Dengue Virus Infection Induces Expansion of a CD14+CD16+ Monocyte Population that Stimulates Plasmablast Differentiation

Kwissa, Marcin; Nakaya, Hidehiko; Onlamoon, Nattawat; Wrammert, Jens; Villinger, François; Perng, Guey Chuen; Yoksan, Sutee; Pattanapanyasat, Kovit; Chokephaibulkit, Kulkanya; Ahmed, Rafi; Pulendran, Bali

doi:10.1016/j.chom.2014.06.001

Cited by 216 publications

(292 citation statements)

References 59 publications

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“…A TLR7/8 ligand (R848) induced the mobilization, activation, and expansion of CD14 ϩ CD16 ϩϩ intermediate monocytes both in peripheral blood and in draining LNs. More recently, we have also demonstrated that CD14 ϩ CD16 ϩϩ intermediate monocytes infected with dengue virus (DENV) help with the differentiation of resting B cells into plasmablasts and enhanced immunoglobulin (IgG and IgM) production via the B cell-activating factor (BAFF), A proliferation-inducing ligand (APRIL), and IL-10 cytokines (56). Consistent with these observations, in our current study, the NP adjuvant led to the significant expansion of CD14 ϩ CD16 ϩϩ intermediate monocytes in peripheral blood at days 1 and 2 postvaccination in comparison with the findings for the alum adjuvant, where no such expansion was seen (Fig.…”

Section: Discussionmentioning

confidence: 99%

Adjuvanting a Simian Immunodeficiency Virus Vaccine with Toll-Like Receptor Ligands Encapsulated in Nanoparticles Induces Persistent Antibody Responses and Enhanced Protection in TRIM5α Restrictive Macaques

Kasturi

Kozlowski

Nakaya

et al. 2017

J Virol

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Our previous work has shown that antigens adjuvanted with ligands specific for Toll-like receptor 4 (TLR4) and TLR7/8 encapsulated in poly(lactic-co-glycolic) acid (PLGA)-based nanoparticles (NPs) induce robust and durable immune responses in mice and macaques. We investigated the efficacy of these NP adjuvants in inducing protective immunity against simian immunodeficiency virus (SIV). Rhesus macaques (RMs) were immunized with NPs containing TLR4 and TLR7/8 agonists mixed with soluble recombinant SIVmac239-derived envelope (Env) gp140 and Gag p55 (protein) or with virus-like particles (VLPs) containing SIVmac239 Env and Gag. NPadjuvanted vaccines induced robust innate responses, antigen-specific antibody responses of a greater magnitude and persistence, and enhanced plasmablast responses compared to those achieved with alum-adjuvanted vaccines. NP-adjuvanted vaccines induced antigen-specific, long-lived plasma cells (LLPCs), which persisted in the bone marrow for several months after vaccination. NP-adjuvanted vaccines induced immune responses that were associated with enhanced protection against repeated low-dose, intravaginal challenges with heterologous SIVsmE660 in animals that carried TRIM5␣ restrictive alleles. The protection induced by immunization with protein-NP correlated with the prechallenge titers of Env-specific IgG antibodies in serum and vaginal secretions. However, no such correlate was apparent for immunization with VLP-NP or alum as the adjuvant. Transcriptional profiling of peripheral blood mononuclear cells isolated within the first few hours to days after primary vaccination revealed that NP-adjuvanted vaccines induced a molecular signature similar to that induced by the live attenuated yellow fever viral vaccine. This systems approach identified early blood transcriptional signatures that correlate with Envspecific antibody responses in vaginal secretions and protection against infection. These results demonstrate the adjuvanticity of the NP adjuvant in inducing persis-

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Section: Discussionmentioning

confidence: 99%

Adjuvanting a Simian Immunodeficiency Virus Vaccine with Toll-Like Receptor Ligands Encapsulated in Nanoparticles Induces Persistent Antibody Responses and Enhanced Protection in TRIM5α Restrictive Macaques

Kasturi

Kozlowski

Nakaya

et al. 2017

J Virol

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“…significant increase of the non-classical in sepsis and viral infections or profound decrease during corticosteroid therapy) [22][23][24][25] and their activation state can influence vascular tone. MO are bone marrow-derived cells that circulate in the blood for 1-3 days, and during this time undergo a process of maturation (from classical through intermediate towards non-classical), which is regulated mainly by MO colonystimulating factor (M-CSF/CSF-1), released, among others, by endothelial cells [26].…”

