly changed ammonia-oxidizer abundance with an increase in AOA and a decrease in AOB. Nitrogen additions significantly decreased the relative abundance of 329 bp and 421 bp of archaeal amoA gene. Negative relationships occurred between soil CH 4 uptake and AOA abundance and between soil CH 4 uptake and AOA/AOB ratio; however, a positive relationship was found between soil N 2 O emission and AOA abundance. These results indicate that a shift in abundance and composition of ammonia-oxidizing communities is closely linked to changes in soil CH 4 uptake and N 2 O emission under N enrichment. Furthermore, AOA communities play a contrasting role from AOB communities for regulating the fluctuation between soil CH 4 and N 2 O fluxes.

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Denitrification gene pools, transcription and kinetics of NO, N2O and N2 production as affected by soil pH

Cited by 419 publications

References 35 publications

Compaction stimulates denitrification in an urban park soil using 15N tracing technique

Compaction stimulates denitrification in an urban park soil using 15N tracing technique

Effect of pyrene on denitrification activity and abundance and composition of denitrifying community in an agricultural soil

Relationships between ammonia-oxidizing communities, soil methane uptake and nitrous oxide fluxes in a subtropical plantation soil with nitrogen enrichment

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