Dengue virus type 1 clade replacement in recurring homotypic outbreaks

Teoh, Boon‐Teong; Sam, Sing‐Sin; Tan, Kian Lam; Johari, Jefree; Shu, Meng-Hooi; Danlami, Mohammed Bashar; Abd-Jamil, Juraina; MatRahim, NorAziyah; Mahadi, Nor Muhammad; AbuBakar, Sazaly

doi:10.1186/1471-2148-13-213

Cited by 57 publications

(52 citation statements)

References 56 publications

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“…The sequence alignment of individual gene (nsP1, nsP2, nsP3, nsP4, C, E3, E2, 6K and E1) for 49 CHIKV were analyzed using HyPhy package42 implemented in the Datamonkey server43 as previously described44. Prior to analysis, the sequence alignment was checked for duplication of genome sequences, which was removed upon identification.…”

Section: Methodsmentioning

confidence: 99%

Independent Emergence of the Cosmopolitan Asian Chikungunya Virus, Philippines 2012

Tan

Tandoc

et al. 2015

Sci Rep

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Outbreaks involving the Asian genotype Chikungunya virus (CHIKV) caused over one million infections in the Americas recently. The outbreak was preceded by a major nationwide outbreak in the Philippines. We examined the phylogenetic and phylogeographic relationships of representative CHIKV isolates obtained from the 2012 Philippines outbreak with other CHIKV isolates collected globally. Asian CHIKV isolated from the Philippines, China, Micronesia and Caribbean regions were found closely related, herein denoted as Cosmopolitan Asian CHIKV (CACV). Three adaptive amino acid substitutions in nsP3 (D483N), E1 (P397L) and E3 (Q19R) were identified among CACV. Acquisition of the nsP3-483N mutation in Compostela Valley followed by E1-397L/E3-19R in Laguna preceded the nationwide spread in the Philippines. The China isolates possessed two of the amino acid substitutions, nsP3-D483N and E1-P397L whereas the Micronesian and Caribbean CHIKV inherited all the three amino acid substitutions. The unique amino acid substitutions observed among the isolates suggest multiple independent virus dissemination events. The possible biological importance of the specific genetic signatures associated with the rapid global of the virus is not known and warrant future in-depth study and epidemiological follow-up. Molecular evidence, however, supports the Philippines outbreak as the possible origin of the CACV.

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Section: Methodsmentioning

confidence: 99%

Independent Emergence of the Cosmopolitan Asian Chikungunya Virus, Philippines 2012

Tan

Tandoc

et al. 2015

Sci Rep

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“…Therefore, it is important to consider other factors, such as the immunological human response of short-term cross-protection, that could explain the fluctuating pattern of dengue virus (DENV) transmission. [7][8][9][10] The human immune response to DENV infection depends on whether it is a primary or secondary infection. For a primary infection, the host's immune system generates neutralizing antibodies against the infecting serotype that offer lifelong protection.…”

Section: Introductionmentioning

confidence: 99%

Seroprevalence of Neutralizing Antibodies Against Dengue Virus in Two Localities in the State of Morelos, Mexico

Amaya-Larios¹,

Martínez-Vega²,

Mayer³

et al. 2014

The American Society of Tropical Medicine and Hygiene

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Abstract. Humoral immune response against dengue virus (DENV) is an important component in dengue-endemic transmission. We conducted a cross-sectional nested cohort study to determine the seroprevalence and frequency of neutralizing antibodies against DENV serotypes in two endemic localities in the state of Morelos, Mexico. The cohort participants (N = 1,196) were screened to determine previous exposure to DENV. Overall seroprevalence was 76.6% (95% confidence interval [95% CI] = 73.6-79.2), and prevalence of neutralizing antibodies in the 5-to 9-year-old group was 82.5% (95% CI = 67.2-92.7), 45% (95% CI = 29.3-61.5), and 65% (95% CI = 48.3-79.4) for DENV-1, DENV-2, and DENV-3, respectively. For participants older than 10 years, the observed seroprevalence was above 60% for each serotype, except DENV-4 in the 10-to 25-year-old group (42.9%); 81% of humoral responses were multitypic. The outcomes of our study contribute to understanding the immune component of dengue transmission and provide focal information for the evaluation of vaccine candidates under development.

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“…To determine the neutralization capability of SLE patient's sera against DENV, FRNT assays were performed on 82 of the dengue IgM and IgG negative sera of individuals with SLE. Sera were diluted at various dilutions (1/20, 1/80, 1/320, 1/1280) and evaluated for neutralization against DENV‐1, DENV‐2 and DENV‐3.…”

Section: Resultsmentioning

confidence: 99%

Sera of patients with systemic lupus erythematosus cross‐neutralizes dengue viruses

Zainal

Tan

Johari

et al. 2018

Microbiology and Immunology

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Dengue is the most prevalent mosquito‐borne disease in Southeast Asia, where the incidence of systemic lupus erythematosus (SLE) is approximately 30 to 53 per 100,000. Severe dengue, however, is rarely reported among individuals with SLE. Here, whether sera of patients with SLE cross‐neutralize dengue virus (DENV) was investigated. Serum samples were obtained from individuals with SLE who were dengue IgG and IgM serology negative. Neutralization assays were performed against the three major DENV serotypes. Of the dengue serology negative sera of individuals with SLE, 60%, 61% and 52% of the sera at 1/320 dilution showed more than 50% inhibition against dengue type‐1 virus (DENV‐1), DENV‐2 and DENV‐3, respectively. The neutralizing capacity of the sera was significantly greater against DENV‐1 (P < 0.001) and DENV‐3 (P < 0.01) than against DENV‐2 (P < 0.05). Neutralization against the DENV correlated with dengue‐specific IgG serum titers below the cut‐off point for dengue positivity. Depletion of total IgG from the sera of patients with SLE resulted in significant decreases of up to 80% in DENV inhibition, suggesting that IgG plays an important role. However, some of the SLE sera was still able to neutralize DENV, even with IgG titers <0.1 OD absorbance. Our findings suggest that sera of patients with SLE contain IgG, and possibly other type of antibodies, that can cross‐neutralize DENV, which may explain the rarity of severe dengue in individuals with SLE. Further studies, are needed to further substantiate this finding and to elucidate the specific neutralizing epitopes recognized by the sera of individuals with SLE.

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Dengue virus type 1 clade replacement in recurring homotypic outbreaks

Cited by 57 publications

References 56 publications

Independent Emergence of the Cosmopolitan Asian Chikungunya Virus, Philippines 2012

Independent Emergence of the Cosmopolitan Asian Chikungunya Virus, Philippines 2012

Seroprevalence of Neutralizing Antibodies Against Dengue Virus in Two Localities in the State of Morelos, Mexico

Sera of patients with systemic lupus erythematosus cross‐neutralizes dengue viruses

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