Section: Discussionmentioning

confidence: 99%

Human monocyte subsets exhibit divergent angiotensin I-converting activity

Rutkowska-Zapała¹,

Suski²,

Szatanek³

et al. 2015

Clinical and Experimental Immunology

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SummaryImmune cells may take part in the renin-angiotensin-aldosterone system (RAAS), which plays a pivotal role in the regulation of vascular tone and blood pressure. The aim of the study was to analyse the expression and activity of angiotensin-converting enzyme type 1 (ACE1) and ACE2 in human monocytes (MO) and their subsets. The highest relative level of ACE1-, as well as ACE2-mRNA expression, was observed in CD14 11 CD16 2 (classical) MO. Moreover, in these cells, mean level of ACE2-mRNA was almost two times higher than that of ACE1-mRNA (11.48 versus 7.073 relative units, respectively). In peripheral blood mononuclear cells (PBMC), MO and classical MO, ACE1 and ACE2 protein expression was stronger compared to other MO subpopulations. The highest level of Ang II generated from Ang I in vitro was observed in classical MO. In this setting, generation of Ang-(1-9) by PBMC and classical MO was higher when compared to the whole MO population (P < 0Á05). The generation rate of vasoprotective Ang-(1-7) was comparable in all analysed cell populations. However, in CD14 1 CD16 11(non-classical) MO, formation of Ang-(1-7) was significantly greater than Ang II (P < 0Á001). We suggest that in physiological conditions MO (but also lymphocytes forming the rest of PBMC pool) may be involved in the regulation of vessel wall homeostasis via the RAAS-related mechanisms. Moreover, non-classical MO, which are associated preferentially with the vascular endothelium, express the vasoprotective phenotype.

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“…ϩ monocytes tend to increase (56). In HIV infection, both nonclassical and intermediate monocytes are infected and expanded.…”

Section: Cd16mentioning

confidence: 99%

Human T Cell Leukemia Virus Type 1 Infection of the Three Monocyte Subsets Contributes to Viral Burden in Humans

Castro-Amarante

Pise-Masison

McKinnon

et al. 2016

J Virol

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A pproximately 2 to 3% of human T cell leukemia virus type 1 (HTLV-1)-infected individuals develop adult T-cell leukemia/lymphoma (ATL) and another 2 to 3% develop HTLV-1-associated myelopathy (HAM)/tropical spastic paraparesis (TSP) in their lifetimes (1-4). In addition to HAM/TSP (5, 6), HTLV-1 is also associated with other inflammatory conditions, such as uveitis (6) Sjögren's syndrome (7), bronchoalveolitis and arthritis (8), and polymyositis (9). It is noteworthy that some patients present with more than one of these inflammatory conditions (10). HTLV-1 primarily infects CD4 ϩ and CD8 ϩ effector and memory T cells and regulatory CD4 ϩ CD25 ϩ T cells (11,12). A high viral DNA burden in peripheral blood mononuclear cells (PBMCs) is a risk factor for HAM/TSP (13) and ATL development (14-16), and patients with HAM/TSP have a higher virus level in the cerebrospinal fluid (CSF) than in the peripheral blood (12). The virus level alone is not sufficient to differentiate symptomatic patients from healthy carriers, suggesting the importance of other factors, including the host immune response (16)(17)(18)(19)(20). HAM/TSP patients present diverse immunological alterations, such as increased levels of spontaneous lymphocyte proliferation (21, 22), tax-specific cytotoxic CD8 ϩ T cell expansion, and the production of high levels of inflammatory cytokines (23)(24)(25). Several studies have also suggested that monocytes are involved in immune reg-

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Dengue Virus Infection Induces Expansion of a CD14+CD16+ Monocyte Population that Stimulates Plasmablast Differentiation

Cited by 216 publications

References 59 publications

Adjuvanting a Simian Immunodeficiency Virus Vaccine with Toll-Like Receptor Ligands Encapsulated in Nanoparticles Induces Persistent Antibody Responses and Enhanced Protection in TRIM5α Restrictive Macaques

Adjuvanting a Simian Immunodeficiency Virus Vaccine with Toll-Like Receptor Ligands Encapsulated in Nanoparticles Induces Persistent Antibody Responses and Enhanced Protection in TRIM5α Restrictive Macaques

Human monocyte subsets exhibit divergent angiotensin I-converting activity

Human T Cell Leukemia Virus Type 1 Infection of the Three Monocyte Subsets Contributes to Viral Burden in Humans

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